The combined strategy of mesenchymal stem cells and tissue-engineered scaffolds for spinal cord injury regeneration

Libro, Rosaliana; Bramantı, Placido; Mazzon, Emanuela

doi:10.3892/etm.2017.4939

Cited by 35 publications

(22 citation statements)

References 114 publications

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“…Furthermore, specific organization of ECM proteins within the DMS provides guided hospitable microenvironment for more synchronized cellular engraftment and function. Several other studies have attempted to use such bioengineering approaches using different kinds of biological and synthetic scaffolds, however reported to provide insufficiency of desired mechanical strength, reduced growth factors signaling and least regenerative support (Nomura et al, 2006 ; Zahir et al, 2008 ; Wang et al, 2012 ; Shrestha et al, 2014 ; Libro et al, 2017 ; Liu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury

Vishwakarma

Bardia

Lakkireddy

et al. 2018

Front. Bioeng. Biotechnol.

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Spinal cord injury (SCI) is one of the most devastating conditions echoes with inflammation, enhanced fibrosis and larger axonal gaps due to destruction of neurological cells which has caused continuous increasing mortality rate of SCI patients due to absence of suitable treatment modalities. The restoration of structural and functional aspect of damaged neurological tissues at the lesion site in spinal cord has been challenging. Recent developments have showed tremendous potential of neural stem cell-based strategies to form a neuronal relay circuit across the injury gap which facilitates some levels of improvement in SCI condition. However, to provide better therapeutic responses, critical mass of grafted cells must survive for long-term and differentiate into neuronal cells with well-developed axonal networks. Hence, development of tissue specific biological neuronal constructs is highly desirable to provide mechanical and biological support for long-term survival and function of neurological cells within natural biological niche. In this study, we report development of a tissue specific neuronal constructs by culturing human neural precursor cells on decellularized meningeal scaffolds to provide suitable biological neuronal construct which can be used to support mechanical, structural and functional aspect of damaged spinal cord tissues. This particular tissue specific biological construct is immunologically tolerable and provides precisely orchestral three-dimensional platform to choreograph the long-distance axonal guidance and more organized neuronal cell growth. It passes sufficient mechanical and biological properties enriched with several crucial neurotrophins required for long-term survival and function of neurological cells which is required to form proper axonal bridge to regenerate the damaged axonal connectomes at lesion-site in SCI.

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Section: Discussionmentioning

confidence: 99%

Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury

Vishwakarma

Bardia

Lakkireddy

et al. 2018

Front. Bioeng. Biotechnol.

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“…The injury may result in tissue loss and neurologic dysfunction, such as paralysis or diminished muscle strength, movement, or sensation below the injured region (W. R. Kim et al, 2017). SCI has a high mortality and disability rate, which affects approximately 250,000-500,000 people each year, and it has become a huge financial burden for health care (Libro, Bramanti, & Mazzon, 2017). However, there is still no effective therapy in clinic (Hurlbert & Hamilton, 2008).…”

mentioning

confidence: 99%

Signatures of altered long noncoding RNAs and messenger RNAs expression in the early acute phase of spinal cord injury

Shi

Ning

Zhang

et al. 2018

Journal Cellular Physiology

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Spinal cord injury (SCI) is a highly severe disease and it can lead to the destruction of the motor and sensory function resulting in temporary or permanent disability. Long noncoding RNAs (lncRNAs) are transcripts longer than 200 nt that play a critical role in central nervous system (CNS) injury. However, the exact roles of lncRNAs and messenger RNAs (mRNAs) in the early acute phase of SCI remain to be elucidated. We examined the expression of mRNAs and lncRNAs in a rat model at 2 days after SCI and identified the differentially expressed lncRNAs (DE lncRNAs) and differentially expressed mRNAs (DE mRNAs) using microarray analysis. Subsequently, a comprehensive bioinformatics analysis was also performed to clarify the interaction between DE mRNAs. A total of 3,193 DE lncRNAs and 4,308 DE mRNAs were identified between the injured group and control group. Classification, length distribution, and chromosomal distribution of the dysregulated lncRNAs were also performed. The gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed to identify the critical biological processes and pathways. A protein−protein interaction (PPI) network indicated that IL6, TOP2A, CDK1, POLE, CCNB1, TNF, CCNA2, CDC20, ITGAM, and MYC were the top 10 core genes. The subnetworks from the PPI network were identified to further elucidate the most significant functional modules of the DE mRNAs. These data may provide novel insights into the molecular mechanism of the early acute phase of SCI. The identification of lncRNAs and mRNAs may offer potential diagnostic and therapeutic targets for SCI.

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“…In fact, scaffolds promote MSC adhesion and their survival. The effects of cell-seeded biomaterial scaffolds on improved axonal regrowth or enhanced functional outcomes after SCI than scaffolds alone have been shown in many studies ( Hejcl et al, 2010 ; Kim et al, 2016 ; Blasko et al, 2017 ; Peng et al, 2018 ; Zaviskova et al, 2018 ) and reviewed in Libro et al (2017) and Yousefifard et al (2019) .…”

Section: Biomaterials In Combination With Mscs In Sci Treatmentmentioning

confidence: 99%

Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis

Syková

Cizkova

Kubinová

2021

Front. Cell Dev. Biol.

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Preclinical and clinical studies with various stem cells, their secretomes, and extracellular vesicles (EVs) indicate their use as a promising strategy for the treatment of various diseases and tissue defects, including neurodegenerative diseases such as spinal cord injury (SCI) and amyotrophic lateral sclerosis (ALS). Autologous and allogenic mesenchymal stem cells (MSCs) are so far the best candidates for use in regenerative medicine. Here we review the effects of the implantation of MSCs (progenitors of mesodermal origin) in animal models of SCI and ALS and in clinical studies. MSCs possess multilineage differentiation potential and are easily expandable in vitro. These cells, obtained from bone marrow (BM), adipose tissue, Wharton jelly, or even other tissues, have immunomodulatory and paracrine potential, releasing a number of cytokines and factors which inhibit the proliferation of T cells, B cells, and natural killer cells and modify dendritic cell activity. They are hypoimmunogenic, migrate toward lesion sites, induce better regeneration, preserve perineuronal nets, and stimulate neural plasticity. There is a wide use of MSC systemic application or MSCs seeded on scaffolds and tissue bridges made from various synthetic and natural biomaterials, including human decellularized extracellular matrix (ECM) or nanofibers. The positive effects of MSC implantation have been recorded in animals with SCI lesions and ALS. Moreover, promising effects of autologous as well as allogenic MSCs for the treatment of SCI and ALS were demonstrated in recent clinical studies.

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The combined strategy of mesenchymal stem cells and tissue-engineered scaffolds for spinal cord injury regeneration

Cited by 35 publications

References 114 publications

Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury

Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury

Signatures of altered long noncoding RNAs and messenger RNAs expression in the early acute phase of spinal cord injury

Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis

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