The complete mitochondrial genome of Blue Pansy,Junonia orithya(Lepidoptera: Nymphalidae: Nymphalinae)

Abstract

The complete mitochondrial genome (mitogenome) of Junonia orithya Linnaeus (Lepidoptera: Nymphalidae: Nymphalinae) is determined to be 15,214 bp in length, including 37 typical mitochondrial genes and an AT-rich region. Its gene order and orientation are identical to those of other butterfly species. All PCGs are initiated by typical ATN codons, except for cox1 gene which is started by CGA codon. Nine genes use complete termination codon (TAA), whereas the cox1. cox2. nad1 and nad4 genes end with single T. Except for trnS1(AGN), all tRNA genes display typical secondary cloverleaf structures as those of other insects. The 331 bp long AT-rich region contains several features common to the other lepidopterans, such as the ATAGA motif followed by a 18 bp poly-T stretch, two microsatellite-like (TA)9 elements, a 5 bp poly-A stretch immediately upstream of trnM gene.

Taxonomy as a hypothesis: testing the status of the Bermuda buckeye butterfly Junonia coenia bergi (Lepidoptera: Nymphalidae)

“…A phylogeny of the New World Junonia was reconstructed using the full mitochondrial genome sequence from J. coenia bergi BERCLL2 (Peters & Marcus, 2016) and from 21 other Junonia specimens representing 12 species within the genus Junonia that had been sequenced previously (Wu et al , ; McCullagh, 2016; Shi et al , ; Teng et al , ; Vanlalruati et al , , ). The full mitochondrial genomes of four outgroup species ( Yoma sabina , Hypolimnas bolina , Melitaea cinxia , and Kallima inachus ) belonging to the subfamily Nymphalinae (Xu et al , , ; Wu et al , ; Shi et al , ) obtained from GenBank were also included.…”

“…Secondary tRNA structures were analyzed using the default settings of rnastructure software (Reuter & Mathews, 2010) and sequence identity comparisons were carried out using emboss needle (Rice et al, 2000). A phylogeny of the New World Junonia was reconstructed using the full mitochondrial genome sequence from J. coenia bergi BERCLL2 (Peters & Marcus, 2016) and from 21 other Junonia specimens representing 12 species within the genus Junonia that had been sequenced previously (Wu et al, 2014;McCullagh, 2016;Shi et al, 2015a;Teng et al, 2015;Vanlalruati et al, 2015Vanlalruati et al, , 2016. The full mitochondrial genomes of four outgroup species (Yoma sabina, Hypolimnas bolina, Melitaea cinxia, and Kallima inachus) belonging to the subfamily Nymphalinae (Xu et al, 2010a(Xu et al, , 2010bWu et al, 2014;Shi et al, 2015b) obtained from GenBank were also included.…”

A tale of two haplotype groups: evaluating the New WorldJunoniaring species hypothesis using the distribution of divergentCOIhaplotypes

“…In addition to the sequences generated by this study, additional sequences from our earlier work (Borchers & Marcus, ; Gemmell et al ., ) and previously published Old World Junonia COI sequences (Kodandaramaiah & Wahlberg, ; Kodandaramaiah, ; Hebert et al ., ; Shi et al ., ) were included in a phylogenetic analysis. COI sequences from each New World Junonia specimen were compared with each other and duplicate sequences were removed from the analysis (identical sequences are listed in Table S2).…”

“…The aligned COI sequences generated by this study, along with sequences from previously published studies from our laboratory (Borchers & Marcus, ; Gemmell et al ., ) and exemplar COI sequences for Old World outgroup species J. orithya and J. villida (Kodandaramaiah & Wahlberg, ; Hebert et al ., ; Shi et al ., ) (each previously identified as possible sister taxa to the New World Junonia based on morphology and wing colour patterns; Seitz, ; Corbet, ; Vane‐Wright & Tennent, ), were formatted for input into Arlequin 3.5 (Schneider et al ., ). No J. villida sequences in Genbank contain the complete COI barcode, so we used the consensus sequence between a published sequence (NW99‐1) (Kodandaramaiah & Wahlberg, ) and a short J. villida COI sequence (AUS1, collected Sydney, Australia, 15 December1999) that we generated ourselves (DNA Database of Japan (DDBJ) Accession Number AB981649) for network analysis.…”

The complete mitochondrial genome ofEurema hecabe(Lepidoptera: Pieridae: Coliadinae)

“…Its gene order and orientation are identical to those of other butterflies. All PCGs are initiated by a typical ATN codon, except for the CO1 gene, which uses unusual CGA(R) as start codon as observed in most of the other sequenced butterflies (Hao et al, , 2013bKim et al, 2010;Shi et al, 2012Shi et al, , 2013. The nucleotide compositions of the genome are significantly biased toward AT (80.7%), which is well within the range of other sequenced pierids, from 79.7% in Pieris rapae (Mao et al, 2010) , 2012).…”