The Complete DNA Sequence of Autographa californica Nuclear Polyhedrosis Virus

Ayres, Martin D.; Howard, Stephen C.; Kuzio, John; López‐Ferber, M.; Possee, Robert D.

doi:10.1006/viro.1994.1380

Cited by 881 publications

(642 citation statements)

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“…The genome of AcMNPV consists of a 131 kb double-stranded circular DNA molecule. 42 Although it was thought that the extra packaging capacity of baculovirus might exceed 20 kb, the actual upper packaging limit of baculovirus capsids has not yet been established. Some investigators have suggested that baculoviruses can merely extend their corkscrew-like capsids to accommodate extra DNA sequences.…”

Section: Discussionmentioning

confidence: 99%

A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus

et al. 2001

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Fully deleted adenovirus vectors (FD-AdVs) would appear to be promising tools for gene therapy. Since these vectors are deleted of all adenoviral genes, they require a helper adenovirus for their propagation. The contamination of the vector preparation by the helper limits the utility of currently existing FD-AdVs in gene therapy applications. We have developed an alternative system for the propagation of FD-AdVs, in which the adenoviral genes essential for replication and packaging of the vector are delivered into producer cells by a baculovirus-adenovirus hybrid. A hybrid baculovirus Bac-B4 was constructed to carry a Cre recombinase-excisable copy of the packaging-deficient adenovirus genome. Although the total size of the DNA insert in Bac-B4 was 38 kb, the genetic structure of this recombinant baculovirus was stable. Bac-B4 gave high yields in Sf9 insect cells, with titers of 5 × 10 8 p.f.u./ml before concentration. Transfection

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Section: Discussionmentioning

confidence: 99%

A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus

et al. 2001

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“…Identical residues are shadowed in dark grey whereas similar residues are in light grey. CNEU is the neuralized homolog of Caenorhabditis elegans (Wilson and al., 1994); DNEU is neuralized from Drosophila melanogaster (Boulianne et al, 1991); IAP homologs are from the following species: IAPC, Cydia pomonella granulosis virus (Crook et al, 1993); IAPO, Orgyia pseudotsugata nuclear polyhedrosis virus (Birnbaum et al, 1994); IAPA, Autographa californica nuclear polyhedrosis virus (Ayres et al, 1994); DIAP, Drosophila melanogaster (Liston et al, 1996); XIAP, HIAP1 and HIAP2, Homo sapiens (Liston et al, 1996) and MIAP2, Mus musculus (Rothe et al, 1995) Fingers: what for?…”

Section: The Central Acidic Domain: a Link With Translation?mentioning

confidence: 99%

Mdm2: keeping p53 under control

1997

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The Mdm2 gene is overexpressed in several human tumors. The oncogenic potential of Mdm2 is partially explained by the inhibition of the activity of the tumor suppressor protein p53. Determination of the threedimensional structure of complexes between Mdm2 and the N-terminal p53 peptide provided a molecular basis for the inhibition of the transcriptional function of p53 by Mdm2. More dramatically, p53 is targeted by Mdm2 for rapid degradation. The Mdm2 gene itself is activated by p53, which gives the opportunity for feed-back control of p53 activity. Keeping p53 under control is most likely the major task of Mdm2 during early development. Recently, evidence was provided for an alternative, p53-independent function of Mdm2.

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“…The apparent size of the MaviNPV genome (111.95 kbp in this study) is smaller than that of AcMNPV (133.9 kbp) (Ayres et al, 1994) and BmNPV (128.4 kbp) (Gomi et al, 1999). Based on the sequences of 29 baculoviral core genes in phylogenetic analyses in this study, MaviNPV belongs to group I NPVs and is closely related to AcMNPV and BmNPV.…”

Section: Introductionmentioning

confidence: 62%

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

Chen

Lee

et al. 2008

Journal of General Virology

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The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C content, and contained 126 open reading frames (ORFs) encoding predicted proteins of over 50 aa. The gene content and gene order of MaviNPV have the highest similarity to those of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome with fewer auxiliary genes than the AcMNPV type species. Except for one ORF (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is the first lepidopteranspecific baculovirus to lack homologues of vfgf and odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the MaviNPV genome, and these contained a total of 44 imperfect palindromes. Phylogenetic analysis of the whole genome revealed that MaviNPV was separated from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before these two viral species diverged from each other. Moreover, replication of MaviNPV in several cell lines and an egfp-MaviNPV infection assay revealed that IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.

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The Complete DNA Sequence of Autographa californica Nuclear Polyhedrosis Virus

Cited by 881 publications

References 0 publications

A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus

A novel system for the production of fully deleted adenovirus vectors that does not require helper adenovirus

Mdm2: keeping p53 under control

Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus

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