The complete mitochondrial genome of Bactrocera biguttula (Bezzi) (Diptera: Tephritidae) and phylogenetic relationships with other Dacini

Bactrocera carambolae is one of the approximately 100 sibling species of the Bactrocera dorsalis complex and considered to be very closely related to B. dorsalis. Due to their high morphological similarity and overlapping distribution, as well as to their economic impact and quarantine status, the development of reliable markers for species delimitation between the two taxa is of great importance. Here we present the complete mitochondrial genome of B. carambolae sourced from its native range in Malaysia and its invaded territory in Suriname. The mitogenome of B. carambolae presents the typical organization of an insect mitochondrion. Comparisons of the analyzed B. carambolae sequences to all available complete mitochondrial sequences of B. dorsalis revealed several species-specific polymorphic sites. Phylogenetic analysis based on Bactrocera mitogenomes supports that B. carambolae is a differentiated taxon though closely related to B. dorsalis. The present complete mitochondrial sequences of B. carambolae could be used, in the frame of Integrative Taxonomy, for species discrimination and resolution of the phylogenetic relationships within this taxonomically challenging complex, which would facilitate the application of species-specific population suppression strategies, such as the sterile insect technique.

Section: Sequence Comparisons and Phylogenetic Analysissupporting

confidence: 85%

The Complete Mitochondrial Genome of Bactrocera carambolae (Diptera: Tephritidae): Genome Description and Phylogenetic Implications

Drosopoulou

Syllas

Goutakoli

et al. 2019

“…All PCR and sequencing primers were designed by the authors specifically for this study except for segment S03*, which was amplified and sequenced using arthropod universal DNA barcoding primers [44]. PCR was carried out in 25 μL total volume containing 75 mM Tris-HCl (pH 8.8), 20 mM (NH4)2SO4, 0.01% (v/v) Tween 20 (Fermentas), 3.0 mM MgCl2 (Fermentas), 0.5 mM of each dNTP (Fermentas), 25 [45], as well as completion and curation of published mitochondrial sequences [23]. However, the correct assembly of insect mitogenomes from NGS data can be hampered by lack of adequate reference sequences, particularly in groups where gene rearrangements are common (e.g., Hymenoptera), and complete mitogenomes for some subfamilies are not available (e.g., Opiinae).…”

Section: Sanger Sequencingmentioning

confidence: 99%

“…PCR was carried out in 25 PCR products were purified by treatment with ExonucleaseI (Fermentas) and Shrimp Alkaline Phosphatase (Fermentas), and Sanger-sequenced by Macrogen Europe (Amsterdam, The Netherlands). Mitochondrial DNA is usually overrepresented in Next Generation Sequencing (NGS) reads, allowing for easy retrieval of mitogenomes [45], as well as completion and curation of published mitochondrial sequences [23]. However, the correct assembly of insect mitogenomes from NGS data can be hampered by lack of adequate reference sequences, particularly in groups where gene rearrangements are common (e.g., Hymenoptera), and complete mitogenomes for some subfamilies are not available (e.g., Opiinae).…”

Section: Sanger Sequencingmentioning

confidence: 99%

“…However, the fact that it also accepts Ceratitis capitata (Wiedemann, 1824) as a host under laboratory conditions raises the possibility that it can parasitize other Bactrocera spp. in the wild, particularly Bactrocera biguttula (Bezzi, 1922), a close relative of B. oleae known to utilize African wild olives in South Africa, Namibia, and Kenya [21,23,24], and B. munroi White, 2004 also found it in Kenya on African wild olives [25]. Psyttalia humilis was described by Silvestri (1913) based on specimens reared from pears infested by C. capitata in Constantia, Cape Town (South Africa).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New Mitochondrial Gene Rearrangement in Psyttalia concolor, P. humilis and P. lounsburyi (Hymenoptera: Braconidae), Three Parasitoid Species of Economic Interest

Powell

Caleca

Rhode

et al. 2020

Self Cite

The family Braconidae consists mostly of specialized parasitoids, some of which hold potential in biocontrol of agricultural pests. Psyttalia concolor, Psyttalia humilis and Psyttalia lounsburyi are parasitoids associated with Bactrocera oleae, a major pest of cultivated olives. The native range of Psyttalia concolor is the Mediterranean, and P. humilis and P. lounsburyi are native to sub-Saharan Africa. This study reports the mitochondrial genomes of the three species, thus laying the foundation for mitogenomic analyses in the genus Psyttalia. Comparative mitogenomics within Braconidae showed a novel gene arrangement in Psyttalia in involving translocation and inversion of transfer RNA genes. The placement of Psyttalia in the subfamily Opiinae was well-supported, and the divergence between Psyttalia and its closest relative (Diachasmimorpha longicaudata) was at ~55 MYA [95% highest posterior density (HPD): 34–83 MYA]. Psyttalia lounsburyi occupied the most basal position among the three Psyttalia, having diverged from the other two species ~11 MYA (95% HPD: 6–17 MYA). Psyttalia concolor and P. humilis were recovered as sister species diverged at ~2 MYA (95% HPD: 1.1–3.6 MYA). This phylogeny combining new sequences and a set of 31 other cyclostomes and non-cyclostomes highlights the importance of a comprehensive taxonomic coverage of Braconidae mitogenomes to overcome the lack of robustness in the placement of several subfamilies.

“…Sub-Saharan Africa has a rich assemblage of native insects associated with Oleaceae, including several species of olive fruit flies and olive flea beetles, and a diversity of parasitoid, hyperparasitoid and olive seed wasps [ 3 , 4 , 5 , 6 , 7 ]. Lace bugs feeding on Oleaceae are only found in sub-Saharan Africa: Catoplatus dilatatus Jakovlev (on Olea sp.…”

Section: Introductionmentioning

confidence: 99%

Species Diversity and Phylogenetic Relationships of Olive Lace Bugs (Hemiptera: Tingidae) Found in South Africa

Hlaka

Guilbert

Smit

et al. 2021

Self Cite

Olive lace bugs (Hemiptera: Tingidae) are small sap-sucking insects that feed on wild and cultivated Olea europaea. The diversity of olive lace bug species in South Africa, the most important olive producer on the continent, has been incompletely surveyed. Adult specimens were collected in the Western Cape province for morphological and DNA-based species identification, and sequencing of complete mitogenomes. Cysteochila lineata, Plerochila australis, Neoplerochila paliatseasi and Neoplerochila sp. were found at 12 sites. Intra- and interspecific genetic divergences and phylogenetic clustering in 30 species in 18 genera of Tingidae using new and publicly available DNA barcodes showed high levels of congruity between taxonomic and genetic data. The phylogenetic position of the four species found in South Africa was inferred using new and available mitogenomes of Tingidae. Notably, olive lace bugs formed a cluster of closely related species. However, Cysteochila was non-monophyletic as C. lineata was recovered as a sister species to P. australis whereas Cysteochila chiniana, the other representative of the genus, was grouped with Trachypeplus jacobsoni and Tingis cardui in a different cluster. This result suggests that feeding on O. europaea may have a common origin in Tingidae and warrants future research on potential evolutionary adaptations of olive lace bugs to this plant host.