The complete chloroplast genome of Pinus yunnanensis Franchet (Pinaceae)

Abstract

Pinus yunnanensis is distributed in Southwestern China which is one of the main forest tree species. It is used for construction, railway sleepers, boards, furniture, and wood pulp. Due to the genetic infiltration hybridization between P. yunnanensis with P. densata and P. kesiya, the classification boundary between these groups is blurred and the identification is difficult. In this study, the complete chloroplast (cp) genome sequence of P. yunnanensis was determined using next-generation sequencing. The entire cp genome was determined to be 119,707 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 65,619 and 53,098 bp, respectively, which were separated by a pair of 495bp inverted repeat (IR) regions. The genome contained 116 genes, including 76 protein-coding genes, 36 tRNA genes, and 4 rRNA genes. The overall GC content of the genome is 38.5%. A phylogenetic tree reconstructed by 23 chloroplast genomes reveals that P. yunnanensis is most related with P. kesiya.

New Insight into Taxonomy of European Mountain Pines, Pinus mugo Complex, Based on Complete Chloroplast Genomes Sequencing

“…The IR regions, on the other hand, are strongly reduced and, in the case of the representatives of the Pinus mugo complex, they are only 365 bp, which is one of the shortest so far described in the Pinaceae family. For comparison, the IR length in Pinus taeda (KC427273) is 485 bp, and for Pinus sylvestris (KR476379), Pinus densiflora (MK285358) or Pinus yunnanensis (MK007968) is exactly 495 bp [ 42 , 43 ]. For other species, differences in IR lengths are also observed, and several studies report that contraction and expansion of IR regions are quite common phenomena in plants [ 44 ].…”