The complete genome sequence and analysis of vB_VorS-PVo5, a Vibrio phage infectious to the pathogenic bacterium Vibrio ordalii ATCC-33509

Abstract

The bacterium Vibrio ordalii is best known as the causative agent of vibriosis outbreaks in fish and thus recognized for generating serious production losses in aquaculture systems. Here we report for the first time on the isolation and the genome sequencing of phage vB_VorS-PVo5, infectious to Vibrio ordalii ATCC 33509. The features as well as the complete genome sequence and annotation of the Vibrio phage are described; vB_VorS-PVo5 consists of a lineal double stranded DNA totaling ~ 80.6 Kb in length. Considering its ability to lyse Vibrio ordalii ATCC 33509, the phage is likely to gain importance in future aquaculture applications by controlling the pathogen and as such replacing antibiotics as the treatment of choice.

Characterization of the Bacteriophage vB_VorS-PVo5 Infection on Vibrio ordalii: A Model for Phage-Bacteria Adsorption in Aquatic Environments

“…The phage PvB has been described in previous studies (Echeverría-Vega et al, 2016) as a strain belonging to the Siphoviridae family (Order Caudovirales), which groups viruses containing double-stranded DNA chains. It produced lysis plaques of ∼ 2 mm of diameter in the agar plate when infected with VO.…”

“…The phage vB_VorS-PVo5 (PvB) is described as a lytic strain belonging to the Siphoviridae family (Echeverría-Vega et al, 2016). It was obtained from soft tissues of Perumytilus purpuratus (Lamarck 1819) recovered from the coast of Antofagasta, Chile using a previously described methodology (Echeverría-Vega et al, 2019).…”

“…VO is an important pathogen that causes great losses for the aquaculture industry (Schiewe et al, 1981). Their specific phage (PvB) is important because of its potential to be used as an antibacterial agent in phage therapy in aquaculture (Echeverría-Vega et al, 2016). It was isolated from mollusk inhabitants of the Antofagasta coast (Chile).…”