The complete DNA sequence of the mitochondrial genome of the dermatophyte fungus Epidermophyton floccosum

Abstract

We report here the complete nucleotide sequence of the 30.9-kb mitochondrial genome of the dermatophyte fungus Epidermophyton floccosum. All genes are encoded on the same DNA strand and include seven subunits of the reduced nicotinamide adenine dinucleotide ubiquinone oxireductase (nad1, nad2, nad3, nad4, nad4L, nad5, and nad6), three subunits of cytochrome oxidase (cox1, cox2, and cox3), apocytochrome b (cob), three subunits of ATP synthase (atp6, atp8, and atp9), the small and large ribosomal RNAs (rns and rnl), and 25 tRNAs. A ribosomal protein gene (rps5) is present as an intronic ORF in the large ribosomal subunit. The genes coding for cob and cox1 carry one intron and nad5 carries two introns with ORFs. The mtDNA of E. floccosum has the same gene order as Trichophyton rubrum mtDNA, with the exception of some tRNA genes. Maximum likelihood phylogenetic analysis confirms T. rubrum as a close relative of E. floccosum. This is the first complete mitochondrial sequence of a species of the order Onygenales. This sequence is available under GenBank accession number AY916130.

Intraspecific comparison and annotation of two complete mitochondrial genome sequences from the plant pathogenic fungus Mycosphaerella graminicola

“…1). As in other filamentous fungi, several tRNA genes flanked the rnl gene (Table 3, Tambor et al, 2006). In M. graminicola, these tRNA genes had an order similar to that of Eurotiomycetes and generally followed a conserved pattern found in other fungi (Table 3, Ghikas et al, 2006).…”

Dothideomycete–Plant Interactions Illuminated by Genome Sequencing and EST Analysis of the Wheat Pathogen Stagonospora nodorum

“…The 27 tRNA genes clustered into five groups, with the two larger tRNA gene clusters flanking rnl, a pattern common to other fungal mitochondrial DNAs (mtDNAs) (Tambor et al, 2006). The tRNA gene cluster 59 to rnl had GDS 1 WIS 2 P as a consensus, with its closest sequenced relative M. graminicola (GenBank accession number EU090238), while the 39-downstream consensus was EAFLQHM, having many tRNA genes in the same order found in other Ascomycetes.…”

The human fungal pathogenParacoccidioides brasiliensis(Onygenales: Ajellomycetaceae) is a complex of two species: phylogenetic evidence from five mitochondrial markers

“…Although other introns are present in the Paracoccidioides mitochondrial genome (Cardoso et al., 2007), this particular intron of the cob gene has not been reported previously for the P. brasiliensis species complex, but is known to occur in other fungi, which can contain one or several introns in their cob genes (Lang et al., 1985; Seraphin et al., 1987; Biswas et al., 2001). Others have shown that the presence of organellar introns (introns from groups 1 and 2) in fungal mitochondrial genomes can account for intra‐ and interspecies size variation in fungal mtDNA (Gray, 1989; Kouvelis et al., 2004; Tambor et al., 2006).…”