The combined use of ozone therapy and autologous platelet-rich plasma as an alternative approach to foot rot treatment for sheep. A preliminary study

Szponder, Tomasz; Wessely-Szponder, Joanna; Świeca, Michał; Smolira, A.; Gruszecki, Tomasz

doi:10.1016/j.smallrumres.2017.08.015

Cited by 14 publications

(24 citation statements)

References 25 publications

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“…It should be also outlined that the AOB approach has been recently applied as a biomarker of blood antioxidant status in ewes fed with Ascophyllum nodosum and flaxseed under high ambient temperature [81] and in sheep suffering foot rot and treated with ozone therapy [82], thus demonstrating its high versatility and applicability both in human and animal studies.…”

Section: Evaluation Of Human Blood Antioxidant Status After Food Imentioning

confidence: 99%

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

et al. 2018

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In the last decades, increasing demand of antioxidant-rich foods and growing interest in their putative role in prevention of degenerative diseases have promoted development of methods for measuring Antioxidant Capacity (AC). Nevertheless, most of these assays use radicals and experimental conditions far from the physiological ones, and are able to estimate only one or a few antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX–FL methods, based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a more comprehensive AC evaluation. In fact, they are able to detect simultaneously many antioxidant functions (scavenging of some physiological radical species, iron ion reducing and chelating activities, inhibition of the pro-oxidant apoenzyme) and to highlight synergism among phytochemicals. They are applied to dissect antioxidant properties of several natural plant products: food-grade antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. Recently, LOX–FL has been used for ex vivo AC measurements of human blood samples after short- and long-term intakes of some of these foods, and the effectiveness in improving serum antioxidant status was evaluated using the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level ratio. An overview of data is presented.

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Section: Evaluation Of Human Blood Antioxidant Status After Food Imentioning

confidence: 99%

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

et al. 2018

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“…Then the plasma fraction was collected and centrifuged (400 × g , 15 min). The top layer was discarded and the remaining volume was platelet-rich plasma (23).…”

Section: Methodsmentioning

confidence: 99%

“…The main rationale for the use of PRP stems from the growth factors released from platelet α-granules, including platelet-derived growth factor (PDGF), transforming growth factor (TGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor (IGF) (20). All these growth factors were detected previously in preparations of PRP from ovine blood together with antimicrobial peptide (PF4), which enhances their antimicrobial properties (23). These antimicrobial properties of platelet components would be best taken advantage of producing a combined neutrophil and platelet extract to maximise their activity (2).…”

Section: Introductionmentioning

confidence: 90%

Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

Wessely-Szponder

Szponder

Bobowiec

et al. 2019

Journal of Veterinary Research

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Introduction: Antimicrobial peptides (AMP) are a large group of innate immune effectors, which apart from antimicrobial activity show immunomodulative properties. Platelet-rich plasma (PRP) is a source of autologous growth factors and is used for stimulation of bone and soft tissue healing. The purpose of this study was to assess the influence of PRP and AMP extract on ovine monocyte-derived macrophage cultures. Material and Methods: The study was conducted on ovine macrophages (Mfs) previously stimulated with LPS or dexamethasone and then with preparations of PRP or AMP. Following activation of the Mfs their morphological and functional features were assessed. Results: The study revealed pro-inflammatory influence of both examined preparations on Mfs cultures on the basis of morphology, ROS generation and arginase activity. Both preparations enhanced the pro-inflammatory response of cultured Mfs. Conclusion: This activity may intensify the antimicrobial action of Mfs, however, in cases of excessive and prolonged inflammation the use of these preparations should be limited.

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“…A study performed by Szponder et al. (2017) selected 15 sheep, which 10 were suspected of foot rot and 5 were healthy.…”

Section: Presentation Of the Original Articles Analyzedmentioning

confidence: 99%

Ozone and its derivatives in veterinary medicine: A careful appraisal

Orlandin

Machado

Ambrósio

et al. 2021

Veterinary and Animal Science

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Highlights In the Vet field, there are lot of scientific publications with missing or incomplete data, poor case reports and papers without a control group or unrepresentative sample. Differences between animal and human blood composition, anatomy and physiology must be taken into consideration, especially in therapies not yet fully approved. Some practitioners produce their own ozonated solution or autohemotherapy, in the absence of information regarding the compatibility of the material used. Other than the properties of the commercial preparations, the standardization of both treatment methods and times influence the results obtained with ozone therapy.

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The combined use of ozone therapy and autologous platelet-rich plasma as an alternative approach to foot rot treatment for sheep. A preliminary study

Cited by 14 publications

References 25 publications

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview

Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

Ozone and its derivatives in veterinary medicine: A careful appraisal

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