The complete chloroplast genome sequence of <i>Salix kochiana</i> Trautv. and its phylogenetic analysis

Wang, Jing; Yu, Zicheng; Xu, Yunbi; Wan, Jie; Wang, Zhengxuan; Li, Xiaoping

doi:10.1080/23802359.2022.2087555

Cited by 2 publications

(1 citation statement)

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“…With the development of high-throughput sequencing technology and low sequencing cost, increasing amounts of cp genomic data have been accumulated (Hatmaker et al, 2020;Shidhi et al, 2021;Wei et al, 2021;Zhang et al, 2021). To date, the cp genome of Salix has been reported (Chen et al, 2019;Guo et al, 2021;Li et al, 2021;Gulyaev et al, 2022;Wang et al, 2022). The published cp genomic data of these species offer important references for related research (Lauron-Moreau et al, 2015;Zong et al, 2019;Meucci et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements

et al. 2022

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Salix floderusii is a rare alpine tree species in the Salix genus. Unfortunately, no extensive germplasm identification, molecular phylogeny, and chloroplast genomics of this plant have been conducted. We sequenced the chloroplast (cp) genome of S. floderusii for the first time using second-generation sequencing technology. The cp genome was 155,540 bp long, including a large single-copy region (LSC, 84,401 bp), a small single-copy region (SSC, 16,221 bp), and inverted repeat regions (IR, 54,918 bp). A total of 131 genes were identified, including 86 protein genes, 37 tRNA genes, and 8 rRNA genes. The S. floderusii cp genome contains 1 complement repeat, 24 forward repeats, 17 palindromic repeats, and 7 reverse repeats. Analysis of the IR borders showed that the IRa and IRb regions of S. floderusii and Salix caprea were shorter than those of Salix cinerea, which may affect plastome evolution. Furthermore, four highly variable regions were found, including the rpl22 coding region, psbM/trnD-GUC non-coding region, petA/psbJ non-coding region, and ycf1 coding region. These high variable regions can be used as candidate molecular markers and as a reference for identifying future Salix species. In addition, phylogenetic analysis indicated that the cp genome of S. floderusii is sister to Salix cupularis and belongs to the Subgenus Vetrix. Genes (Sf-trnI, Sf-PpsbA, aadA, Sf-TpsbA, Sf-trnA) obtained via cloning were inserted into the pBluescript II SK (+) to yield the cp expression vectors, which harbored the selectable marker gene aadA. The results of a spectinomycin resistance test indicated that the cp expression vector had been successfully constructed. Moreover, the aadA gene was efficiently expressed under the regulation of predicted regulatory elements. The present study provides a solid foundation for establishing subsequent S. floderusii cp transformation systems and developing strategies for the genetic improvement of S. floderusii.

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confidence: 99%

The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements

et al. 2022

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Phytochemical and Antioxidant Characterization of Extracts from Unexplored Medicinal Plants Salix schwerinii and Salix kochiana

Lee,

Song,

Son

et al. 2023

Horticulturae

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For a long time, species of the genus Salix have been widely utilized and studied as medicinal plants; however, the biological activity and phytochemical composition of Salix schwerinii (SS) and S. kochiana (SK) have not been studied at all. This study investigated the antioxidant properties of SS and SK extracts and detected phytochemical compounds in the extracts. The results showed that the antioxidant activities (IC50) of SS extract, SK extract, and ascorbic acid (reference) were as follows, respectively: 169.8, 79.8, and 71.2 μg mL−1 for ABTS cation radical scavenging and 38.4, 26.2, and 9.3 μg mL−1 for DPPH free radical scavenging. The results imply that SK has a high potential as a natural antioxidant. The phytochemical compositions of extracts (mg g−1) were analyzed as follows: SS extracts, 217.7 phenolics (1.54 catechin, 0.86 syringic acid, 0.46 luteolin, and others) and 5.06 salicin; SK extracts, 71.0 phenolics (0.54 catechin, 0.28 myricetin, 0.12 salicylic acid, and others) and 2.11 salicin. Compared to previous studies, the present findings go further to highlight that SS deserves attention as a novel source of salicin. The present study highlights the need for further studies on the aspects of medicinal functions of the extracts, bioprocess design for efficient phytochemical extraction, and applications of bioactive substances.

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The complete chloroplast genome sequence of Salix kochiana Trautv. and its phylogenetic analysis

Cited by 2 publications

References 12 publications

The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements

The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements

Phytochemical and Antioxidant Characterization of Extracts from Unexplored Medicinal Plants Salix schwerinii and Salix kochiana

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