The comparative analysis of homologous fresh frozen bone and autogenous bone graft, associated or not with autogenous bone marrow, in rabbit calvaria: a clinical and histomorphometric study

Abstract

The aim of this study was to evaluate the potential of fresh frozen homologous and autogenous grafts, associated or not with autogenous bone marrow, to form bone. Sixty titanium cylinders were used, and were fixed to the skulls of 30 rabbits. These cylinders were filled with (A) autogenous bone (AM) autogenous bone associated with the bone marrow (H) fresh frozen homologous bone (HM) fresh frozen homologous bone associated with the bone marrow (M) pure autogenous bone marrow and (C) blood clot. The animals were sacrificed after 02 and 03 months. After clinical evaluation, the samples were stained with hematoxylin, eosin and Mallory Trichrome dyes for optical microscopy analysis and histomorphometric analysis. Experimental groups that received mineralized materials (A, AM, H, HM) showed the best bone formation results, presenting no statistical difference between them (P > 0.05). Groups that did not receive mineralized materials (M and C) showed the worst results (P < 0.05), but the M group showed better results than the C group. Most of the autogenous and homologous bone particles were resorbed and there was a larger amount of residual particles in the homologous graft (H, HM) when compared with the autogenous graft (A, AM; P < 0.05). These findings suggest that fresh frozen homologous grafts produced similar amounts of new bone when compared with the autogenous grafts. However, the amount of residual bone particles was larger in the homogenous groups, which may indicate a slower remodeling process. The homologous fresh frozen bone seems to be a good osteoconductive material. The use of only autogenous bone marrow showed better results when compared to the bood clot. However, this research indicates that association with mineralized materials is required.

Prosthetic Rehabilitation Using Short Dental Implants Associated With Homogenous Bone Graft

“…Moreover, with regard to longterm osseointegration, the homogenous material presented a satisfactory result, similar to that of autogenous bone. 15 Previously, according to some authors, short implants presented greater risk of failures when compared with the long type. However, more recent studies have related that the use of short implants alone presents a success rate of 84% to 98.7%.…”

The Use of Computer-Guided Flapless Dental Implant Surgery (NobelGuide) and Immediate Function to Support a Fixed Full-ArchProsthesis inFresh-Frozen Homologous Patients with Bone Grafts

“…38 Fresh-frozen homologous bone grafts resulted in volumes of bone similar to those obtained with autologous bone grafts; however, the amount of residual bone particles seems to be greater, which may indicate a slower remodeling process. 39 The behavior of FFB when used in combination with computerguided implant surgery is still not well understood because studies are absent in the literature.…”

Presence of Cells in Fresh-Frozen Allogeneic Bone Grafts from Different Tissue Banks

“…It is therefore considered the first treatment option for bone reconstruction (5). Such an approach is however riddled with morbidity, including postoperative pain at the donor site, not to mention the limited availability of bone tissue, which may hinder its applicability in most cases (6).…”