The Complete DNA Sequence of the Ectocarpus siliculosus Virus EsV-1 Genome

Delaroque, Nicolas; Müller, Dieter G.; Bothe, Gordana; Pohl, Thomas; Knippers, Rolf; Boland, Wilhelm

doi:10.1006/viro.2001.1028

Cited by 115 publications

(102 citation statements)

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“…All characteristic structural and sequence features of EsGMD1 are highly conserved between different order of brown algae like the Ectocarpels, Fucales, and Laminariales. Ectocarpus can be infected by a large genome virus EsV-1 (335-kbp genome) which has distant copy of a UGD or GMD gene in its genome (19). Such a virus could have caused a lateral gene transfer in the past, in which genes for an alginate pathway were transferred into a brown alga.…”

Section: Discussionmentioning

confidence: 99%

Characterization of GDP-mannose Dehydrogenase from the Brown Alga Ectocarpus siliculosus Providing the Precursor for the Alginate Polymer

Tenhaken

Voglas

Cock³

et al. 2011

Journal of Biological Chemistry

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Alginate is a major cell wall polymer of brown algae. The precursor for the polymer is GDP-mannuronic acid, which is believed to be derived from a four-electron oxidation of GDPmannose through the enzyme GDP-mannose dehydrogenase (GMD). So far no eukaryotic GMD has been biochemically characterized. We have identified a candidate gene in the Ectocarpus siliculosus genome and expressed it as a recombinant protein in Escherichia coli. The GMD from Ectocarpus differs strongly from related enzymes in bacteria and is as distant to the bacterial proteins as it is to the group of UDP-glucose dehydrogenases. It lacks the C-terminal ϳ120 amino acid domain present in bacterial GMDs, which is believed to be involved in catalysis. The GMD from brown algae is highly active at alkaline pH and contains a catalytic Cys residue, sensitive to heavy metals. The product GDP-mannuronic acid was analyzed by HPLC and mass spectroscopy. The K m for GDP-mannose was 95 M, and 86 M for NAD ؉ . No substrate other than GDP-mannose was oxidized by the enzyme. In gel filtration experiments the enzyme behaved as a dimer. The Ectocarpus GMD is stimulated by salts even at low molar concentrations as a possible adaptation to marine life. It is rapidly inactivated at temperatures above 30°C.

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Section: Discussionmentioning

confidence: 99%

Characterization of GDP-mannose Dehydrogenase from the Brown Alga Ectocarpus siliculosus Providing the Precursor for the Alginate Polymer

Tenhaken

Voglas

Cock³

et al. 2011

Journal of Biological Chemistry

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“…Viruses from several other genera in the phycodnavirus family have been sequenced, and members from two of them encode K + channel proteins, including viruses that infect the filamentous brown alga Ectocarpus siliculosus (Delaroque et al, 2001) and viruses that infect some of the smallest known algae (the Prasinophytes), Ostreococcus (Derelle et al, 2008;Weynberg et al, 2009), Micromonas (Moreau et al, 2010), and Bathycoccus spp. (Moreau et al, 2010).…”

Section: Channel and Transporter Gene Products Coded By Algal Virusesmentioning

confidence: 99%

Potassium Ion Channels: Could They Have Evolved from Viruses?

et al. 2013

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“…Sequencing also revealed the presence of an integrated copy of a large DNA virus, closely related to the Ectocarpus phaeovirus EsV-1 (ref. 8; Fig. 2a).…”

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The Ectocarpus genome and the independent evolution of multicellularity in brown algae

Cock

Sterck

Rouzé

et al. 2010

Nature

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Brown algae (Phaeophyceae) are complex photosynthetic organisms with a very different evolutionary history to green plants, to which they are only distantly related(1). These seaweeds are the dominant species in rocky coastal ecosystems and they exhibit many interesting adaptations to these, often harsh, environments. Brown algae are also one of only a small number of eukaryotic lineages that have evolved complex multicellularity (Fig. 1). We report the 214 million base pair (Mbp) genome sequence of the filamentous seaweed Ectocarpus siliculosus (Dillwyn) Lyngbye, a model organism for brown algae(2-5), closely related to the kelps(6,7) (Fig. 1). Genome features such as the presence of an extended set of light-harvesting and pigment biosynthesis genes and new metabolic processes such as halide metabolism help explain the ability of this organism to cope with the highly variable tidal environment. The evolution of multicellularity in this lineage is correlated with the presence of a rich array of signal transduction genes. Of particular interest is the presence of a family of receptor kinases, as the independent evolution of related molecules has been linked with the emergence of multicellularity in both the animal and green plant lineages. The Ectocarpus genome sequence represents an important step towards developing this organism as a model species, providing the possibility to combine genomic and genetic(2) approaches to explore these and other(4,5) aspects of brown algal biology further

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The Complete DNA Sequence of the Ectocarpus siliculosus Virus EsV-1 Genome

Cited by 115 publications

References 68 publications

Characterization of GDP-mannose Dehydrogenase from the Brown Alga Ectocarpus siliculosus Providing the Precursor for the Alginate Polymer

Characterization of GDP-mannose Dehydrogenase from the Brown Alga Ectocarpus siliculosus Providing the Precursor for the Alginate Polymer

Potassium Ion Channels: Could They Have Evolved from Viruses?

The Ectocarpus genome and the independent evolution of multicellularity in brown algae

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