The Commelina yellow mottle virus promoter drives companion-cell-specific gene expression in multiple organs of transgenic tobacco

Matsuda, Yoshie; Liang, Guanxiang; Zhu, Yali; Ma, Fengyun; Nelson, Richard S.; Ding, Biao

doi:10.1007/s00709-002-0027-6

Cited by 36 publications

(37 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CoYMVp is known to confer strong companion cellspecific expression (Medberry et al, 1992;Matsuda et al, 2002). A construct in which CoYMVp was fused to AtSUC2 complementary DNA (cDNA) was shown to rescue the Atsuc2-4 -/-knockout mutation (Srivastava et al, 2009b).…”

Section: Resultsmentioning

confidence: 99%

“…It was predicted that although these promoters had similar spatial expression as the natural AtSUC2p, differences in responses to stimuli could be used to maintain high levels of SUT expression and, consequently, high levels of phloem transport in conditions where transport may normally be down-regulated (Srivastava et al, 2009b). CoYMVp is from Commelina yellow mottle virus, but despite its viral origin, it is specific to phloem companion cells (Medberry et al, 1992;Matsuda et al, 2002). Research presented here demonstrates that while AtSUC2p is repressed, CoYMVp is up-regulated in the presence of elevated Suc levels and thus can be used to bypass possible endogenous regulation of AtSUC2 expression at the transcriptional level.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

et al. 2014

View full text Add to dashboard Cite

Sucrose (Suc) is the predominant form of carbon transported through the phloem from source to sink organs and is also a prominent sugar for short-distance transport. In all streptophytes analyzed, Suc transporter genes (SUTs or SUCs) form small families, with different subgroups evolving distinct functions. To gain insight into their capacity for moving Suc in planta, representative members of each clade were first expressed specifically in companion cells of Arabidopsis (Arabidopsis thaliana) and tested for their ability to rescue the phloem-loading defect caused by the Suc transporter mutation, Atsuc2-4. Sequence similarity was a poor indicator of ability: Several genes with high homology to AtSUC2, some of which have phloem-loading functions in other eudicot species, did not rescue the Atsuc2-4 mutation, whereas a more distantly related gene, ZmSUT1 from the monocot Zea mays, did restore phloem loading. Transporter complementary DNAs were also expressed in the companion cells of wild-type Arabidopsis, with the aim of increasing productivity by enhancing Suc transport to growing sink organs and reducing Suc-mediated feedback inhibition on photosynthesis. Although enhanced Suc loading and long-distance transport was achieved, growth was diminished. This growth inhibition was accompanied by increased expression of phosphate (P) starvation-induced genes and was reversed by providing a higher supply of external P. These experiments suggest that efforts to increase productivity by enhancing sugar transport may disrupt the carbon-to-P homeostasis. A model for how the plant perceives and responds to changes in the carbon-to-P balance is presented.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In this study, we generated transgenic tobacco expressing the 3a MP of Cucumber mosaic virus (CMV) fused to green fluorescent protein (GFP) under the control of the CC-specific promoter of Commelina yellow mottle virus (CoYMV) (Matsuda et al, 2002). We found that, in the absence of viral replication, the fusion protein trafficked from CC to SE and further from the "symplasmically isolated" SE-CC complex into neighboring cells.…”

Section: Introductionmentioning

confidence: 99%

Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

Itaya¹,

Ma²,

Qi³

et al. 2002

Plant Cell

Self Cite

View full text Add to dashboard Cite

Intercellular communication is essential for differentiation and development. In plants, plasmodesmata (PD) form cytoplasmic channels for direct communication. During plant development, programmed reduction in PD number and transport capacity creates the so-called symplasmic domains. Small fluorescent dyes and ions can diffuse among cells within a domain but not across domain boundaries. Such symplasmic isolation is thought to allow groups of cells to differentiate and develop into tissues with distinct structures and functions. Whether or how "symplasmically isolated" cells communicate with one another is poorly understood. One well-documented symplasmic domain is the sieve element-companion cell (SE-CC) complex in the phloem tissue. We report here that, when produced in the CC of transgenic tobacco, the 3a movement protein (3a MP) of Cucumber mosaic virus fused to green fluorescent protein (GFP) can traffic out of the SE-CC complex via PD. The extent of 3a MP:GFP traffic across the boundary between vascular and nonvascular tissues depends on organ type and developmental stage. Our findings provide experimental evidence that endogenous machinery exists for protein traffic between the symplasmically isolated SE-CC complex and neighboring cells. We suggest that PD-mediated traffic of selected macromolecules can be a mechanism for symplasmically isolated cells to communicate with one another.

show abstract

“…4A). The pCoYMV promoter is from Commelina Yellow Mottle Virus and known to confer strong companion cell-specific expression (Medberry et al, 1992;Matsuda et al, 2002). It is also up-regulated by Suc, whereas another companion cell-specific promoter, Arabidopsis SUCROSE-PROTON SYMPORTER2 (pAtSUC2), is repressed by Suc (Dasgupta et al, 2014).…”

mentioning

confidence: 99%

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Pizzio

Páez-Valencia²,

Khadilkar³

et al. 2015

Plant Physiol.

105

View full text Add to dashboard Cite

Phloem loading is a critical process in plant physiology. The potential of regulating the translocation of photoassimilates from source to sink tissues represents an opportunity to increase crop yield. Pyrophosphate homeostasis is crucial for normal phloem function in apoplasmic loaders. The involvement of Arabidopsis (Arabidopsis thaliana) type I proton-pumping pyrophosphatase (AVP1) in phloem loading was analyzed at genetic, histochemical, and physiological levels. A transcriptional AVP1 promoter:: GUS fusion revealed phloem activity in source leaves. Ubiquitous AVP1 overexpression (35S::AVP1 cassette) enhanced shoot biomass, photoassimilate production and transport, rhizosphere acidification, and expression of sugar-induced root ion transporter genes (POTASSIUM TRANSPORTER2 By contrast, phloem-specific AVP1 knockdown (pCoYMV:: RNAi AVP1) resulted in stunted seedlings in sucrose-deprived medium. We also present a promoter mutant avp1-2 (SALK046492) with a 70% reduction of expression that did not show severe growth impairment. Interestingly, AVP1 protein in this mutant is prominent in the phloem. Moreover, expression of an Escherichia colisoluble pyrophosphatase in the phloem (pCoYMV::pyrophosphatase) of avp1-2 plants resulted in severe dwarf phenotype and abnormal leaf morphology. We conclude that the Proton-Pumping Pyrophosphatase AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function.[

show abstract

The Commelina yellow mottle virus promoter drives companion-cell-specific gene expression in multiple organs of transgenic tobacco

Cited by 36 publications

References 19 publications

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

Arabidopsis Type I Proton-Pumping Pyrophosphatase Expresses Strongly in Phloem, Where It Is Required for Pyrophosphate Metabolism and Photosynthate Partitioning

Contact Info

Product

Resources

About