The Comparative Cryobiology of Preimplantation Embryos from Domestic Animals

Mullen, Steven F.; Critser, John K.

doi:10.1002/9780470390290.ch9

Cited by 2 publications

(2 citation statements)

References 214 publications

(171 reference statements)

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“…Khosla and co-workers microinjected propylene glycol into zebrafish embryos along with GNRs, followed by vitrification in liquid nitrogen. They demonstrated the ability to thaw the zebrafish embryos rapidly (1.4 × 10 7 ºC/min) by irradiating the sample with a 1,064 nm laser pulse for 1 ms (Khosla, Wang, TA B L E 4 Comparison between vitrification and slow-cooling cryopreservation methods (Almiñana & Cuello, 2015;Assumpção et al, 2008;Massip, 2001;Massip & Leibo, 2002;Moore & Bonilla, 2007;Mullen & Critser, 2008;Sanches, Zangirolamo, Silva, Morotti, & Seneda, 2017;Vajta & Nagy, 2006) The term 'full verification of solute penetration and dehydration rate' refers to the possibility of following these events under the stereomicroscope to the full extent (and in the case of slow freezing until 'seeding') and verifying/confirming the cryo steps through embryo shrinking and expansion. Hagedorn, Qin, & Bischof, 2017).…”

Section: Future Cons Ider Ationsmentioning

confidence: 99%

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Ferré

Kjelland

Taiyeb³

et al. 2020

Reprod Domestic Animals

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Contents Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high‐merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra‐low temperature storage method in order to maximize donor ovum pickup (OPU) turn‐over, recipient availability/utilization and domestic/overseas commercial trading opportunities. However, IVP bovine embryos are still very sensitive to chilling and cryopreservation, and despite recent progress, a convenient (simple and robust) protocol has not yet been developed. At the moment, there are two methods for bovine IVP embryo cryopreservation: slow programmable freezing and vitrification. Both of the aforementioned techniques have pros and cons. While controlled‐rate slow cooling can easily be adapted for direct transfer (DT), ice crystal formation remains an issue. On the other hand, vitrification solved this problem but the possibility of successful DT commercial incorporation remains to be determined. Moreover, simplification of the vitrification protocol (including warming) through the use of an in‐straw dilution without the use of a microscope is a prerequisite for its use under farm conditions. This review summarizes the bovine IVP embryo cryopreservation achievements, strengths and limitations of both freezing systems and prospective improvements to enhance cryosurvival, as well as perspectives on future directions of this assisted reproductive technology.

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Section: Future Cons Ider Ationsmentioning

confidence: 99%

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Ferré

Kjelland

Taiyeb³

et al. 2020

Reprod Domestic Animals

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“…After the initial report in 1985, numerous experiments describing successful vitrification of embryos from other species were published. It is beyond the scope of the present work to describe these reports in detail, but such information can be obtained in recently published reviews [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Vitrification: Fundamental Principles and Its Application for Cryopreservation of Human Reproductive Cells

Schiewe¹,

Mullen²

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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The fundamental understanding of cryobiology through experimentation in the 1960s, 1970s, and 1980s has led to the development of today's vitrification technology. Although human embryo and oocyte vitrification was slow to evolve, it has become an invaluable technology in the field of reproductive medicine. The aim of this chapter is to discuss some of the underlying basic principles behind forming a metastable glass phase during rapid cooling in liquid nitrogen (LN 2) and the prevention of recrystallization events upon warming. We then highlight how this understanding has led to its highly effective and reliable usage in clinical IVF. Furthermore, we describe how quality control factors (e.g., ease of use, repeatability, reliability, labeling security, and cryostorage safety) can vary between vitrification device systems, potentially influencing clinical outcomes and creating possible liability issues. An open-minded approach to continued experimentation is a necessity, especially pertaining to oocyte freeze preservation, if we are to optimize the vitrification of reproductive cells and tissue in the future.

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The Comparative Cryobiology of Preimplantation Embryos from Domestic Animals

Cited by 2 publications

References 214 publications

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Vitrification: Fundamental Principles and Its Application for Cryopreservation of Human Reproductive Cells

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