The Combined Effects of Nano-Zinc, Nano-Albumin and Honey in Healing Wounds Caused by Third-Degree Burn in Male Mice

Abstract

Background: There is extensive evidence on the healing properties of honey. Therefore, in this study the effect of nano zinc and nano-albumin combination in topical treatment of third-degree burn was evaluated. Methods: In this experimental study, 16 adult male mice were randomly divided to experimental group 1 (treated with honey and nano-zinc), experimental group 2 (treated with nano-albumin and honey), experimental group 3 (treated with honey, nano-albumin, and nano-zinc), and group 4 (no treatment). Under sterile conditions and using general anesthesia, third-degree burns on the back of each mouse was created. Topical burn treatment was performed once a day for 6 weeks, and after the end of treatment, the mice were scarified by anesthesia and then a sample of wound bed of mice was prepared and fixed in formalin. A histological process was performed on the samples. The specimens were stained with H and E staining and microscopic observations were made. Results: At the macroscopic level, after the treatment of burn wounds, shrinkage of burn wound and scar reduction was observed in honey and nano-zinc treated group compared to the control and other treated groups. Histological studies showed a significant increase in total thickness of dermal and epidermal layers of skin in this experimental group compared to other groups (P < 0.05). Discussion: The results showed that the combination of organic honey and nano-zinc could accelerate the healing of burn wounds in mice, while adding nano-albumin to the mixture had opposite effects and could delay wound healing.

Wound-Healing Activity of Green and Chemical Zinc Oxide Nanoparticles (ZnO-NPs) Gels in Equine Wounds: A clinical Study

“…Plants are the most preferred source of NPs synthesis because they lead to the production of stable, varied in shape and size NPs (Qu et al, 2011). We could explain our results in light of the previous reports which proved that, Zinc oxide nanoparticles accelerate collagen synthesis and wound shrinkage and reducing scars after healing wounds (Saremi et al, 2016). Zinc ions (Zn+2) which released from ZnO can enhance keratinocyte migration towards the wound site so promote the healing (Kumar et al, 2012).…”