The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes

Luraschi, Roberto; Barrera-Avalos, Carlos; Vallejos‐Vidal, Eva; Alarcón, Javiera; Mella-Torres, Andrea; Hernández, Felipe; Inostroza-Molina, Ailen; Valdés, Daniel; Imarai, Mónica; Acuña-Castillo, Claudio; Reyes-López, Felipe E.; Sandino, Ana María

doi:10.1155/2022/2594564

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…The detection of SARS-CoV-2 was carried out using the ORF1ab gene probe from TaqMan TM 2019nCoV Assay Kit v1 (Thermo Fisher Scientific, Reference code. A47532) as previously reported by our group (26). We included samples with matching identification numbers to accurately identify RT-qPCR tests from the same patient across different periods.…”

Section: Study Design Sample Collection and Covid-diagnosismentioning

confidence: 99%

An ecological study on reinfection rates using a large dataset of RT-qPCR tests for SARS-CoV-2 in Santiago of Chile

Acuña-Castillo

Barrera-Avalos

Bachelet

et al. 2023

Front. Public Health

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IntroductionAs the SARS-CoV-2 continues to evolve, new variants pose a significant threat by potentially overriding the immunity conferred by vaccination and natural infection. This scenario can lead to an upswing in reinfections, amplified baseline epidemic activity, and localized outbreaks. In various global regions, estimates of breakthrough cases associated with the currently circulating viral variants, such as Omicron, have been reported. Nonetheless, specific data on the reinfection rate in Chile still needs to be included.MethodsOur study has focused on estimating COVID-19 reinfections per wave based on a sample of 578,670 RT-qPCR tests conducted at the University of Santiago of Chile (USACH) from April 2020 to July 2022, encompassing 345,997 individuals.ResultsThe analysis reveals that the highest rate of reinfections transpired during the fourth and fifth COVID-19 waves, primarily driven by the Omicron variant. These findings hold despite 80% of the Chilean population receiving complete vaccination under the primary scheme and 60% receiving at least one booster dose. On average, the interval between initial infection and reinfection was found to be 372 days. Interestingly, reinfection incidence was higher in women aged between 30 and 55. Additionally, the viral load during the second infection episode was lower, likely attributed to Chile's high vaccination rate.DiscussionThis study demonstrates that the Omicron variant is behind Chile's highest number of reinfection cases, underscoring its potential for immune evasion. This vital epidemiological information contributes to developing and implementing effective public health policies.

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Section: Study Design Sample Collection and Covid-diagnosismentioning

confidence: 99%

An ecological study on reinfection rates using a large dataset of RT-qPCR tests for SARS-CoV-2 in Santiago of Chile

Acuña-Castillo

Barrera-Avalos

Bachelet

et al. 2023

Front. Public Health

Self Cite

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Expression of Concern on “The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes”

Microbiology¹

2023

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Can circulating oxidative stress-related biomarkers be used as an early prognostic marker for COVID-19?

Uysal

Yüksel²,

Durmuş³

et al. 2023

Front. Med.

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BackgroundOxidative stress plays an important role in the pathogenesis of many diseases. This study aimed to investigate the relationship between nuclear factor kappa B (NF-κB) and oxidative stress and the severity of the disease in new COVID-19 patients, and, to compare the levels of NF-κB, oxidized LDL (oxLDL), and lectin-like oxidized-LDL receptor-1 (LOX-1) with oxygen saturation, which is an indicator of the severity parameters of the disease in COVID-19 patients.MethodsIn this prospective study, 100 COVID-19 patients and 100 healthy subjects were selected.ResultsLOX-1, NF-κB, and oxLDL were found to be higher in COVID-19 patients compared to the healthy subjects (p < 0.001 for all). According to the results of correlation analysis, it was found that there was no significant relationship between oxygen saturation and LOX-1, NF-κB and oxLDL parameters. There was significant relationship between oxLDL with LOX-1 and NF-κB in patients with COVID-19 disease. ROC analysis results of the highest discrimination power were oxLDL (AUC: 0.955, CI: 0.904–1.000; sensitivity: 77%, and specificity: 100%, for cutoff: 127.944 ng/l) indicating COVID-19.ConclusionOxidative stress plays an essential role in COVID-19. NF-κB, oxLDL, and LOX-1 seem to represent good markers in COVID-19. Our study also showed that oxLDL has the highest power in distinguishing patients with COVID-19 from the healthy subjects.

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The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes

Cited by 4 publications

References 20 publications

An ecological study on reinfection rates using a large dataset of RT-qPCR tests for SARS-CoV-2 in Santiago of Chile

An ecological study on reinfection rates using a large dataset of RT-qPCR tests for SARS-CoV-2 in Santiago of Chile

Expression of Concern on “The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal Genes”

Can circulating oxidative stress-related biomarkers be used as an early prognostic marker for COVID-19?

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