The comparison of glycosphingolipids isolated from an epithelial ovarian cancer cell line and a nontumorigenic epithelial ovarian cell line using MALDI-MS and MALDI-MS/MS

Rajanayake, Krishani K.; Taylor, William R.; Isailović, Dragan

doi:10.1016/j.carres.2016.05.006

Cited by 8 publications

(5 citation statements)

References 64 publications

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“…To minimize the possibility of errors, these programs are usually equipped with various filters to eliminate unwanted matches; some of them allow the use of rule-based identification. Below, are the most popular commercial and free available ones used for these purposes: Lipid View [186], Lipid Search [177], SimLipid [187], LipidXplorer [188], LipiDex [189], LIMSA [190], Lipidyzer [191], Lipid Data Analyzer [192], LipidQA [193], CEU Mass Mediator [194], LipidLama [195], LipidMatch [196], LipidMiner [197], dGOT-MS [198], ALEX [199], The Lipid Annotation Service (LAS) [200], and LOBSTAHS [201]. Thus, the data accumulated over many years make the non-targeted approach simpler, and therefore more popular among researchers.…”

Section: Programs and Toolsmentioning

confidence: 99%

Methods of the Analysis of Oxylipins in Biological Samples

et al. 2020

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Oxylipins are derivatives of polyunsaturated fatty acids and due to their important and diverse functions in the body, they have become a popular subject of studies. The main challenge for researchers is their low stability and often very low concentration in samples. Therefore, in recent years there have been developments in the extraction and analysis methods of oxylipins. New approaches in extraction methods were described in our previous review. In turn, the old analysis methods have been replaced by new approaches based on mass spectrometry (MS) coupled with liquid chromatography (LC) and gas chromatography (GC), and the best of these methods allow hundreds of oxylipins to be quantitatively identified. This review presents comparative and comprehensive information on the progress of various methods used by various authors to achieve the best results in the analysis of oxylipins in biological samples.

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Section: Programs and Toolsmentioning

confidence: 99%

Methods of the Analysis of Oxylipins in Biological Samples

et al. 2020

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“…Previous works on permethylated glycolipids have focused on structural analysis without any chromatographic separation. ,− For quantitative purposes, particularly with limited amount of samples, chromatography prior to MS detection offers a great advantage in terms of improving the sensitivity of analysis due to chromatographic focusing, and it also allows resolution of possible isobaric compounds. Reversed-phase column for separation prior to MS analysis is ideal for permethylated glycoconjugates owing to their increased hydrophobicity. , In this study, we used Cortecs C18 RPLC column.…”

Section: Results and Discussionmentioning

confidence: 99%

Differential Isotope Labeling by Permethylation and Reversed-Phase Liquid Chromatography–Mass Spectrometry for Relative Quantification of Intact Neutral Glycolipids in Mammalian Cells

Barrientos¹,

Zhang²

2019

Anal. Chem.

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Probing the role of glycolipids in health and disease warrants development of practical strategies to determine these molecules at the intact structural level, namely to simultaneously characterize and quantify the glycan and lipid moieties without breaking the linkage between them. Herein we present such an approach utilizing differential isotope labeling and reversed phase liquid chromatography–tandem mass spectrometry (RPLC–MS/MS) for structural characterization and relative quantification of intact neutral glycolipids. In this approach, each individual sample and a pooled aliquot of each sample were permethylated using 12CH3I and 13CH3I, respectively, with the latter one serving as internal reference standard. The individual 12C-permethylated samples were spiked with equal amounts of the 13C-permethylated pooled sample and analyzed by RPLC–MS/MS. Permethylation not only increased the ionization efficiency of glycolipids but also facilitated structural characterization of both moieties. The ratio of the peak areas between the 12C- and 13C-labeled glycolipids served as surrogate measure of their relative concentrations. The coefficient of variation of the method was <6% measured across four representative glycolipids in five different ratios and triplicate experiments, after correction of natural isotopic distribution. When analyzing the low abundant glycolipids in total lipid extract, permethylation can dramatically reduce the analytical background by depleting most of the highly abundant ester-linked lipids. Application to conduritol B epoxide-, a β-glucocerebrosidase inhibitor, treated RAW 264.7 cells demonstrated the practical utility of this method in profiling the temporal accumulation of different glycolipids. Overall, this methodology offers a practical LC–MS based identification and quantification strategy to advance intact glycolipids analysis in mammalian cells.

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“…Ovarian cancer is the seventh most common cancer and eighth most common cause of cancer-related death in women. Rajanayake et al (2016) compared GSL profiles in epithelial ovarian cancer SKOV3 cells vs. benign ovarian T29 cells. Five neutral GSLs were found only in SKOV3 cells, and several sialylated GSLs were differentially expressed.…”

Section: Prostate and Ovarian Cancermentioning

confidence: 99%

Biological Roles of Aberrantly Expressed Glycosphingolipids and Related Enzymes in Human Cancer Development and Progression

Zhuo

Guan

2018

Front. Physiol.

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Glycosphingolipids (GSLs), which consist of a hydrophobic ceramide backbone and a hydrophilic carbohydrate residue, are an important type of glycolipid expressed in surface membranes of all animal cells. GSLs play essential roles in maintenance of plasma membrane stability, in regulation of numerous cellular processes (including adhesion, proliferation, apoptosis, and recognition), and in modulation of signal transduction pathways. GSLs have traditionally been classified as ganglio-series, lacto-series, or globo-series on the basis of their diverse types of oligosaccharide chains. Structures and functions of specific GSLs are also determined by their oligosaccharide chains. Different cells and tissues show differential expression of GSLs, and changes in structures of GSL glycan moieties occur during development of numerous types of human cancer. Association of GSLs and/or related enzymes with initiation and progression of cancer has been documented in 100s of studies, and many such GSLs are useful markers or targets for cancer diagnosis or therapy. In this review, we summarize (i) recent studies on aberrant expression and distribution of GSLs in common human cancers (breast, lung, colorectal, melanoma, prostate, ovarian, leukemia, renal, bladder, gastric); (ii) biological functions of specific GSLs in these cancers.

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The comparison of glycosphingolipids isolated from an epithelial ovarian cancer cell line and a nontumorigenic epithelial ovarian cell line using MALDI-MS and MALDI-MS/MS

Cited by 8 publications

References 64 publications

Methods of the Analysis of Oxylipins in Biological Samples

Methods of the Analysis of Oxylipins in Biological Samples

Differential Isotope Labeling by Permethylation and Reversed-Phase Liquid Chromatography–Mass Spectrometry for Relative Quantification of Intact Neutral Glycolipids in Mammalian Cells

Biological Roles of Aberrantly Expressed Glycosphingolipids and Related Enzymes in Human Cancer Development and Progression

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