The Commelina yellow mottle virus promoter is a strong promoter in vascular and reproductive tissues.

Abstract

Commelina yellow mottle virus (CoYMV) is a double-stranded DNA virus that infects the monocot Commelina diffusa. Although CoYMV and cauliflower mosaic virus (CaMV; another double-stranded DNA virus) probably replicate by a similar mechanism, the particle morphology and host range of CoYMV place it in a distinct group. We present evidence that a promoter fragment isolated from CoYMV confers a tissue-specific pattern of expression that is different from that conferred by the CaMV 35s promoter. When the CoYMV promoter is used to drive expression of the P-glucuronidase reporter gene in stably transformed tobacco plants, 0-glucuronidase activity occurs primarily in the phloem, the phloemassociated cells, and the axial parenchyma of roots, stems, leaves, and flowers. Activity is also detected throughout the anther, with highest activity in the tapetum. In contrast, the CaMV 35s promoter is active in most cell types. The CoYMV promoter is a strong promoter, and when the activity of the CoYMV promoter is compared with that of a duplicated CaMV 35s promoter, it is 30% as active in tobacco suspension cells and up to 25% as active in maize suspension cells. These properties of the CoYMV promoter make it potentially useful for high-leve1 expression of engineered genes in vascular cells.

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

“…CoYMVp is known to confer strong companion cellspecific expression (Medberry et al, 1992;Matsuda et al, 2002). A construct in which CoYMVp was fused to AtSUC2 complementary DNA (cDNA) was shown to rescue the Atsuc2-4 -/-knockout mutation (Srivastava et al, 2009b).…”

“…It was predicted that although these promoters had similar spatial expression as the natural AtSUC2p, differences in responses to stimuli could be used to maintain high levels of SUT expression and, consequently, high levels of phloem transport in conditions where transport may normally be down-regulated (Srivastava et al, 2009b). CoYMVp is from Commelina yellow mottle virus, but despite its viral origin, it is specific to phloem companion cells (Medberry et al, 1992;Matsuda et al, 2002). Research presented here demonstrates that while AtSUC2p is repressed, CoYMVp is up-regulated in the presence of elevated Suc levels and thus can be used to bypass possible endogenous regulation of AtSUC2 expression at the transcriptional level.…”

Cis-regulatory elements used to control gene expression in plants

“…This tandem promoter region of zein gene provides constitutive expression also in potato (Medberry et al 1992;Torbert et al 1998).…”

Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced

“…1A), in which 35S is the 35S RNA promoter from cauliflower mosaic virus, cre is the coding region for Cre recombinase, and lox is the 34-bp recombination site incorporated into the leader sequence of the transcript. This was achieved by transforming leaf mesophyll protoplasts, derived from 35S-lox-cre plants, with the pEL1 plasmid that contains lox-hpt and Cp-gus, in which hpt is the hygromycin phosphotransferase coding region, gus is the ␤-glucuronidase coding region, and Cp is the commelina yellow mottle virus promoter (Medberry et al 1992). Cre-mediated integration of pEL1 into the genomic 35S-lox-cre target confers a hygromycin resistant (Hyg R ) phenotype through a 35S-lox-hpt linkage, and abolishes cre transcription through displacement of the 35S promoter.…”