The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces

Li, Kun; Liu, Juanjuan; Zeng, Zhibo; Kulyar, Muhammad Fakhar‐e‐Alam; Wang, Yaping; Li, Aoyun; Bhutta, Zeeshan Ahmad; Aqib, Amjad Islam; Shahzad, Muhammad; Li, Jiakui; Qi, Desheng

doi:10.3390/genes11121527

Cited by 10 publications

(2 citation statements)

References 45 publications

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“…In-depth dissection of gut microbial composition and structure is beneficial for understanding the importance and underlying mechanisms of gut microbiota in intestinal function and host health ( 29 , 30 ). Additionally, high-throughput genomic technology can also conduct a comprehensive analysis of probiotic whole genome sequences, which contribute to exploring potential probiotic mechanisms ( 31 , 32 ). Previous studies indicated that the genome of Bacillus subtilis derived from different sources such as tilapia and soil has been well-characterized and revealed the reason for their probiotic properties ( 33 ).…”

Section: Introductionmentioning

confidence: 99%

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Wang

Lin

et al. 2023

Front. Vet. Sci.

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Probiotics have attracted attention due to their multiple health benefits to the host. Yaks inhabiting the Tibetan plateau exhibit excellent disease resistance and tolerance, which may be associated with their inner probiotics. Currently, research on probiotics mainly focuses on their positive effects on the host, but information regarding their genome remains unclear. To reveal the potential functional genes of Bacillus subtilis isolated from yaks, we sequenced its whole genome. Results indicated that the genomic length of Bacillus subtilis was 866,044,638 bp, with 4,429 coding genes. The genome of this bacteria was composed of one chromosome and one plasmid with lengths of 4,214,774 and 54,527 bp, respectively. Moreover, Bacillus subtilis contained 86 tRNAs, 27 rRNAs (9 16S_rRNA, 9 23S_rRNA, and 9 5S_rRNA), and 114 other ncRNA. KEGG annotation indicated that most genes in Bacillus subtilis were associated with biosynthesis of amino acids, carbon metabolism, purine metabolism, pyrimidine metabolism, and ABC transporters. GO annotation demonstrated that most genes in Bacillus subtilis were related to nucleic acid binding transcription factor activity, transporter activity, antioxidant activity, and biological adhesion. EggNOG uncovered that most genes in Bacillus subtilis were related to energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism. CAZy annotation found glycoside hydrolases (33.65%), glycosyl transferases (22.11%), polysaccharide lyases (3.84%), carbohydrate esterases (14.42%), auxiliary activities (3.36%), and carbohydrate-binding modules (22.59%). In conclusion, this study investigated the genome and genetic properties of Bacillus subtilis derived from yaks, which contributed to understanding the potential prebiotic mechanism of probiotics from the genetic perspective.

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Section: Introductionmentioning

confidence: 99%

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Wang

Lin

et al. 2023

Front. Vet. Sci.

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“…With the advancement of genomic studies, it is possible to make a comprehensive analysis of the probiotic microorganism, from its precise identity to exploring potential probiotic mechanisms, which must be evaluated in detail before its application [13,14]. In the study by D'Rose and Bhat [15], the probiotic potential of Bacillus amyloliquefaciens BTSS3 was investigated through the complete sequence of its genome.…”

Section: Introductionmentioning

confidence: 99%

Bacillus altitudinis 1.4 genome analysis - functional annotation of probiotic properties and immunomodulatory activity

Jankoski

Bach

Fonseca

et al. 2023

Preprint

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Probiotics are live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. They are often used to improve intestinal health. In this study, phenotypic and genotypic methods were used to evaluate the probiotic properties of Bacillus altitudinis 1.4. The isolate was sensitive to all tested antimicrobials and showed a positive result in the hemolysis test. The results also indicated that the spores of B. altitudinis 1.4 were more resistant than the vegetative cells, when evaluated in a simulation of cell viability in the gastrointestinal tract, as well as adhesion to the intestinal mucosa. The isolate was able to self-aggregate and coaggregate with pathogens such as Escherichia coli ATCC 25922 and Salmonella Enteritidis ATCC 13076. Genomic analysis revealed the presence of genes with probiotic characteristics, such as fglA-L, motA, motB, luxS, pgaA-D, exoX, epsG. From this study, it was possible to evaluate the gene expression of pro-inflammatory and anti-inflammatory cytokines for different treatments. Viable vegetative cells of B. altitudinis 1.4 increased the transcription of pro-inflammatory factors, such as TNFα, NFkβ, TLR2, in addition to also increasing the transcription of IL-10, indicating a tendency to stimulate a pro-inflammatory profile. In view of the results presented, B. altitudinis 1.4 showed the potential to be applied in the incorporation of this microorganism in animal feed, since the spores could tolerate the handling and pelleting processes of the feed.

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Complete Genome Sequence of Lactobacillus salivarius AR809, a Probiotic Strain with Oropharyngeal Tract Resistance and Adhesion to the Oral Epithelial Cells

et al. 2022

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The Complete Genome of Probiotic Lactobacillus sakei Derived from Plateau Yak Feces

Cited by 10 publications

References 45 publications

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Complete genome analysis of Bacillus subtilis derived from yaks and its probiotic characteristics

Bacillus altitudinis 1.4 genome analysis - functional annotation of probiotic properties and immunomodulatory activity

Complete Genome Sequence of Lactobacillus salivarius AR809, a Probiotic Strain with Oropharyngeal Tract Resistance and Adhesion to the Oral Epithelial Cells

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