The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota

Krismer, Bernhard; Weidenmaier, Christopher; Zipperer, Alexander; Peschel, Andreas

doi:10.1038/nrmicro.2017.104

Cited by 265 publications

(226 citation statements)

References 158 publications

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“…На основании этих наблюдений можно выдвинуть предположение об отсутствии антагонизма между изучаемыми микроорганизмами. В то же время имеются данные о существовании естественного антагонизма между представителями нор-мальной микробиоты и потенциально патогенными микроорганизмами (патобионтами) [19]. Носительство патобионтов можно расценивать как дисбиоз, развивающийся на фоне нарушения колонизационной резистентности.…”

Section: Discussionunclassified

Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

Комбарова

Бичучер

Soldatskiĭ

et al. 2020

Russian Journal of Infection and Immunity

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Meningococcal, pneumococcal, streptococcal A and Haemophilus influenzae infections are manifested in different clinical forms, ranging from bacterial carriage to generalized life-threatening conditions. However, a connection between bacterial carriage and disease development has not been fully explored. A PCR assay was performed with adenoid biopsy samples collected from 112 children after planned adenotomy to detect Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, H. influenzae carriage. A DNA specific to at least one of the four studied microbial species was found in 104 samples (92.86%) so that: meningococcal DNA was detected in one sample (0.9%), pneumococcal — in 98 (87.5%), H. influenzae — in 19 (16.96%), and streptococcal A — in 42 (37.5%) samples. However, none of these species was found in 8 children (7.14%). A sole S. pneumoniae was detected in 54 samples (48.2%), whereas S. pyogenes — in 5 samples (4.5%). Moreover, two bacterial species were simultaneously as follows: N. meningitidis and S. pneumoniae — in 1 sample (0.9%), S. pneumoniae and H. influenzae — in 7 samples (6.3%); H. influenzae and S. pyogenes — in 1 sample (0.9%); S. pneumoniae and S. pyogenes — in 25 samples (22.3%). A triple combination consisting of S. pneumoniae, H. influenzae and S. pyogenes bacteria were detected together in 11 patients (9.8%). Meningococcal serogrouping revealed no connection with any of the 6 most common global serogroups responsible for epidemic incidence rise (A, B, C, W-135, X, Y). A clear tendency for prevalence of S. pyogenes DNA in adenoid pediatric biopsies in children diagnosed with “Adenoids and tonsils hypertrophy” vs. “Adenoids hypertrophy” was observed. It is noteworthy, a high relative prevalence of pneumococcal carriage (87.5%), found by us was of special importance. Pediatric carriers serving as a reservoir for virulent pneumococcal species pose a threat both for themselves and surrounding people. Thus, PCR-based data of adenoid biopsies may be a promising approach for future studies, as a potential to identify live viable but nonculturable bacteria in clinical specimens will contribute to a more accurate assessment of carriage rate of meningococci, pneumococci, H. influenzae and group A streptococci.

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Section: Discussionunclassified

Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

Комбарова

Бичучер

Soldatskiĭ

et al. 2020

Russian Journal of Infection and Immunity

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“…Examination of growth of bacterial species has little value in clinical practice due to the time needed for bacterial identification, combined with the fact that the identification of specific bacteria per se, such as Staphylococcus aureus , is not discriminatory for an infection. Noninfected wounds can show S. aureus growth; this bacterium can be present as a commensal . Moreover, studies also show that SSIs are caused by a wide range of bacterial species .…”

Section: Discussionmentioning

confidence: 99%

Variability in the diagnosis of surgical‐site infections after full‐thickness skin grafting: an international survey

et al. 2019

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Summary Background Diagnosis of a surgical‐site infection (SSI) in dermatological surgery can be based entirely on a subjective assessment, according to the fourth criterion of the most common definition of an SSI, which was established by the US Centers for Disease Control and Prevention. Objectives To investigate the interobserver agreement between dermatologists in their diagnosis of SSI of dermatosurgical wounds. Methods An international electronic photographic survey with eight photographs of wounds 1 week after full‐thickness skin grafting (FTSG) was sent to dermatologists. All wounds were assessed in terms of visual criteria beforehand. Data collected from respondents included physician characteristics and experience, and SSI assessments of all wounds. Results In total, 393 dermatologists from 27 countries enrolled. Most respondents were from the U.S.A. (25%), followed by Sweden (24%) and the U.K. (13%). There was only a slight interobserver agreement on SSI suspicion (κ = 0·19). SSI suspicion was lower for male physicians (P = 0·03), board‐certified dermatologists (P = 0·001), physicians regularly assessing surgical wounds (P = 0·03) and physicians performing FTSG (P < 0·001). Swedish physicians diagnosed more SSIs than U.S. physicians (P = 0·002). Erythema was more common in cases with higher SSI suspicion. Conclusions This study reveals broad inter‐rater variability in the diagnosis of SSI, illustrating the need for novel objective diagnostic methods that can better capture the variables that constitute an SSI.

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“…[22] Staphylococcus aureus strain USA300 MRSA (MR) and Staphylococcus aureus strain Newman MSSA (SA) are both invasive bacteria that colonize nasopharyngeal epithelium. [23,24] A detailed description of how C57BL/6J WT mice were infected, and how the pre-sepsis and sepsis phenotype was determined is based on prior work in the lab [25,26] and found in File S1, Supporting Information. Two biological replicates were used to collect MS-proteomics -three data collection technical replicates per biological sample.…”

Section: Mouse Models Of Sepsismentioning

confidence: 99%

Plasma Proteome Signature of Sepsis: a Functionally Connected Protein Network

et al. 2019

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Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ‐ and TMT‐based quantitative proteomics is reported here to study the plasma proteome in five mouse models of sepsis. A knowledge‐based interpretation of the data reveals a protein network with extensive connectivity through documented functional or physical interactions. The individual proteins in the network all have a documented role in sepsis and are known to be extracellular. The changes in protein abundance observed in the mouse models of sepsis have for the most part the same directionality (increased or decreased abundance) as reported in the literature for human sepsis. This network has been named the Plasma Proteome Signature of Sepsis (PPSS). The PPSS is a quantifiable molecular readout that can supplant the current symptom‐based approach used to diagnose sepsis. This type of molecular interpretation of sepsis, its progression, and its response to therapeutic intervention are an important step in advancing our understanding of sepsis, and for discovering and evaluating new therapeutic strategies.

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The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota

Cited by 265 publications

References 158 publications

Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

Variability in the diagnosis of surgical‐site infections after full‐thickness skin grafting: an international survey

Plasma Proteome Signature of Sepsis: a Functionally Connected Protein Network

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