The complete and symmetric transcription of the main non coding region of rat mitochondrial genome: in vivo mapping of heavy and light transcripts

Sbisà, Elisabetta; Nardelli, Maria; Tanzariello, F.; Tullo, Apollonia; Saccone, C.

doi:10.1007/bf00312616

Cited by 51 publications

(25 citation statements)

References 27 publications

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“…'hese data have led us to consider the processing mechanism s a further possible regulatory element in the expression of the nt genome. In addition to the precursor RNAs, we have iden-tified stable antisense RNA species in the two regulatory regions, D-loop and Ori L, and we have suggested their possible regulatory role [7,5].…”

Section: Introductionmentioning

confidence: 90%

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

et al. 1994

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bstract We have characterized the transcriptional pattern of the rat mitochondrial ND6containing region in vivo. We have identified a stable nyadenylated RNA species complementary for the full length of the ND6 mRNA. The analysis of the ND5 region has revealed the presence of I antisense RNA only at its 3' end. The presence of these stable antisense species complementary to structural genes is intriguing and suggests a rssible regulatory function. The quantitative analyses have demonstrated that the H transcripts, both codogenic and non-codogenic, are more stable an the L transcriptswe have defined the 5' end of the ND6 mRNA at the level of the ATG downstream of the tRNAo'". The mapping of the ND1 end has demonstrated that GTG is the first codon of the mRNA. Our findings suggest that the post-transcriptional mechanisms involved in the pression of the mt genome are much more numerous and complex than those already described in the literature.

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Section: Introductionmentioning

confidence: 90%

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

et al. 1994

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“…However, this trend only applied for mtETC components that are encoded by the nuclear genome; the subset of mtETC transcripts encoded by the mitochondrial genome showed transient downregulation. Mitochondrial genome replication and mitochondrial transcription are both initiated by transcriptional machinery primed in the D-loop (Bonawitz et al, 2006), and Dloop transcripts are adenylated (Sbisà et al, 1990;Vijayasarathy et al, 1995;Camasamudram et al, 2003), so a decrease in D-loop transcript abundance could reflect decreased mtDNA synthesis and/or decreased transcription of the mitochondrial genome.…”

Section: Mitochondrion Functionmentioning

confidence: 99%

Salinity- and population-dependent genome regulatory response during osmotic acclimation in the killifish (Fundulus heteroclitus) gill

Whitehead

Roach

Zhang

et al. 2012

Journal of Experimental Biology

128

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SUMMARYThe killifish Fundulus heteroclitus is abundant in osmotically dynamic estuaries and it can quickly adjust to extremes in environmental salinity. We performed a comparative osmotic challenge experiment to track the transcriptomic and physiological responses to two salinities throughout a time course of acclimation, and to explore the genome regulatory mechanisms that enable extreme osmotic acclimation. One southern and one northern coastal population, known to differ in their tolerance to hypo-osmotic exposure, were used as our comparative model. Both populations could maintain osmotic homeostasis when transferred from 32 to 0.4p.p.t., but diverged in their compensatory abilities when challenged down to 0.1p.p.t., in parallel with divergent transformation of gill morphology. Genes involved in cell volume regulation, nucleosome maintenance, ion transport, energetics, mitochondrion function, transcriptional regulation and apoptosis showed population-and salinity-dependent patterns of expression during acclimation. Network analysis confirmed the role of cytokine and kinase signaling pathways in coordinating the genome regulatory response to osmotic challenge, and also posited the importance of signaling coordinated through the transcription factor HNF-4. These genome responses support hypotheses of which regulatory mechanisms are particularly relevant for enabling extreme physiological flexibility. Supplementary material available online at

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“…The presence of such signals has been inferred based on RNase mapping experiments in rats (Sbisa et al 1990); however, no mechanistic details are known for this or for other animal species.…”

Section: Gene Rearrangement Mechanismmentioning

confidence: 99%

Complete mtDNA Sequences of Two Millipedes Suggest a New Model for Mitochondrial Gene Rearrangements: Duplication and Nonrandom Loss

Lavrov

Boore²,

Brown³

2002

Molecular Biology and Evolution

254

193

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We determined the complete mtDNA sequences of the millipedes Narceus annularus and Thyropygus sp. (Arthropoda: Diplopoda) and identified in both genomes all 37 genes typical for metazoan mtDNA. The arrangement of these genes is identical in the two millipedes, but differs from that inferred to be ancestral for arthropods by the location of four genes/gene clusters. This novel gene arrangement is unusual for animal mtDNA, in that genes with opposite transcriptional polarities are clustered in the genome and the two clusters are separated by two non-coding regions. The only exception to this pattern is the gene for cysteine tRNA, which is located in the part of the genome that otherwise contains all genes with the opposite transcriptional polarity. We suggest that a mechanism involving complete mtDNA duplication followed by the loss of genes, predetermined by their transcriptional polarity and location in the genome, could generate this gene arrangement from the one ancestral for arthropods. The proposed mechanism has important implications for phylogenetic inferences that are drawn on the basis of gene arrangement comparisons.

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The complete and symmetric transcription of the main non coding region of rat mitochondrial genome: in vivo mapping of heavy and light transcripts

Cited by 51 publications

References 27 publications

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

Transcription of rat mitochondrial NADH‐dehydrogenase subunits presence of antisense and precursor RNA species

Salinity- and population-dependent genome regulatory response during osmotic acclimation in the killifish (Fundulus heteroclitus) gill

Complete mtDNA Sequences of Two Millipedes Suggest a New Model for Mitochondrial Gene Rearrangements: Duplication and Nonrandom Loss

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