2015
DOI: 10.1155/2015/458624
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The Comparative Utility of Viromer RED and Lipofectamine for Transient Gene Introduction into Glial Cells

Abstract: The introduction of genes into glial cells for mechanistic studies of cell function and as a therapeutic for gene delivery is an expanding field. Though viral vector based systems do exhibit good delivery efficiency and long-term production of the transgene, the need for transient gene expression, broad and rapid gene setup methodologies, and safety concerns regarding in vivo application still incentivize research into the use of nonviral gene delivery methods. In the current study, aviral gene delivery vector… Show more

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Cited by 22 publications
(15 citation statements)
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“…Before transfection, rat Schwann cells were plated on poly-L-lysine-coated plates and maintained until reaching 70% confluence. Transient transfections with WT-PMP22-Myc plasmids (Johnson et al, 2005) and scrambled or pooled rat PMP22 shRNA plasmids containing GFP reporters (Applied Biological Materials) were performed using lipofectamine 3000 (Invitrogen) in Opti-MEM medium (Thermo Fisher Scientific) (Rao et al, 2015). The sequences (from 5Ј-3Ј) of PMP22 shRNA are as follows: 40 GCGGT GCTAGTGTTGCTCTTCGTCTCCAC; 161 ACTCCTCATCTGTGAG CGAATGGCTTCAG; 313 CTTGCTGGTCTGTGTGTGATGAGTGC AGC; and 438 CCTCCTTAGTGGCATCATCTACGTGATCC.…”
Section: Methodsmentioning
confidence: 99%
“…Before transfection, rat Schwann cells were plated on poly-L-lysine-coated plates and maintained until reaching 70% confluence. Transient transfections with WT-PMP22-Myc plasmids (Johnson et al, 2005) and scrambled or pooled rat PMP22 shRNA plasmids containing GFP reporters (Applied Biological Materials) were performed using lipofectamine 3000 (Invitrogen) in Opti-MEM medium (Thermo Fisher Scientific) (Rao et al, 2015). The sequences (from 5Ј-3Ј) of PMP22 shRNA are as follows: 40 GCGGT GCTAGTGTTGCTCTTCGTCTCCAC; 161 ACTCCTCATCTGTGAG CGAATGGCTTCAG; 313 CTTGCTGGTCTGTGTGTGATGAGTGC AGC; and 438 CCTCCTTAGTGGCATCATCTACGTGATCC.…”
Section: Methodsmentioning
confidence: 99%
“…Cationic polyethylenimine (PEI)-DNA ‘polyplexes’ are avidly used for gene delivery (Goula et al, 1998; Rao et al, 2015). Polyamidoamines (PAMAM) and poly(lactic-co-glycolic) acid (PLGA) have also been frequently examined.…”
Section: Delivery Systemsmentioning
confidence: 99%
“…Lipid containing substances are frequently used for CNS delivery (Rao et al, 2015). Early investigations showed liposome-mediated gene delivery, i.e.…”
Section: Delivery Systemsmentioning
confidence: 99%
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“…However, macrophages, including microglia, are equipped with a series of cytoplasmic enzymes that degrade foreign nucleic acids, which results in low transfection efficiencies with the currently available chemical based techniques [10]. Indeed, while the cationic lipid-based transfection reagent Lipofectamine is mostly used for introducing foreign DNA in BV2 cells [11,12] the amount of viable transfected cells is considerably low [13]. Despite the benefit of gene delivery in BV2 cells, a comparative analysis of chemical transfection methods is lacking.…”
Section: Introductionmentioning
confidence: 99%