The Comet Assay: A Reliable and Sensitive Method for the Documentation of UV-B Induced DNA Damage

Breipohl, W.; Penzkofer, C.; Naib-Majani, W.; Rauwolf, M.; Leip, O.; Augustin, Albert J.; Leyendecker, M.

doi:10.1007/978-1-4615-1887-7_40

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“…The relevant pathways are still poorly understood. However, according to ongoing investigations on lens ep ithelial and corneal endothelial cells by others and ourselves, it can be assumed that control factors in cell proliferation and lens homeosta sis also involve regionality of oxygen free rad icals and respective scavenging systems [6,7,14,15].…”

Section: Discussionmentioning

confidence: 99%

“…One might postulate conformational DNA changes with extended culture periods underlying these and other phe nomena such as lack of tumorigenic capacity of lens epithelial cells [13]. In fact, DNA comet assay investigations on lens epithelial cells in culture suggest differences in DNA susceptibil ity to culture conditions and UV irradiation, as a function of cell culture passages [14,15]. UV susceptibility of cultured lens epithelial cells might further be caused by impaired metabo lism and energy homeostasis of the cells [10].…”

Section: Discussionmentioning

confidence: 99%

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Effects of UV-B on the Growth Pattern of Bovine Passage I and II Lens Epithelial Cells in vitro

Breipohl

Leyendecker²,

Tiesenhausen³

et al. 1995

Ophthalmic Res

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To further evaluate the pivotal role of epithelial cell proliferation in lens homeostasis, this study compares the principles of regional growth control in central and preequatorial bovine lens epithelial cells in culture. Central lens epithelial cells do not proliferate in vivo while preequatorial cells do. In tissue culture, both central and preequatorial cells proliferate, albeit at different rates. At all stages investigated, nonirradiated passage I central cells proliferated less extensively when compared to the preequatorial cells. In contrast, central passage II cells showed a higher proliferation rate than peripheral cells until day 7, when the situation reversed. UV-B radiation led to a dose-dependent reduction of cell proliferation but did not change the principle cytokinetic differences between central and peripheral cells in passage I cells. Under all circumstances, passage I cells grew more intensively than their passage II counterparts. Data on origin-related differences in cell proliferation of cultured lens epithelial cells suggests growth control features other than just the regionally limited expression of growth factor receptors in the preequatorial extracellular matrix and cell membranes. Investigations are seen as an important step towards a better understanding of the features underlying regional proliferation control and its impairments, at least in lens epithelial cells.

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Section: Discussionmentioning

confidence: 99%