The Comparison of a New Ultrasound-Induced Depression Model to the Chronic Mild Stress Paradigm

Zorkina, Yana; Зубков, Э. А.; Morozova, Anastasia; Ushakova, Valeria; Chekhonin, V. P.

doi:10.3389/fnbeh.2019.00146

Cited by 18 publications

(31 citation statements)

References 30 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rat pups emit 40 kHz signals when isolated from dams ( Takahashi et al, 2010 ). Unavoidable exposure of adult rats to US variable frequencies (22–40 kHz) induces a state of stress in them, and when chronically exposed for 3 weeks, the animals develop a depressive-like state, as previously demonstrated ( Morozova et al, 2013a , b , 2016 ; Zorkina et al, 2019 ). Therefore, we hypothesized that chronic exposure of pregnant females to variable US throughout the gestation period (21 days) would cause a stress response in them, which in turn would lead to developmental disorders in the offspring, including abnormalities in nervous system development and behavior.…”

Section: Introductionsupporting

confidence: 68%

“…The arena was cleaned after each animal. The animal was placed in a certain corner of the arena at the beginning of testing and its behavior is recorded over 5 min ( Zorkina et al, 2019 ). The following parameters were recorded: arena center crossing frequency, horizontal activity (total squares crossed), vertical activity (rearing frequency), the total time of freezing (s), the grooming total time (s), grooming frequency, and mean grooming duration (s).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Behavior and Postnatal Development in Infant and Juvenile Rats After Ultrasound-Induced Chronic Prenatal Stress

et al. 2021

View full text Add to dashboard Cite

Fetal development is susceptible to environmental factors. One such factor is exposure to stress during pregnancy. The present study aimed to investigate the effects of chronic prenatal stress (PS) on the development and behavior of rat offspring during infancy and juvenile ages. Existing approaches to modeling prenatal stress on animals do not correlate with the main type of stress in pregnant women, namely psychological stress. We used a new stress paradigm in the experiment, namely, stress induced by exposure to variable frequency ultrasound (US), which acted on pregnant Wistar rats on gestational days 1–21. This type of stress in rodents can be comparable to psychological stress in humans. We assessed physical development, reflex maturation, motor ability development, anxious behavior, response to social novelty, and social play behavior in male and female offspring. Additionally, we investigated maternal behavior and the effect of neonatal handling (NH) on behavior. Prenatal stress did not affect postnatal developmental characteristics in rat pups, but prenatally stressed rats had higher body weight in early and adult age than controls. Prenatal exposure to a stressor increased anxiety in the open-field test (OF), changed social preferences in the social novelty test (SN), and impaired social play behavior in males. Neonatal handling reduced anxiety and restored social behavior, but evoked hyperactive behavior in rat pups. Maternal behavior did not change. Our study demonstrated for the first time that exposure to variable frequency ultrasound during pregnancy influences offspring development and impairs behavior, correlating with the effects of other types of stress during pregnancy in rodents. This supports the idea of using this exposure to model prenatal stress.

show abstract

Section: Introductionsupporting

confidence: 68%

Section: Methodsmentioning

confidence: 99%

The Behavior and Postnatal Development in Infant and Juvenile Rats After Ultrasound-Induced Chronic Prenatal Stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Brain is more prone to excessive exercise injury compared to other organs (Liu et al, 2019), and it therefore might be necessary to evaluate the brain functions in excessive training. Morris water maze was a commonly used system for testing recognition, memory, and learning in animals (Zorkina et al, 2019). It can be used in combination with forced swimming protocols.…”

Section: Discussionmentioning

confidence: 99%

Effects of Mongolian Warm Acupuncture on iNOS/NO and Inflammatory Cytokines in the Hippocampus of Chronic Fatigue Rats

Shui

et al. 2020

Front. Integr. Neurosci.

View full text Add to dashboard Cite

The inducible nitric oxide synthase/nitric oxide (iNOS/NO) signaling pathway and inflammatory cytokines play important roles in the pathogenesis of exercise-induced fatigue. Studies have found that Mongolian warm acupuncture (WA) could alleviate exercise-induced fatigue. However, the exact mechanisms underlying its effects remain unclear. In the present study, we investigated the effects of Mongolian WA on iNOS/NO signaling pathway and proinflammatory cytokines in a chronic exhaustive swimminginduced fatigue rat model. Animals were randomly divided into Control group, Ctrl + WA group, Model group, and Model + WA group. The body weight, exhaustive swimming time test, and Morris water maze test were performed before and after the chronic exhaustive swimming. The serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and iNOS were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expressions of IL-1β, IL-6, TNF-α, IFN-γ, and iNOS in the hippocampus were measured by real-time polymerase chain reaction (RT-PCR). Moreover, the protein expression of iNOS in the hippocampus was measured by western blot, and the NO productions in the serum and hippocampus were detected by Griess reaction system. Chronic exhaustive exercise significantly reduced the body weight and exhaustive swimming time, and induced impairment in learning and memory, and which were reversed by WA treatment. Chronic exhaustive exercise also increased the expressions of iNOS and proinflammatory cytokines, while WA treatment significantly decreased the level of iNOS and proinflammatory cytokines. However, chronic exhaustive exercise did not affect the NO production. These findings demonstrated that WA could alleviate the chronic exhaustive swimming-induced fatigue and improve the learning and memory ability, and the actions might be related to the reduction of inflammatory response and iNOS expression.

show abstract

“…Dysregulation of neurotransmission underlies the number of brain diseases, making the biosensors useful in various animal models of human neuropsychiatric disorders. The animal models of human CNS disorders proved their effectiveness in studies of Parkinsonism (Blesa and Przedborski, 2014), major depressive disorder (Morozova et al, 2016; Zorkina et al, 2019), autism (Chesselet, 2005) and many others reviewed elsewhere (Keifer and Summers, 2016).…”

Section: Application Of Neurotransmitter Biosensors In Vivomentioning

confidence: 99%

Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications

Leopold

Shcherbakova

Verkhusha

2019

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Understanding how neuronal activity patterns in the brain correlate with complex behavior is one of the primary goals of modern neuroscience. Chemical transmission is the major way of communication between neurons, however, traditional methods of detection of neurotransmitter and neuromodulator transients in mammalian brain lack spatiotemporal precision. Modern fluorescent biosensors for neurotransmitters and neuromodulators allow monitoring chemical transmission in vivo with millisecond precision and single cell resolution. Changes in the fluorescent biosensor brightness occur upon neurotransmitter binding and can be detected using fiber photometry, stationary microscopy and miniaturized head-mounted microscopes. Biosensors can be expressed in the animal brain using adeno-associated viral vectors, and their cell-specific expression can be achieved with Cre-recombinase expressing animals. Although initially fluorescent biosensors for chemical transmission were represented by glutamate biosensors, nowadays biosensors for GABA, acetylcholine, glycine, norepinephrine, and dopamine are available as well. In this review, we overview functioning principles of existing intensiometric and ratiometric biosensors and provide brief insight into the variety of neurotransmitter-binding proteins from bacteria, plants, and eukaryotes including G-protein coupled receptors, which may serve as neurotransmitter-binding scaffolds. We next describe a workflow for development of neurotransmitter and neuromodulator biosensors. We then discuss advanced setups for functional imaging of neurotransmitter transients in the brain of awake freely moving animals. We conclude by providing application examples of biosensors for the studies of complex behavior with the single-neuron precision.

show abstract

The Comparison of a New Ultrasound-Induced Depression Model to the Chronic Mild Stress Paradigm

Cited by 18 publications

References 30 publications

The Behavior and Postnatal Development in Infant and Juvenile Rats After Ultrasound-Induced Chronic Prenatal Stress

The Behavior and Postnatal Development in Infant and Juvenile Rats After Ultrasound-Induced Chronic Prenatal Stress

Effects of Mongolian Warm Acupuncture on iNOS/NO and Inflammatory Cytokines in the Hippocampus of Chronic Fatigue Rats

Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications

Contact Info

Product

Resources

About