The combined effect of fludarabine monophosphate and radiation as well as gemcitabine and radiation on squamous carcinoma tumor cell lines<i>in vitro</i>

Abstract-Recently the number of cancer case has been increasing. In addition, because of aging of the population and diversification of values among the people, less-invasive and high-QOL (quality of life) treatment for tumors has been needed. BNCT (Boron Neutron Capture Therapy) is based on the nuclear reaction of two essentially nontoxic species, 10 B and thermal neutron. BNCT is effective and minimally invasive treatment. However BNCT is inefficient because atomic reactor is used for neutron source. In this study, we proposed to BNCT using an accelerator and new boron agents; HVJ (Hemagglutinating Virus of Japan) envelope (HVJ-E) and Boronated liposome (B-Liposome). HVJ-E is a nonviral vector using inactivated virus particle and it can deriver inclusions into cells via membrane-fusing activity. B-Liposome made of boronated phosphatide analogue can deliver the drug which is encapsulated into liposome with boron. We tried BNCT trail in vivo using an accelerator and new drugs. We used A549 cell line and OKTAVIAN which is 14 MeV neutron source by D-T reaction. In the result, these new boron agents realized the sufficient intracellular boron concentration and higher cytotoxicity reaction compared with a conventional boron compound (Sodium borocaptate: BSH). Therefore, we confirmed the possibility of BNCT using new drugs.

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“…3 Effects of BNCT on the Survival. The relation ship of equivalent dose and antitumor effects is similar to other reports 7,8) .…”

Section: Discussionsupporting

confidence: 85%

“…BNCT (Boron Neutron Capture Therapy) is a less-invasive tumor therapy based on the reaction between boron ( 10 B) and thermal neutron. 10 B captures thermal neutron efficiently and produce α and 7 Li ray. They have high reactive biological effectiveness (RBE) and the range of them is less than 10 μm in tissue 1) .…”

Section: Introductionmentioning

confidence: 99%

Basic investigation of boron neutron capture therapy (BNCT) using novel boron agents and accelerator based neutron source

et al. 2009

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“…This form of fludarabine has the ability to prevent the activity of enzymes involved in DNA synthesis and DNA repair. Inhibition of DNA polymerase alpha, DNA primase, ligase, and reductase induces DNA damage and arrest the cell cycle (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Combination of Fludarabine and Imatinib Induces Apoptosis Synergistically Through Loss of Mitochondrial Membrane Potential and Increases in Caspase-3 Enzyme Activity in Human K562 Chronic Myleloid Leukemia Cells

Baran

Oztekin

Bassoy

2010

Cancer Investigation

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In this study, we aimed to show the synergistic apoptotic effects of imatinib/fludarabine combination in human K562 chronic myleloid leukemia (CML) cells. There was a significant increase in cytotoxicity of combination of imatinib and fludarabine as compared to any agent alone. On the other hand, combination of both agents induced apoptosis significantly as confirmed by increases in caspase-3 enzyme activity and decreases in mitochondrial membrane potential. As a summary, the results of this study strongly suggest that combination of imatinib and fludarabine induced cell death synergistically comparing to only imatinib or fludarabine in human K562 CML cells.

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“…No effects were observed in any of the cell lines with EPA supplementation duration of 24 hours prior to irradiation. Treatment schedule-dependent results are also known for other drugs from many previous studies [6,26,35,40,42,49]. During EPA treatment 24 hours prior to irradiation instead of 30 minutes prior to irradiation, the cells have time to store the excess of potentially damaging nonesterified fatty acids in a safer neutral lipid form, such as triacylglycerol, in lipid droplets [20].…”

Section: Discussionmentioning

confidence: 94%

Omega-3 Fatty Acid Supplementation in Cancer Therapy

et al. 2011

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The combined effect of fludarabine monophosphate and radiation as well as gemcitabine and radiation on squamous carcinoma tumor cell linesin vitro

Abstract: Our results reveal a potential radiosensitizing effect of fludarabine and its possible application in chemoradiotherapy of advanced head and neck carcinoma and possibly other tumor entities.

Cited by 8 publications

References 38 publications

Basic investigation of boron neutron capture therapy (BNCT) using novel boron agents and accelerator based neutron source

Basic investigation of boron neutron capture therapy (BNCT) using novel boron agents and accelerator based neutron source

Combination of Fludarabine and Imatinib Induces Apoptosis Synergistically Through Loss of Mitochondrial Membrane Potential and Increases in Caspase-3 Enzyme Activity in Human K562 Chronic Myleloid Leukemia Cells

Omega-3 Fatty Acid Supplementation in Cancer Therapy

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