The Comparison of Cellulolytic Activity of the Modified Soil Treated with Roundup

Abstract

The activity of soil microorganisms affects soil fertility and structure, what leads to satisfactory crop yields, thanks to changes in the decay of organic matter. Their activity may be impaired as a result of application by farmers both fertilizers and pesticides. The degradation of cellulosic biomass represents an important part of the carbon cycle within the biosphere. Cellulolitic microorganisms are responsible for this decomposition, as they produced the enzymes of the cellulase complex. The aim of the study was to compare the cellulolytic activity of soil with Roundup and modified using urea phosphate and / or manure. The pot experiment was conducted under laboratory conditions. The studied material was brown soil (pH of 5.5) fertilized with manure and/or urea phosphate. To each earthenware vase were fed 0.5 kg of the tested soil supplemented with 1% carboxymethyl cellulose. The cellulolytic activities of soil in different experimental variants were expressed in % of C using a colorimetric Petkov method. Modification of soil cellulolytic activity varied considerably depending on the additive manure and/or urea phosphate and Roundup. The lowest soil cellulolytic activity was observed in the presence of manure and urea phosphate, and the highest in the soil with manure and Roundup. The objects of Roundup independently of the other additives urea phosphate stimulate the activity of the cellulolytic microflora compared to objects containing only urea phosphate. The presence of manure in soil treated with Roundup clearly accelerated degradation of cellulose, what can be used in agriculture in the degradation of crop residues. The study shows that pesticides and fertilizers have a big impact on cellulolytic activity in soil. Changes in cellulolytic activity can be used as an ecological indicator of soil pollution level.

Screening for Hydrolytic Enzyme Production by Thermophilic Microbes Isolated from Egyptian Hot Spring

“…To determine their capacity to produce hydrolytic enzymes, bacterial and actinobacterial isolates were screened to evaluate lipase, amylase and A total twenty four isolates were subjected to enzyme screening, the majority of the isolates showed activity for cellulase. According to Krzyśko-Łupicka et (2016) cellulose is the principal mass of organic matter that is degraded by soil microorganisms completely or partially. Another factor can affect the activity of cellulase is pH of the sample.…”

Целюлозоруйнуюча Активність Чорноземних Ґрунтів Лівобережжя Лісостепу України

“…Цей фермент досить добре вивчений, але інтерес до нього не пропадає тому, що саме цьому ферменту і мікроорганізмам, що його синтезують відводиться головна роль у кругообігу вуглецю, а саме виділенню у атмосферу CO 2 . Тому целюлозоруйнуюча активність вивчається різними методами у різних ґрунтах і за різних умов [1][2][3][4][5][6]. Оскільки целюлаза безпосередньо впливає на швидкість розкладу рослинних решток, а целюлоза є домінуючим компонентом рослинних решток і органічних добрив, вивчення активності цього ензиму є першочерговою задачею при вивченні біологічної активності ґрунтів, а сам показник є чудовим біоіндикатором загального стану агроценозів [7,8].…”

Microbial transformation of soil organic matter under varying agricultural management systems in Ukraine

“…The activity of cellulases and the process of cellulose destruction in the soil of agro-ecosystems is determined by several factors, including temperature, pH of the environment, soil structure, chemical structure of OM and its distribution according to the soil profile, and the quality of introduced organic substances with fertilisers ( Choi et al, 2018 ). Consequently, considering these factors makes it possible to use cellulolytic activity as a sensitive test for assessing the state of the soil under the influence of various agricultural technologies and pollution by heavy metals ( Fan et al, 2012 ; Krzyśko-Łupicka et al, 2017 ). Therefore, this work aimed to investigate the content of OM in various soil types of Ukraine in natural and agricultural ecosystems and the processes of microbial destruction of cellulose biopolymer.…”