Abstract:High performance liquid chromatography (HPLC) pump has a main function to deliver a mobile phase which need accuracy and stable flow rate. Recently, three flow rate calibration methods are used to test the HPLC pump performance such as using standard flow meter, gravimetric method, and volumetric method. These calibration methods have not been clearly described. Therefore, this study aimed to investigate and compare the flow rate calibration by the using of standard flow meter, gravimetric method, and volumetr… Show more
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