The comparison genomics analysis with glioblastoma multiforme (GBM) cells under 3D and 2D cell culture conditions

Zhang, Bin; Zhou, Changchun; Li, Yuting; Li, Binjie; Yu, Mengfei; Luo, Yichen; Gao, Lei; Zhang, Duo; Xue, Qingwu; Qiu, Qingchong; Lin, Biaoyang; Zou, Jun; Yang, Huayong

doi:10.1016/j.colsurfb.2018.09.034

Cited by 27 publications

(23 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several RNA-sequencing studies demonstrated significant, tumor-relevant molecular changes induced by 3D cell culture (12,22,23). Glioblastoma spheroids in 3D culture mimicked in vivo tissues, organs and even patient tumors, providing in vivo 34: 199-211 (2020) 204 similar cellular properties including hypoxic gradients, cellular heterogeneity with both slow-dividing cells and proliferative tumor bulk (24,25) and higher drug resistance (10).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several investigators have examined the effects of glioblastoma multiforme tumor cells culture condition on gene expression profiles. They found that in 3D glioblastoma multiforme PLA porous scaffolds compared to the 2D condition, genes in peroxisome proliferator-activated receptors and phosphatidylinositol 3'-kinase(PI3K)-AKT signaling pathways were up-regulated, while those in metabolism, or related to the extracellular matrix and transforming growth factor-beta pathways were down-regulated (12). Tejero, et al found that the 3D glioblastoma organoids promoted the appearance of a quiescent cell population that represented the self-renewal ability high therapy resistance, and mesenchymal gene signatures (13).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic Profiling of 3D Glioblastoma Tumoroids for the Identification of Mechanisms Involved in Anticancer Drug Resistance

Chaicharoenaudomrung

Kunhorm

Promjantuek

et al. 2019

In Vivo

View full text Add to dashboard Cite

Background/Aim: Among various types of brain tumors, glioblastoma is the most malignant and highly aggressive brain tumor that possesses a high resistance against anticancer drugs. To understand the underlined mechanisms of tumor drug resistance, a new and more effective research approach is required. The three dimensional (3D) in vitro cell culture models could be a potential approach to study cancer features and biology, as well as screen for anticancer agents due to the close mimicry of the 3D tumor microenvironments. Materials and Methods: With our developed 3D alginate scaffolds, Ilumina RNA-sequencing was used to transcriptomically analyze and compare the gene expression profiles between glioblastoma cells in traditional 2-dimensional (2D) monolayer and in 3D Ca-alginate scaffolds at day 14. To verify the reliability and accuracy of Illumina RNA-Sequencing data, ATP-binding cassette transporter genes were chosen for quantitative real-time polymerase chain reaction) verification. Results: The results showed that 7,411 and 3,915 genes of the 3D glioblastoma were up-regulated and down-regulated, respectively, compared with the 2D-cultured glioblastoma. Furthermore, the Kyoto Encyclopaedia of Genes and Genomes pathway analysis revealed that genes related to the cell cycle and DNA replication were enriched in the group of down-regulated gene. On the other hand, the genes involved in mitogen-activated protein kinase signaling, autophagy, drug metabolism through cytochrome P450, and ATP-binding cassette transporter were found in the up-regulated gene collection. Conclusion: 3D glioblastoma tumoroids might potentially serve as a powerful platform for exploring glioblastoma biology. They can also be valuable in anti-glioblastoma drug screening, as well as the identification of novel molecular targets in clinical treatment of human glioblastoma.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Transcriptomic Profiling of 3D Glioblastoma Tumoroids for the Identification of Mechanisms Involved in Anticancer Drug Resistance

Chaicharoenaudomrung

Kunhorm

Promjantuek

et al. 2019

In Vivo

View full text Add to dashboard Cite

show abstract

“…Recently, the genome of 3D glioblastoma multiforme (GBM) cells in polylactic acid porous scaffolds were compared to the genome of GBM cells in 2D cell culture conditions. It was found that the 14-d 3D GBM cells upregulated 8117 genes and downregulated 3060 genes compared to the 2D cell culture conditions[87]. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that genes involved in PPAR and PI3K-Akt signaling pathways were mainly upregulated, while genes involved in metabolism, ECM receptors, and transforming growth factor β pathway were downregulated.…”

Section: Application Of 3d Cell Culturementioning

confidence: 99%

Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling

Chaicharoenaudomrung

Kunhorm

Noisa

2019

WJSC

291

216

View full text Add to dashboard Cite

Three-dimensional (3D) culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures. In cancer and stem cell research, the natural cell characteristics and architectures are closely mimicked by the 3D cell models. Thus, the 3D cell cultures are promising and suitable systems for various proposes, ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives. This review provides a comprehensive compendium of recent advancements in culturing cells, in particular cancer and stem cells, using 3D culture techniques. The major approaches highlighted here include cell spheroids, hydrogel embedding, bioreactors, scaffolds, and bioprinting. In addition, the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed, and the prominent studies of 3D cell culture systems were discussed.

show abstract

“…The development of 3D in vitro tissue-engineered models will help to refine the drug response and contribute to the improvement of anti-cancer therapies. More recently, striking differences in gene expression between 2D and 3D culture of GBM have been reported (Ma et al, 2018) which mirrored the phenotypic differences. This is in agreement with another study (Chaicharoenaudomrung et al, 2020) where GBM cells were cultured in Ca-alginate 3D scaffolds before next-generation sequencing (Illumina), and this uncovered cellular pathways (Map kinase, autophagy, and cell metabolism) for 3D different to 2D cultures.…”

Section: Tissue-engineered Blood Vesselsmentioning

confidence: 80%

The Normal and Brain Tumor Vasculature: Morphological and Functional Characteristics and Therapeutic Targeting

et al. 2021

View full text Add to dashboard Cite

Glioblastoma is among the most common tumor of the central nervous system in adults. Overall survival has not significantly improved over the last decade, even with optimizing standard therapeutic care including extent of resection and radio- and chemotherapy. In this article, we review features of the brain vasculature found in healthy cerebral tissue and in glioblastoma. Brain vessels are of various sizes and composed of several vascular cell types. Non-vascular cells such as astrocytes or microglia also interact with the vasculature and play important roles. We also discuss in vitro engineered artificial blood vessels which may represent useful models for better understanding the tumor–vessel interaction. Finally, we summarize results from clinical trials with anti-angiogenic therapy alone or in combination, and discuss the value of these approaches for targeting glioblastoma.

show abstract

The comparison genomics analysis with glioblastoma multiforme (GBM) cells under 3D and 2D cell culture conditions

Cited by 27 publications

References 33 publications

Transcriptomic Profiling of 3D Glioblastoma Tumoroids for the Identification of Mechanisms Involved in Anticancer Drug Resistance

Transcriptomic Profiling of 3D Glioblastoma Tumoroids for the Identification of Mechanisms Involved in Anticancer Drug Resistance

Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling

The Normal and Brain Tumor Vasculature: Morphological and Functional Characteristics and Therapeutic Targeting

Contact Info

Product

Resources

About