The comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats

Abstract

Recent studies have provided important evidence that neuroinflammation has an effective role in epilepsy pathophysiology. However, it is not clear that the occurrence of neuroinflammation is related to one epileptic seizure or repeating seizures. Therefore, we aimed to investigate the comparison of inflammatory markers in pentylenetetrazole-induced acute epileptic seizure model and chronic epilepsy model in rats. In this study, 18 male Wistar albino rats were used. The animals divided into three groups as control, acute epileptic seizure model and chronic epilepsy model. Inflammatory markers (TNF-α, IL-1 β, COX-1, and COX-2) were measured by using ELISA methods in the cortical and hippocampal brain regions after completing the epileptic model procedure. Statistical evaluation of the data was performed by one-way ANOVA and multiple comparisons were determined by the Tukey test. Statistical significance was defined at p<0.05. Obtained data show that there was significant increase in inflamattory markers in chronic epilepsy model compared to the control and acute epileptic seizure model (p<0.05). In conlusion, this study may suggest that inflammatory sytem is related to epileptogenesis process rather than only one epileptic seizure.

The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

“…In addition, as in our study, there may not be enough time to obtain an inflammatory response in an acute seizure model. Supporting this possibility, in a recent study, Taskıran et al compared inflammatory markers in the brain in PTZ-induced acute seizure and chronic epilepsy model and reported more significant results in the chronic PTZ model compared to acute seizure model [19]. Similar to IL-1β, pro-inflammatory cytokine TNF-α is also released from activated glial cells [20].…”