The comparison of biological effects of bacterial and synthetic melanins in neuroblastoma cells

Handl, Jiří; Majtnerová, Pavlína; Čapek, Jan; Česla, Petr; Hovsepyan, A. S.; Avetisyan, Sona; Micankova, Petra; Brůčková, Lenka; Staňková, Pavla; Knotkova, Katerina; Petrosyan, Tigran; Roušar, Tomáš

doi:10.1016/j.fct.2022.113355

Cited by 5 publications

(2 citation statements)

References 67 publications

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“…BM may promote the survival of neurons in the substantia nigra in the presence of toxic factors. In vitro studies have indicated the survival-promoting activity of bacterial melanin on midbrain dopaminergic neuron cultures [ 19 ]. BM has the potential to enhance the survival of neurons in SNc under the influence of toxic factors, suppress inflammatory processes, and stimulate regeneration processes in nervous tissue [ 15 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Regulatory effect of bacterial melanin on the isoforms of new superoxide-producing associates from rat tissues in rotenone-induced Parkinson’s disease

Danielyan,

Nebogova,

Simonyan

et al. 2023

BMC Neurosci

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According to recent research, selective neuronal vulnerability in Parkinson’s disease (PD) results from several phenotypic traits, including calcium-dependent, feed-forward control of mitochondrial respiration leading to elevated reactive oxygen species and cytosolic calcium concentration, an extensive axonal arbor, and a reactive neurotransmitter. Therefore, antioxidant therapy is a promising direction in the treatment of PD. In vitro studies have indicated the survival-promoting activity of bacterial melanin (BM) on midbrain dopaminergic neuron cultures. It has been established that BM has a number of protective and anti-inflammatory properties, so there is a high probability of a protective effect of BM in the early stages of PD. In this study, PD was induced through the unilateral intracerebral administration of rotenone followed by bacterial melanin. Tissues (brain, lungs, and small intestine) from the observed groups underwent isolation and purification to extract isoforms of new thermostable superoxide (О2−)-producing associates between NADPH-containing lipoprotein (NLP) and NADPH oxidase-Nox (NLP-Nox). The optical absorption spectral characteristics, specific amounts, stationary concentration of the produced О2−, and the content of NADPH in the observed associates were determined. The optical absorption spectra of the NLP-Nox isoforms in the visible and UV regions in the experimental groups did not differ from those of the control group. However, compared with the control group, the specific content of the total fractions of NLP-Nox isoforms associated with PD groups was higher, especially in the small intestine. These findings suggest that the described changes may represent a novel mechanism for rotenone-induced PD. Furthermore, bacterial melanin demonstrated antioxidant properties and regulated membrane formation in the brain, lung, and small intestine. This regulation occurred by inhibiting the release of new membrane-bound formations (NLP-Nox associates) from these membranes while simultaneously regulating the steady-state concentration of the formed О2−.

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Section: Introductionmentioning

confidence: 99%

Regulatory effect of bacterial melanin on the isoforms of new superoxide-producing associates from rat tissues in rotenone-induced Parkinson’s disease

Danielyan,

Nebogova,

Simonyan

et al. 2023

BMC Neurosci

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“…Moreover, foodborne diseases are widely caused by Enterobacteriaceae, which is highly spread among various aquatic systems to be colonized in humans, causing urinary tract infections. In addition, the abuse of antibiotics in the aquatic environment has led to the emergence of new bacterial Enterobacter strains [35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Elbahnaswy

Elshopakey

Shakweer

et al. 2023

Fishes

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Vibrio alginolyticus, Vibrio chlorea, and Enterococcus cloacae were the main bacterial pathogens investigated during recurrent episodes of mortalities among farmed flathead grey mullet (Mugil cephalus) in the Bahar El-Baqar area, Sharqia governorate, Egypt, during the summer season in 2021. Nevertheless, the contamination of water with heavy metals was evaluated. In this study, a total number of sixty moribund and freshly dead Mugil cephalus were examined clinically and upon post-mortem. Then, the bacterial isolates were subjected to bacteriological phenotypic examination, 16S rRNA gene sequencing, and antibiotic sensitivity testing. Histopathological screening and the immunity response of naturally infected M. cephalus were also implemented. In areas where bacterial strains were recovered in a high percentage, water contamination with Cu, Fe, and Ni was higher than the permissible limit. The results showed a high similarity (99–100%) to Vibrio and E. cloacae strains using phylogenetic analysis of 16S rRNA. Most isolates exhibited sensitivity to florfenicol, ciprofloxacin, and norfloxacin, while they were resistant to erythromycin and amoxicillin. Hemorrhage, oedema, hemosiderosis, and melanomacrophage activation in most affected tissues of infected M. cephalus were detected by light microscopy. Nevertheless, higher levels of AST and ALT, as well as serum inflammatory markers, CRP, and lysozyme activity, were shown in infected fish. There were higher MDA levels, along with a reduction of GSH, SOD, and catalase, in the gills and brain of infected M. cephalus, compared to the control fish. Our obtained data confirmed the high prevalence of Vibrio serovars and E. cloacae in infected grey mullets, describing their histopathological alterations, higher inflammatory response, and oxidative stress impact. These results could be useful indicators for understanding the pathogenesis of these pathogens in correlation with water parameter deterioration. Thus, the emergence of Vibrio and E. cloacae species in this study threatens the brackish water aquaculture industry in Egypt and poses a public health concern.

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Mitochondrial damage precedes the changes of glutathione metabolism in CdCl2 treated neuronal SH-SY5Y cells

Nyvltova,

Capek,

Handl

et al. 2024

Food and Chemical Toxicology

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The comparison of biological effects of bacterial and synthetic melanins in neuroblastoma cells

Cited by 5 publications

References 67 publications

Regulatory effect of bacterial melanin on the isoforms of new superoxide-producing associates from rat tissues in rotenone-induced Parkinson’s disease

Regulatory effect of bacterial melanin on the isoforms of new superoxide-producing associates from rat tissues in rotenone-induced Parkinson’s disease

Bacterial Co-Infection as a Potential Threat to Farmed Flathead Grey Mullet (Mugil cephalus): Phenotypic and Molecular Diagnosis, Histopathology, Immunity Response, and In Vitro Antibacterial Evaluation

Mitochondrial damage precedes the changes of glutathione metabolism in CdCl2 treated neuronal SH-SY5Y cells

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