The competitive equilibrium between aluminium and ferric ions for the binding sites of transferrin

Cochran, M.; Coates, John H.; Neoh, S. H.

doi:10.1016/0014-5793(84)80926-6

Cited by 71 publications

(25 citation statements)

References 8 publications

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“…Aluminum was not capable of interfering with the hematopoietic progenitor cell utilization of iron already bound to transferrin. These observations are compatible with previous evidence that demonstrates that transferrin is the major binding protein of aluminum in serum (41)(42)(43). Given the stability constants of aluminum and iron for transferrin (- 1015 mol/liter and 1020 mol/liter, respectively) (43,44), the presence of iron-bound transferrin or competition between iron and aluminum for transferrin would lead to diminished aluminum binding.…”

Section: Discussionsupporting

confidence: 91%

Aluminum inhibits erythropoiesis in vitro.

Mladenovic¹

1988

J. Clin. Invest.

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Anemia has been associated with aluminum intoxication in patients on chronic dialysis and in animals. In studies presented here, in vitro human erythroid culture was used to delineate the effects of aluminum on normal hemnatopoiesis. Aluminum by itself in routine culture, even at very high levels (1,035 ng/ml), did not significantly affect erythroid colony growth. The addition of human transferrin to the culture, however, resulted in a marked dose-dependent inhibition of erythroid, but not myeloid colony growth. At all doses, CFU-E progenitors showed greater inhibition than burst-forming units (BFU-E). Aluminum inhibition was not overcome by increasing the dose of erythkopoietin or adding additional burst-promotihg activity to the culture. Inhibition by aluminum was directly related to the number of binding sites on transferrin in the culture, and was not observed in the presence of fully ironsaturated transferrin.

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Section: Discussionsupporting

confidence: 91%

Aluminum inhibits erythropoiesis in vitro.

Mladenovic¹

1988

J. Clin. Invest.

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“…One study found that a greater percentage of the aluminum added to serum from renally impaired rabbits was free than in serum from normal rabbits (82 (79) reported that the serum transferrin concentration in dialysis patients is about 30 pM.…”

Section: Kinetics Ofaluminum Uptake By Cellsmentioning

confidence: 99%

“…Transferrin, which appears to be the major ligand for aluminum in serum, is a protein of [76][77][78][79][80]. It is similar in size and amino-acid sequence to albumin, but binds metals (particularly iron) with high specificity at two independent binding sites with similar metal-binding affinities.…”

Section: Specific Sources Of Exposurementioning

confidence: 99%

The biological speciation and toxicokinetics of aluminum.

DeVoto

Yokel

1994

Environ Health Perspect

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This review discusses recent literature on the chemical and physiological factors that influence the absorption, distribution, and excretion of aluminum in mammals, with particular regard to gastrointestinal absorption and speciation in plasma. Humans encounter aluminum, a ubiquitous yet highly insoluble element in most forms, in foods, drinking water, and pharmaceuticals. Exposure also occurs by inhalation of dust and aerosols, particularly in occupational settings. Absorption from the gut depends largely on pH and the presence of complexing ligands, particularly carboxylic acids, with which the metal can form absorbable neutral aluminum species. Uremic animals and humans experience higher than normal body burdens of aluminum despite increased urinary clearance of the metal. In plasma, 80-90% of aluminum binds to transferrin, an iron-transport protein for which receptors exist in many tissue. The remaining fraction of plasma aluminum takes the form of small-molecule hydroxy species and small complexes with carboxylic acids, phosphate, and, to a much lesser degree, amino acids. Most of these species have not been observed in vivo but are predicted from equilibrium models derived from potentiometric methods and NMR investigations. These models predict that the major small-molecule aluminum species under plasma conditions are charged and hence unavailable for uptake into tissues.

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“…Tf has recently been shown to specifically bind Al3+ ions with a high affinity, approaching its affinity for iron (Fe3+) (3)(4)(5). Tf also specifically'binds a variety of other metal ions, such as gallium, indium, manganese, and zinc ' (6)(7)(8).…”

mentioning

confidence: 99%

Aluminum access to the brain: a role for transferrin and its receptor.

Roskams

Connor

1990

Proc. Natl. Acad. Sci. U.S.A.

206

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The toxicity of aluminum in plant and animal cell biology is well established, although poorly understood. Several recent studies have identified aluminum as a potential, although highly controversial, contributory factor in the pathology of Alzheimer disease, amyotrophic lateral sclerosis, and dialysis dementia. For example, aluminum has been found in high concentrations in senile plaques and neurofibrillary tangles, which occur in the brains of subjects with Alzheimer disease. However, a mechanism for the entry of aluminum (Al3+) into the cells of the central nervous system (CNS) has yet to be found. Here we describe a possible route of entry for aluminum into the cells of the CNS via the same high-affmnity receptor-ligand system that has been postulated for iron (Fe3 ) delivery to neurons and glial cells. These results suggest that aluminum is able to gain access to the central nervous system under normal physiological conditions. Furthermore, these data suggest that the interaction between transferrin and its receptor may function as a general metal ion regulatory system in the CNS, extending beyond its postulated role in iron regulation.

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The competitive equilibrium between aluminium and ferric ions for the binding sites of transferrin

Cited by 71 publications

References 8 publications

Aluminum inhibits erythropoiesis in vitro.

Aluminum inhibits erythropoiesis in vitro.

The biological speciation and toxicokinetics of aluminum.

Aluminum access to the brain: a role for transferrin and its receptor.

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