The Combined Strategy for iron uptake is not exclusive to domesticated rice (Oryza sativa)

Wairich, Andriele; Oliveira, Ben Hur Neves de; Arend, Ezequiel Barth; Duarte, Guilherme Coutinho Kullmann; Ponte, Lucas Roani; Sperotto, Raul Antônio; Ricachenevsky, Felipe Klein; Fett, Janette Palma

doi:10.1038/s41598-019-52502-0

Cited by 76 publications

(44 citation statements)

References 76 publications

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“…In addition, this gene was not able to complement the fet3fet4 yeast mutant, which is defective in the Fe uptake when provided with Fe 2+ -nicotianamine (Bashir, et al 2015;Vasconcelos MW Characterization of the PT Clade of Oligopeptide Transporters in Rice), although it seems to play a significant role in Fe distribution within rice plants (Bashir, et al 2015). MIR was up regulated in opt7 lines both in Fe sufficient and Fe deficient medium when compared with wild type plants cultivated under the same condition (Bashir, et al 2015) corroborating the results obtained by the co-expression network and highlighting the (Wairich, et al 2019). These genes encode proteins ranging from 56 to 59 amino acids long with no predicted conserved domains.…”

Section: Mir Co-expression Analyses and Its Relevance To Fe Deficiencsupporting

confidence: 63%

“…MIR was previously characterized as functionally important for Fe homeostasis in cultivated rice, and independent expression data has corroborated its role in Fe deficiency responses (Bashir, et al 2014;Ishimaru, et al 2009;Stein, et al 2019;Wairich, et al 2019) The MIR loss-of-function mutant (mir) is impaired in correctly perceiving Fe sufficiency status, which leads to increased Fe uptake, up regulation of Fe deficiency response genes, and Fe accumulation (Ishimaru, et al 2009). MIR protein was localized to mitochondria, and mir plants show somewhat similar phenotypic changes to the mitochondrial iron transporter (MIT) mit loss-of-function plants (Bashir, et al 2011).…”

Section: Mir Function May Still Be Evolving In Distinct Oryza Lineagesmentioning

confidence: 98%

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The Mitochondrial Iron Regulated (MIR) gene is Oryza genus-specific and evolved before the speciation of major AA-genome lineages

Wairich

et al. 2019

Preprint

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Rice (Oryza sativa L.) is both a model species and an economically relevant crop. The Oryza genus comprises 25 species, which constitute a genetic reservoir for cultivated rice breeding. Genomic data is available for several Oryza species, making it a good model for genetics and evolution within closely related species. The Mitochondrial Iron Regulated (MIR) gene was previously implicated in O. sativa Fe deficiency response, and was considered an orphan gene present only in rice. Here we show that MIR is also found in other Oryza species that belong to the AA genome group. We characterized the evolutionary pattern of MIR genes within the Oryza genus. Our data suggest that MIR originated de novo from non-coding sequences present only in AA genome species, but these sequences in turn are derived from an exon fragment of Raffinose Synthase genes, present in several groups of monocots. We also show that all species that have a putative functional MIR conserve their regulation by Fe deficiency, with the exception of Oryza barthii. In O. barthii, the MIR coding sequence was translocated to a different chromosomal position and separated from its regulatory region, which led to a lack of Fe deficiency responsiveness. Moreover, we show that MIR co-expression subnetwork cluster in O. sativa is responsive to Fe deficiency, evidencing the importance of the newly originated gene in Fe uptake. This work establishes that MIR is not an orphan gene as previously proposed, but a de novo originated gene within the Oryza genus. We also showed that MIR is undergoing genomic changes in at least one species (O. barthii), which can impact its role in Fe deficiency. P

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Section: Mir Co-expression Analyses and Its Relevance To Fe Deficiencsupporting

confidence: 63%

Section: Mir Function May Still Be Evolving In Distinct Oryza Lineagesmentioning

confidence: 98%

The Mitochondrial Iron Regulated (MIR) gene is Oryza genus-specific and evolved before the speciation of major AA-genome lineages

Wairich

et al. 2019

Preprint

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“…Ferric-mugineic acid family phy-tosiderophore chelates are subsequently transported into the root via YSL transporters and reduced for utilization after uptake. Moreover, the response of rice and other monocots to iron deficiency differs from that of maize by utilizing aspects of both Strategy I and II for iron uptake (49).…”

Section: Overview Of Plant Iron Metabolismmentioning

confidence: 99%

Iron homeostasis and plant immune responses: Recent insights and translational implications

Herlihy

Long

McDowell

2020

Journal of Biological Chemistry

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Iron (Fe) metabolism and the plant immune system are both critical for plant vigor in natural ecosystems and for reliable agricultural productivity. Mechanistic studies of plant iron homeostasis and plant immunity have traditionally been carried out in isolation from each other; however, our growing understanding of both processes has uncovered significant connections. For example, iron plays a critical role in the generation of reactive oxygen intermediates during immunity and has been recently implicated as a critical factor for immune-initiated cell death via ferroptosis. Moreover, plant iron stress triggers immune activation, suggesting that sensing of iron depletion is a mechanism by which plants recognize a pathogen threat. The iron deficiency response engages hormone signaling sectors that are also utilized for plant immune signaling, providing a probable explanation for iron-immunity cross-talk. Finally, interference with iron acquisition by pathogens might be a critical component of the immune response. Efforts to address the global burden of iron deficiency-related anemia, have focused on classical breeding and transgenic approaches to develop crops biofortified for iron content. However, our improved mechanistic understanding of plant iron metabolism suggests that such alterations could promote or impede plant immunity, depending on the nature of the alteration and the virulence strategy of the pathogen. Effects of iron biofortification on disease resistance should be evaluated while developing plants for iron biofortification.

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“…Despite the clear distinction usually made between graminaceous and non-graminaceous species for the type of strategy employed, the picture is not so clear-cut. Roots of Strategy I plants have been described to exude metabolites such as phenolics that may play a role in Fe uptake by acting as reducing and/or chelating agents 5 , whilst Fe-starved Strategy II species such as rice and maize have been observed to induce IRT-encoding genes 6 – 8 . For this reason, a combined strategy has been recently postulated for the uptake of this micronutrient by grass species 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Marastoni

Lucini

Miras-Moreno

et al. 2020

Sci Rep

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In several cultivation areas, grapevine can suffer from Fe chlorosis due to the calcareous and alkaline nature of soils. This plant species has been described to cope with Fe deficiency by activating Strategy I mechanisms, hence increasing root H+ extrusion and ferric-chelate reductase activity. The degree of tolerance exhibited by the rootstocks has been reported to depend on both reactions, but to date, little emphasis has been given to the role played by root exudate extrusion. We studied the behaviour of two hydroponically-grown, tolerant grapevine rootstocks (Ramsey and 140R) in response to Fe deficiency. Under these experimental conditions, the two varieties displayed differences in their ability to modulate morpho-physiological parameters, root acidification and ferric chelate reductase activity. The metabolic profiling of root exudates revealed common strategies for Fe acquisition, including ones targeted at reducing microbial competition for this micronutrient by limiting the exudation of amino acids and sugars and increasing instead that of Fe(III)-reducing compounds. Other modifications in exudate composition hint that the two rootstocks cope with Fe shortage via specific adjustments of their exudation patterns. Furthermore, the presence of 3-hydroxymugenic acid in these compounds suggests that the responses of grapevine to Fe availability are rather diverse and much more complex than those usually described for Strategy I plants.

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The Combined Strategy for iron uptake is not exclusive to domesticated rice (Oryza sativa)

Cited by 76 publications

References 76 publications

The Mitochondrial Iron Regulated (MIR) gene is Oryza genus-specific and evolved before the speciation of major AA-genome lineages

The Mitochondrial Iron Regulated (MIR) gene is Oryza genus-specific and evolved before the speciation of major AA-genome lineages

Iron homeostasis and plant immune responses: Recent insights and translational implications

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

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