The complete cDNA and amino acid sequence of human apolipoprotein B-100.

Chen, S. H.; Yang, Chao; Chen, P. F.; Setzer, David R.; Tanimura, Masako; Li, W. H.; Gotto, Antonio M.; Chan, Lawrence

doi:10.1016/s0021-9258(18)69248-8

Cited by 207 publications

(31 citation statements)

References 24 publications

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“…Moreover, ApoB derived peptides have been already used in vaccine preparations to treat atherosclerosis [23]. Here, we applied our in silico analysis method to a human ApoB isoform [26,27] and identified a novel ''cryptic" HDP (region 887-922). To the best of our knowledge, this ApoB region has never been analysed before, since all the previously identified biologically active ApoB peptides are far from the high scoring region identified in the present work [22,23].…”

Section: Discussionmentioning

confidence: 99%

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Novel human bioactive peptides identified in Apolipoprotein B: Evaluation of their therapeutic potential

Gaglione

Dell’Olmo

Bosso

et al. 2017

Biochemical Pharmacology

124

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Section: Discussionmentioning

confidence: 99%

“…On the basis of the interesting results obtained in the case of human ApoE, we applied our in silico analysis method to a human Apolipoprotein B (ApoB) isoform [26,27]. In Fig.…”

Section: Introductionmentioning

confidence: 99%

Novel human bioactive peptides identified in Apolipoprotein B: Evaluation of their therapeutic potential

Gaglione

Dell’Olmo

Bosso

et al. 2017

Biochemical Pharmacology

124

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“…One such therapeutic approach is to block or reduce binding of ApoB to HSPGs in the subendothelial spaces. ApoB exists in two isoforms on lipoprotein particles, ApoB-48 and ApoB-100, both of which are encoded by the same gene and play a crucial role in lipid homeostasis [221]. ApoB-100 is a very large protein containing 4536 amino acids [222].…”

Section: Remnant Particles and Their Role In Atherosclerosismentioning

confidence: 99%

The Importance of Lipoprotein Lipase Regulation in Atherosclerosis

et al. 2021

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Lipoprotein lipase (LPL) plays a major role in the lipid homeostasis mainly by mediating the intravascular lipolysis of triglyceride rich lipoproteins. Impaired LPL activity leads to the accumulation of chylomicrons and very low-density lipoproteins (VLDL) in plasma, resulting in hypertriglyceridemia. While low-density lipoprotein cholesterol (LDL-C) is recognized as a primary risk factor for atherosclerosis, hypertriglyceridemia has been shown to be an independent risk factor for cardiovascular disease (CVD) and a residual risk factor in atherosclerosis development. In this review, we focus on the lipolysis machinery and discuss the potential role of triglycerides, remnant particles, and lipolysis mediators in the onset and progression of atherosclerotic cardiovascular disease (ASCVD). This review details a number of important factors involved in the maturation and transportation of LPL to the capillaries, where the triglycerides are hydrolyzed, generating remnant lipoproteins. Moreover, LPL and other factors involved in intravascular lipolysis are also reported to impact the clearance of remnant lipoproteins from plasma and promote lipoprotein retention in capillaries. Apolipoproteins (Apo) and angiopoietin-like proteins (ANGPTLs) play a crucial role in regulating LPL activity and recent insights into LPL regulation may elucidate new pharmacological means to address the challenge of hypertriglyceridemia in atherosclerosis development.

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“…ApoB aids in the assembly, secretion, transport, and cellular uptake of lipoproteins. In mammals, ApoB is expressed in the liver and small intestine in two different isoforms, ApoB100 and ApoB48, which represent the full-length and N-terminal 48% portion of the full-length protein, respectively (Chen et al, 1988;Chen et al, 1987;Chen et al, 1986;Hussain et al, 2005;Hussain et al, 1996;Powell et al, 1987). Not surprisingly, there is a direct correlation between circulating ApoB and CAD: Higher levels cause CAD, whereas lower levels are associated with normal homeostasis or, if severely limited, with hypobetalipoproteinemia, which presents as delayed development, hepatomegaly, steatorrhea, and cytolysis (Jang et al, 2020;Welty, 2020).…”

Section: Introductionmentioning

confidence: 99%

Hsp40s play distinct roles during the initial stages of apolipoprotein B biogenesis

Kumari

Fisher

Brodsky

2022

MBoC

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Apolipoprotein B (ApoB) is the primary component of atherogenic lipoproteins, which transport serum fats and cholesterol. Therefore, elevated levels of circulating ApoB are a primary risk factor for cardiovascular disease. During ApoB biosynthesis in the liver and small intestine under nutrient-rich conditions, ApoB cotranslationally translocates into the endoplasmic reticulum (ER) and is lipidated and ultimately secreted. Under lipid-poor conditions, ApoB is targeted for ER Associated Degradation (ERAD). Although prior work identified select chaperones that regulate ApoB biogenesis, the contributions of cytoplasmic Hsp40s are undefined. To this end, we screened ApoB-expressing yeast and determined that a class A ER-associated Hsp40, Ydj1, associates with and facilitates the ERAD of ApoB. Consistent with these results, a homologous Hsp40, DNAJA1, functioned similarly in rat hepatoma cells. DNAJA1 deficient cells also secreted hyperlipidated lipoproteins, in accordance with attenuated ERAD. In contrast to the role of DNAJA1 during ERAD, DNAJB1—a class B Hsp40—helped stabilize ApoB. Depletion of DNAJA1 and DNAJB1 also led to opposing effects on ApoB ubiquitination. These data represent the first example in which different Hsp40s exhibit disparate effects during regulated protein biogenesis in the ER, and highlight distinct roles that chaperones can play on a single ERAD substrate.

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The complete cDNA and amino acid sequence of human apolipoprotein B-100.

Cited by 207 publications

References 24 publications

Novel human bioactive peptides identified in Apolipoprotein B: Evaluation of their therapeutic potential

Novel human bioactive peptides identified in Apolipoprotein B: Evaluation of their therapeutic potential

The Importance of Lipoprotein Lipase Regulation in Atherosclerosis

Hsp40s play distinct roles during the initial stages of apolipoprotein B biogenesis

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