The complete genome sequence of human adenovirus 84, a highly recombinant new Human mastadenovirus D type with a unique fiber gene

Kaján, Gyözö L.; Kajon, Adriana E.; Pinto, Alexis Castillo; Bartha, Dániel; Arnberg, Niklas

doi:10.1016/j.virusres.2017.09.012

Cited by 16 publications

(15 citation statements)

References 36 publications

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“…Exactly in HAdV-D strains it is often observed that homologous recombination has a driving force in adenovirus evolution (Crawford-Miksza and Schnurr 1996;Gonzalez et al 2015;Ismail et al 2018a;Kaján et al 2017;Robinson et al 2013;Walsh et al 2009). Predominantly, intraspecies recombination events are common, but intergenus recombinants have also been reported already: the head domain of the porcine adenovirus 5 (genus Mastadenovirus) fibre seems to be of atadenoviral origin (Nagy et al 2002), though a porcine atadenovirus has not been discovered yet.…”

Section: Adenovirusesmentioning

confidence: 99%

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

et al. 2019

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Viruses have been infecting their host cells since the dawn of life, and this extremely long-term coevolution gave rise to some surprising consequences for the entire tree of life. It is hypothesised that viruses might have contributed to the formation of the first cellular life form, or that even the eukaryotic cell nucleus originates from an infection by a coated virus. The continuous struggle between viruses and their hosts to maintain at least a constant fitness level led to the development of an unceasing arms race, where weapons are often shuttled between the participants. In this literature review we try to give a short insight into some general consequences or traits of virus-host coevolution, and after this we zoom in to the viral clades of adenoviruses, herpesviruses, nucleo-cytoplasmic large DNA viruses, polyomaviruses and, finally, circoviruses.

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Section: Adenovirusesmentioning

confidence: 99%

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

et al. 2019

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“…Human adenoviruses (HAdVs) cause significant numbers of respiratory, ocular, and gastrointestinal diseases and incidences of severe diseases caused by adenoviruses most occur in immunocompromised individuals or toddlers [ 4 , 5 , 6 ]. Recent outbreaks of adenovirus infections are at least partly due to newly evolving adenovirus types from constant HAdVs molecular evolution [ 2 , 7 , 8 ]. To identify these novel clinical isolates is the very first step to study a new pathogen.…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

Zhang

Mese

Schellhorn

et al. 2020

IJMS

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Recently an increasing number of new adenovirus types associated with type-dependent pathogenicity have been identified. However, identification of these clinical isolates represents the very first step to characterize novel pathogens. For deeper analyses, these adenoviruses need to be further characterized in basic virology experiments or they could be applied in translational research. To achieve this goal, it is essential to get genetic access and to enable genetic modification of these novel adenovirus genomes (deletion, insertion, and mutation). Here we demonstrate a high-throughput approach to get genetic access to new adenoviruses via homologous recombination. We first defined the cloning conditions regarding homology arm-length and input adenoviral genome amounts. Then we cloned four naturally occurring adenoviruses (Ad70, Ad73, Ad74, and Ad75) into easy-to-manipulate plasmids and genetically modified them by reporter gene insertion. Three recombinant adenoviruses (Ad70, Ad73, and Ad74) containing a reporter cassette were successfully reconstituted. These novel reporter-labeled adenoviruses were further characterized using the inserted luciferase reporter with respect to receptor usage, presence of anti-adenovirus antibodies, and tropism in vitro. The identified receptor usage, the relatively low prevalence of anti-adenovirus antibodies, and the various cancer cell line transduction pattern are important features of these new pathogens providing essential information for their therapeutic application.

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“…Human adenoviruses (HAdVs) are non-enveloped and double-strand DNA viruses which have a diameter of 70–90 nanometers and a genome of 35–40 kb. Currently, 90 genotypes have been identified and classified into seven species (A to G), in which types 1–51 were initially characterized according to serum neutralization and hemagglutination-inhibition tests, whereas types 52 and the other following types were identified using the whole-genome sequence as the typing standard [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. All original serotypes have now been whole-genome sequenced.…”

Section: Introductionmentioning

confidence: 99%

Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly

Pan

Yan

Zhang

et al. 2018

Viruses

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In 1955, Human adenovirus type 14 (HAdV-B14p) was firstly identified in a military trainee diagnosed as acute respiratory disease (ARD) in the Netherlands. Fifty years later, a genomic variant, HAdV-B14p1, re-emerged in the U.S. and caused large and fatal ARD outbreaks. Subsequently, more and more ARD outbreaks occurred in Canada, the UK, Ireland, and China, in both military and civil settings. To generate a tool for the efficient characterization of this new genomic variant, a full-length infectious genomic clone of HAdV-B14 was successfully constructed using one-step Gibson Assembly method in this study. Firstly, the full genome of HAdV-B14p1 strain GZ01, the first HAdV-B14 isolate in China, was assembled into pBR322 plasmid by Gibson Assembly. The pBRAdV14 plasmid, generated by Gibson Assembly, was analyzed and verified by PCR, restriction enzymes digestion and the sequencing. Secondly, viruses were rescued from pBRAdV14-transfected A549 cells. The integrity of the rescued viruses was identified by restriction enzyme analysis. The complete sequence of the infectious clone was further sequenced. No mutation was found in the infectious clone during the construction when compared with the parental virus and pBR322 sequences. The direct immunofluorescence assay indicated the expression of the hexon protein. Finally, typical virions were observed; the one-step growth curves further showed that the DNA replication and viral reproduction efficiency of pBRAd14 derived viruses was similar with that of wild-type HAdV-B14 strain. The successful construction of the replication-competent infectious clone of pBRAdV14 facilitates the development of vaccine and antiviral drugs against HAdV-B14, as well as provides a novel strategy for rapid construction of infectious viral clones for other large-genome DNA viruses.

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The complete genome sequence of human adenovirus 84, a highly recombinant new Human mastadenovirus D type with a unique fiber gene

Cited by 16 publications

References 36 publications

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly

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