The comparison of three methods of drawing cerebrospinal fluid in rabbit

Yan, Li; Zhang, Bo; Wen, Wei; Liu, Shuang; Di, Hao; Liu, Ming; Kuang, Hai‐Xue; Huang, Shu-Ming

doi:10.1016/j.jneumeth.2012.06.018

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…Based on the published rabbit model of TBM using the lineage 2 Mtb strain HN878 ( 29 ), we initially infected nine rabbits intra-cisternally with 10 6 colony-forming units (CFUs) of an exponentially grown frozen HN878 culture. Inoculation was performed via puncture of the cisterna magna circumventing the use of a stereotaxic frame ( 28 ), similar to the method used for CSF collection ( 30 ). Thus, rabbits did not undergo any procedure (i.e., attaching an acrylic helmet and tightening metallic screws) prior to infection, thereby reducing stress and opportunities for secondary infection.…”

Section: Resultsmentioning

confidence: 99%

A preclinical model of TB meningitis to determine drug penetration and activity at the sites of disease

Lanni,

Antilus Sainte,

Hansen,

et al. 2023

Antimicrob Agents Chemother

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Tuberculosis meningitis (TBM) is essentially treated with the first-line regimen used against pulmonary tuberculosis, with a prolonged continuation phase. However, clinical outcomes are poor in comparison, for reasons that are only partially understood, highlighting the need for improved preclinical tools to measure drug distribution and activity at the site of disease. A predictive animal model of TBM would also be of great value to prioritize promising drug regimens to be tested in clinical trials, given the healthy state of the development pipeline for the first time in decades. Here, we report the optimization of a rabbit model of TBM disease induced via inoculation of Mycobacterium tuberculosis into the cisterna magna, recapitulating features typical of clinical TBM: neurological deterioration within months post-infection, acid-fast bacilli in necrotic lesions in the brain and spinal cord, and elevated lactate levels in cerebrospinal fluid (CSF). None of the infected rabbits recovered or controlled the disease. We used young adult rabbits, the size of which allows for spatial drug quantitation in critical compartments of the central nervous system that cannot be collected in clinical studies. To illustrate the translational value of the model, we report the penetration of linezolid from plasma into the CSF, meninges, anatomically distinct brain areas, cervical spine, and lumbar spine. Across animals, we measured the bacterial burden concomitant with neurological deterioration, offering a useful readout for drug efficacy studies. The model thus forms the basis for building a preclinical platform to identify improved regimens and inform clinical trial design.

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Section: Resultsmentioning

confidence: 99%

A preclinical model of TB meningitis to determine drug penetration and activity at the sites of disease

Lanni,

Antilus Sainte,

Hansen,

et al. 2023

Antimicrob Agents Chemother

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“…Rotating the probe 90° counter clockwise, corresponding transverse images were obtained. The ultrasonographic anatomy of the region was described in detail using an anatomy atlas, anatomic corrosion casts (Figure ), ultrasonography book, and peer‐reviewed scientific articles as references.…”

Section: Methodsmentioning

confidence: 99%

“…During cerebrospinal fluid sampling, it is important to avoid any blood contamination that could lead to misinterpretation of the results and, most important, to avoid iatrogenic injuries . To obtain a sample of cerebrospinal fluid, the puncture of the cisterna magna is a relatively common diagnostic procedure both in rabbit clinical medicine and in central nervous system research …”

Section: Introductionmentioning

confidence: 99%

“…4,5 To obtain a sample of cerebrospinal fluid, the puncture of the cisterna magna is a relatively common diagnostic procedure both in rabbit clinical medicine and in central nervous system research. 2,6 The cisterna magna (also referred as cerebellomedullary cistern) is the dorsal widening of the subarachnoid space, located between the occipital condyles and the atlas (atlanto-occipital region), in the angle formed by the caudal surface of the cerebellum and the dorsal aspect of the medulla oblongata. 7,8 The standard technique to obtain a cerebrospinal fluid sample is a blind puncture of the cisterna magna.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasonographic anatomy of the atlanto‐occipital region and ultrasound‐guided cerebrospinal fluid collection in rabbits (Oryctolagus cuniculus)

Anselmi

Dias

Martorell

et al. 2017

Vet Radiology Ultrasound

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Cerebrospinal fluid analyses are important for diagnosis of neurologic problems in rabbits and for translational research projects using rabbits as models. Blind puncture of the cisterna magna is the current standard technique for sampling cerebrospinal fluid in this species. However, the complexity and small size of the cisterna magna and surrounding structures are limitations of this technique. Aims of this prospective, anatomic, pilot study were to (1) describe the normal anatomy of the atlanto-occipital region, (2) describe ultrasonographic anatomic landmarks, and (3) develop and evaluate a technique for ultrasound-guided puncture of the cisterna magna for cerebrospinal fluid sampling in rabbits. Thirty healthy rabbits were included and the study was conducted in three stages. Three rabbit cadavers were used for the first stage of the study. Then, the second stage was completed using 13 rabbit cadavers. Finally, the third stage was completed in 14 live rabbits. The ultrasound-guided puncture performed in 13 cadavers was successful at the first attempt in 10 cases, and at the second attempt in the remaining three cases. In the in vivo study, the ultrasound-guided puncture was successful in all 14 cases, without signs of complications. Findings supported the use of ultrasound-guided puncture of the cisterna magna as a safe technique that may be used routinely or when the sample of cerebrospinal fluid cannot be obtained with the blind technique in rabbits.

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Rabbit Basic Science

Smith¹

2023

Textbook of Rabbit Medicine

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The comparison of three methods of drawing cerebrospinal fluid in rabbit

Cited by 7 publications

References 17 publications

A preclinical model of TB meningitis to determine drug penetration and activity at the sites of disease

A preclinical model of TB meningitis to determine drug penetration and activity at the sites of disease

Ultrasonographic anatomy of the atlanto‐occipital region and ultrasound‐guided cerebrospinal fluid collection in rabbits (Oryctolagus cuniculus)

Rabbit Basic Science

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