The comparative evaluation of biofilm production and antibiotic resistance in Pseudomonas aeruginosa isolates

Abstract

In this study, it was aimed to investigate the biofilm formation, antibiotic resistance profiles, the genes which play roles for biofilm and antibiotic resistances of 48 Pseudomonas aeruginosa isolates isolated from different clinical samples. The identifications of the P.aeruginosa isolates were confirmed with PCR. The antibiotic resistances against 8 antibiotics were determined by Kirby-Bauer Disc Diffusion method. The biofilm formations of the isolates were investigated in vitro with Congo Red Agar, Modified Tube Adherence (Christensen) and Micro plate Methods. The isolates which were found as positive with three methods were genotyped with RAPD-PCR. pelA, lasI, lasR, rhlI, rhlR,PA0756, PA0757, PA2070 and PA5033 genes were investigated with using PCR. The antibiotic resistance rates of the isolates among enrofloxacine, ceftriaxone, amoxicilline-clavulanic acid, ciprofloxacin, meropenem, colistin, gentamycin, and azithromycin were found as 16.67%, 60.42%, 100%, 10.42%, 29.17%, 4.17%, 12.5% and %97.9, respectively. The biofilm formation ratios were determined as 83,33% with CRA, 93.75% with Christensen method and 64.58% with micro plate method. pelA, lasI, rhlI and rhlR were carried by all the isolates. lasR gene was found in %97.92 of the isolates. Fortysix (95.83%) of the isolates carried PA0756 and PA0757. Fortyfive (93.75%) of the isolates were found as positive for PA2070 and PA5033 genes. The phylogenetic relations of the biofilm positive isolates were calculated as 56–93%. The biofilm formation and multiple antibiotic resistance rates were found as high in the clinical isolates of P. aeruginosa. The widespread and advanced studies thought to be performed for the lightening of this situation and biofilm related antibiotic resistances.