The Commelina Yellow Mottle Virus Promoter Is a Strong Promoter in Vascular and Reproductive Tissues

Medberry, Scott L.; Lockhart, Benham E; Olszewski, Neil E.

doi:10.2307/3869571

Cited by 23 publications

(29 citation statements)

References 11 publications

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“…This motif is also found in the FMV promoter [46]. A less conserved motif has also been identified in the CoYMV badnavirus promoter [34]. The asl element, characterized by TGACG direct repeats, binds to the AS1 nuclear factor [16] as well as the cloned TGA1 transcription factor [25].…”

Section: Discussionmentioning

confidence: 93%

“…Several transcriptional promoters which are capable of causing high levels of gene expression in transgenic plants have been isolated from pararetrovirus genomes [8,34,37,46]. During virus infection, these promoters direct the synthesis of genome-length RNAs which serve as templates for the reverse-transcriptase and as messenger RNAs for synthesis of viral proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Promoters isolated from badnaviruses, unlike caulimovirus promoters, seem to be primilary active in vascular tissues. Indeed, it has been reported that the rice tungro bacilliform virus (RTBV) and Commelina yellow mottle virus (CoYMV) promoters direct phloem-specific gene expression in transgenic plants [8,34,51].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

et al. 1996

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The cassava vein mosaic virus (CVMV) is a double stranded DNA virus which infects cassava plants (Manihot esculenta Crantz) and has been characterized as a plant pararetrovirus belonging to the caulimovirus subgroup. Two DNA fragments, CVP1 of 388 nucleotides from position -368 to +20 and CVP2 of 511 nucleotides from position -443 to +72, were isolated from the viral genome and fused to the uidA reporter gene to test promoter expression. The transcription start site of the viral promoter was determined using RNA isolated from transgenic plants containing the CVMV promoter:uidA fusion gene. Both promoter fragments were able to cause high levels of gene expression in protoplasts isolated from cassava and tobacco cell suspensions. The expression pattern of the CVMV promoters was analyzed in transgenic tobacco and rice plants, and revealed that the GUS staining pattern was similar for each construct and in both plants. The two promoter fragments were active in all plant organs tested and in a variety of cell types, suggesting a near constitutive pattern of expression. In both tobacco and rice plants, GUS activity was highest in vascular elements, in leaf mesophyll cells, and in root tips.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

et al. 1996

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“…This result indicates that there may be similar transcription factors in tobacco and rice that regulate expression of the promoter. It is known that some plant promoters retain specific expression patterns in different plant species (21)(22)(23)(24)(26)(27)(28)(29)(30)(31) while others do not (32,33).…”

Section: Discussionmentioning

confidence: 99%

Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants

Petruccelli

Dai

Carcamo

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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The promoter from rice tungro bacilliform badnavirus (RTBV) is expressed only in phloem tissues in transgenic rice plants. RF2a, a b-Zip protein from rice, is known to bind to the Box II cis element near the TATA box of the promoter. Here, we report that the full-length RTBV promoter and a truncated fragment E of the promoter, comprising nucleotides ؊164 to ؉45, result in phloemspecific expression of ␤-glucuronidase (GUS) reporter genes in transgenic tobacco plants. When a fusion gene comprising the cauliflower mosaic virus 35S promoter and RF2a cDNA was coexpressed with the GUS reporter genes, GUS activity was increased by 2-20-fold. The increase in GUS activity was positively correlated with the amount of RF2a, and the expression pattern of the RTBV promoter was altered from phloem-specific to constitutive. Constitutive expression of RF2a did not induce morphological changes in the transgenic plants. In contrast, constitutive overexpression of the b-ZIP domain of RF2a had a strong effect on the development of transgenic plants. These studies suggest that expression of the b-Zip domain can interfere with the function of homologues of RF2a that regulate development of tobacco plants. R egulation of transcription is achieved by the activity of multiple proteins that bind to regulatory elements, many of which are upstream of the promoters and alter basal rates of transcription initiation and͞or elongation (1, 2). To understand the mechanisms of tissue-specific and constitutive gene expression in plants, a number of promoters and transcription factors have been studied in recent years (3-16). It was shown that constitutive promoters, such as the cauliflower mosaic virus 35S promoter (17) and the promoter from cassava vein mosaic virus (14) are modular in organization and multiple cis elements. These elements, with specific transcription factors, apparently interact in an additive and͞or synergistic manner to confer gene expression in all plant tissues. Similarly, tissue-specific promoters contain multiple elements that contribute to promoter activity in both positive and negative ways (4-6, 8, 12, 18).The rice tungro bacilliform badnavirus (RTBV) promoter (19,20) and the transcription factors that interact it may serve as a model system to study plant tissue-specific gene expression. The virus, which replicates solely in phloem tissues, has a single promoter that is active in transfected protoplasts and is phloemspecific in transgenic rice plants (7,10,21,22).Within the fragment E of the promoter (nucleotides Ϫ164 to ϩ45), multiple cis elements were identified as being required for phloem-specific gene expression (7,10,15,23). A b-ZIP type transcription factor, RF2a, was isolated from rice and bound to Box II, a crucial cis element of the promoter. RF2a activates transcription from the RTBV promoter in an in vitro transcription system derived from rice cell cultures (11). Moreover, studies in transgenic rice plants suggested that RF2a is involved in the development of vascular tissues (11).We report here the functi...

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“…The GUS expression pattern under the regulation of the CFDV promoter is very similar to the activity described for CoYMV promoter/GUS constructs in transgenic tobacco plants [8]. Like CFDV, CoYMV is a monocotyledon-infecting plant virus, but belonging to the badnavirus group with a double-stranded circular DNA genome of 7489 nucleotides [7] as compared to the singlestranded circular CFDV DNA of 1291 nucleotides [ 12].…”

Section: Jr6mentioning

confidence: 71%

The promoter of coconut foliar decay-associated circular single-stranded DNA directs phloem-specific reporter gene expression in transgenic tobacco

Rohde¹,

Becker²,

Randles

1995

Plant Mol Biol

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A full-length double-stranded DNA copy of the single-stranded circular DNA associated with coconut foliar decay virus (CFDV) was constructed. Full-length CFDV DNA and smaller fragments were transcriptionally fused to the beta-glucuronidase reporter gene and examined for promoter activity in vivo. In stably transformed tobacco plants, the CFDV DNA promoter confered a tissue-specific expression pattern in that the reporter gene was specifically expressed in the phloem tissue of the vascular system in stem, leaves and flower. These results are in agreement with the previously reported association of CFDV DNA with the phloem of its coconut host plant.

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The Commelina Yellow Mottle Virus Promoter Is a Strong Promoter in Vascular and Reproductive Tissues

Cited by 23 publications

References 11 publications

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter

Transcription factor RF2a alters expression of the rice tungro bacilliform virus promoter in transgenic tobacco plants

The promoter of coconut foliar decay-associated circular single-stranded DNA directs phloem-specific reporter gene expression in transgenic tobacco

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