The combined formulation of brassinolide and pyraclostrobin increases biomass and seed yield by improving photosynthetic capacity in Arabidopsis thaliana

An, Ya-Qi; Qin, Zi-Ting; Li, Dandan; Zhao, Ruiqi; Bi, Bo-Shi; Wang, Dawei; Ma, Dejun; Xi, Zhenfeng

doi:10.3389/fpls.2023.1138563

Cited by 4 publications

(16 citation statements)

References 76 publications

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“…Research has found that some fungicides could promote plant growth. 53 Therefore, to explore whether our newly synthesized compounds could promote plant growth or not, compound Ip with promising fungicidal activity was selected for the subsequent plant growth-promoting activity experiments. The results showed that Ip showed higher activity than pydiflumetofen in promoting the growth of wheat seedlings at concentrations of 2−20 μM (Table S4).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Discovery of Benzothiazolylpyrazole-4-Carboxamides as Potent Succinate Dehydrogenase Inhibitors through Active Fragment Exchange and Link Approach

Yin,

Sun,

Wang

et al. 2023

J. Agric. Food Chem.

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Succinate dehydrogenase (SDH) is an attractive target for developing green fungicides to manage agricultural pathogens in modern agriculture research. Herein, in this work, we report the discovery of benzothiazolylpyrazole-4-carboxamides I-III as potent SDH inhibitors using active fragment exchange and link approach. The results of the fungicidal activity assays showed that some of the synthesized compounds exhibited excellent inhibition against the tested fungi. Systematic structure−activity relationship studies led to the discovery of compound Ip, N- (1-((4,6-difluorobenzo[d]thiazol-2-yl)thio)propan-2-yl)-3-(difluoromethyl)-N-methoxy-1-methyl-1H-pyrazole-4-carboxamide, which showed higher fungicidal activity against Fusarium graminearum Schw (EC 50 = 0.93 μg/mL) than the commercial fungicides thifluzamide (EC 50 > 50 μg/mL) and boscalid (EC 50 > 50 μg/mL). The molecular simulation studies suggested that hydrophobic interactions were the primary driving forces between ligands and SDH. Promisingly, we found that Ip could stimulate the growth of wheat seedlings and Arabidopsis thaliana and increase the biomass of the treated plants. Preliminary studies on the plant growth promoter mechanism of Ip indicated that it could increase nitrate reductase activity in planta, that, in turn, stimulates the growth of plants.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…Research has found that some fungicides could promote plant growth . Therefore, to explore whether our newly synthesized compounds could promote plant growth or not, compound Ip with promising fungicidal activity was selected for the subsequent plant growth-promoting activity experiments.…”

Section: Resultsmentioning

confidence: 99%

Discovery of Benzothiazolylpyrazole-4-Carboxamides as Potent Succinate Dehydrogenase Inhibitors through Active Fragment Exchange and Link Approach

Yin,

Sun,

Wang

et al. 2023

J. Agric. Food Chem.

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Section: Methodsmentioning

confidence: 99%

“…The concentration range of BL and Pyr was chosen based on our previous study with A. thaliana, where exogenous application of Pyr exceeding 3 μM and BL exceeding 1 μM both caused phytotoxicity, while exogenous application of Pyr below 0.03 μM and BL below 0.1 μM both did not significantly alter plant phenotype. 29 For the subsequent experiments to comprehensively study the effect of BL + Pyr on disease resistance, the uniform seedlings were picked out and randomly divided into 4 groups. Those 4 groups were foliar sprayed with 1‰ DMSO, BL (1 μM), Pyr (3 μM), and a mixture of BL (1 μM) and Pyr (3 μM), respectively.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The construction of mRNA libraries and data analysis was conducted according to our previous study. 29 In this study, the differentially expressed genes (DEGs) were assigned as the genes with a FDR (the false discovery rate) < 0.05. The enrichment analysis of gene ontology (GO) terms and KEGG pathways was conducted on the GO (http://geneontology.org/) and KEGG databases (https://www.kegg.jp/kegg/pathway.html), respectively.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Co-application of Brassinolide and Pyraclostrobin Improved Disease Control Efficacy by Eliciting Plant Innate Defense Responses in Arabidopsis thaliana

An,

Bi,

et al. 2023

J. Agric. Food Chem.

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Applying brassinolide (BL, a phytohormone) in combination with pyraclostrobin (Pyr, a fungicide) has shown effective disease control in field trials. However, the mechanism by which BL + Pyr control disease remains uncertain. This work compared the disease control and defense responses of three pretreatments (BL, Pyr, and BL + Pyr) in Arabidopsis thaliana. We found that BL + Pyr improved control against Pyr-sensitive Hyaloperonospora arabidopsidis and Botrytis cinerea by 19 and 17% over Pyr, respectively, and achieved 29% control against Pyr-resistant B. cinerea. Furthermore, BL + Pyr outperformed BL or Pyr in boosting transient H 2 O 2 accumulation, and the activities of POD, APX, GST, and GPX. RNA-seq analysis revealed a more potent activation of defense genes elicited by BL + Pyr than by BL or Pyr. Overall, BL + Pyr controlled disease by integrating the elicitation of plant innate disease resistance with the fungicidal activity of Pyr.

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Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

Zhou,

Zhang,

et al. 2023

IJMS

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Interspecific crosses that fuse the genomes of two different species may result in overall gene expression changes in the hybrid progeny, called ‘transcriptome shock’. To better understand the expression pattern after genome merging during the early stages of allopolyploid formation, we performed RNA sequencing analysis on developing embryos of Brassica rapa, B. napus, and their synthesized allotriploid hybrids. Here, we show that the transcriptome shock occurs in the developing seeds of the hybrids. Of the homoeologous gene pairs, 17.1% exhibit expression bias, with an overall expression bias toward B. rapa. The expression level dominance also biases toward B. rapa, mainly induced by the expression change in homoeologous genes from B. napus. Functional enrichment analysis revealed significant differences in differentially expressed genes (DEGs) related to photosynthesis, hormone synthesis, and other pathways. Further study showed that significant changes in the expression levels of the key transcription factors (TFs) could regulate the overall interaction network in the developing embryo, which might be an essential cause of phenotype change. In conclusion, the present results have revealed the global changes in gene expression patterns in developing seeds of the hybrid between B. rapa and B. napus, and provided novel insights into the occurrence of transcriptome shock for harnessing heterosis.

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The combined formulation of brassinolide and pyraclostrobin increases biomass and seed yield by improving photosynthetic capacity in Arabidopsis thaliana

Cited by 4 publications

References 76 publications

Discovery of Benzothiazolylpyrazole-4-Carboxamides as Potent Succinate Dehydrogenase Inhibitors through Active Fragment Exchange and Link Approach

Discovery of Benzothiazolylpyrazole-4-Carboxamides as Potent Succinate Dehydrogenase Inhibitors through Active Fragment Exchange and Link Approach

Co-application of Brassinolide and Pyraclostrobin Improved Disease Control Efficacy by Eliciting Plant Innate Defense Responses in Arabidopsis thaliana

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

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