The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice

Tousen, Yuko; Ichimaru, Ryota; Kondo, Takashi; Inada, Masaki; Miyaura, Chisato; Ishimi, Yoshiko

doi:10.3390/nu12072043

Cited by 9 publications

(8 citation statements)

References 36 publications

(71 reference statements)

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“…In conclusion, the safety and efficiency of natural compounds have received attention in various diseases, including bone diseases [ 53 , 54 , 55 , 56 , 57 ]. Diet is also important in preventing the risk of osteoporosis and treating patients with osteoporosis [ 58 , 59 , 60 ]. In particular, isoflavone-rich cowpea has been reported to increase the proliferation and differentiation of human osteoblasts through the activation of the BMP2 pathway.…”

Section: Discussionmentioning

confidence: 99%

Calycosin-7-O-β-Glucoside Isolated from Astragalus membranaceus Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

Park

Kim

et al. 2021

IJMS

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Stem cells have received attention in various diseases, such as inflammatory, cancer, and bone diseases. Mesenchymal stem cells (MSCs) are multipotent stem cells that are critical for forming and repairing bone tissues. Herein, we isolated calycosin-7-O-β-glucoside (Caly) from the roots of Astragalus membranaceus, which is one of the most famous medicinal herbs, and investigated the osteogenic activities of Caly in MSCs. Caly did not affect cytotoxicity against MSCs, whereas Caly enhanced cell migration during the osteogenesis of MSCs. Caly increased the expression and enzymatic activities of ALP and the formation of mineralized nodules during the osteogenesis of MSCs. The osteogenesis and bone-forming activities of Caly are mediated by bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, Wnt3a, phospho-GSK3β, and phospho-AKT, inducing the expression of runt-related transcription factor 2 (RUNX2). In addition, Caly-mediated osteogenesis and RUNX2 expression were attenuated by noggin and wortmannin. Moreover, the effects were validated in pre-osteoblasts committed to the osteoblast lineages from MSCs. Overall, our results provide novel evidence that Caly stimulates osteoblast lineage commitment of MSCs by triggering RUNX2 expression, suggesting Caly as a potential anabolic drug to prevent bone diseases.

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Section: Discussionmentioning

confidence: 99%

Calycosin-7-O-β-Glucoside Isolated from Astragalus membranaceus Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

Park

Kim

et al. 2021

IJMS

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“…Isoflavones stimulate the activity and proliferation of osteoblasts [47] and inhibit bone resorption [26]. Isoflavones prevent bone resorption [58,59]. Isoflavones contribute to calcium homeostasis [70], and probiotic modulates the transcellular and paracellular pathways [46].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Tousen and colleagues [59] tested the combined effects of soy isoflavones (ISOs) and resveratrol (RES) on bone loss that was induced by hindlimb-unloading in mice. Female mice (ddY strain, 8 weeks) were randomly divided into six body weight-matched groups: a normally housed group (n = 6), a loading group (n = 6), a hindlimb-unloading group of mice that were fed a control diet (n = 6), aa hindlimb-unloading group of mice that were fed a 0.16% ISO conjugate diet (n = 8), a hindlimb-unloading group of mice that were fed a 0.15% RES diet (n = 8), and a hindlimb-unloading group of mice that were fed a 0.16% ISO conjugate and RES diet (n = 8).…”

Section: Genisteinmentioning

confidence: 99%

“…Shedd-Wise and colleagues [70] examined the three-year effects of soy isoflavones on BMD and strength in postmenopausal women. A double-blind, randomized controlled trial in 224 eligible women examined the effects of two soy isoflavone doses (80 or 120 mg/d) or placebo tablets on vBMD and strength (using peripheral quantitative computed tomography) in healthy postmenopausal women (age [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63]. The study found that isoflavone supplements' beneficial effects on the percentage change in the midshaft femur vBMD and on the midshaft femur strength-strain index improved for three years.…”

Section: Isoflavones Study Design Mechanism Findings Refmentioning

confidence: 99%

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Probiotics and Isoflavones as a Promising Therapeutic for Calcium Status and Bone Health: A Narrative Review

Harahap

Suliburska

2021

Foods

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Probiotics have potential clinical effects for treating and preventing osteoporosis. Meanwhile, isoflavones have attracted much attention due to their ability to prevent postmenopausal symptoms. Research has established that probiotics and isoflavones can regulate hormones, immune cells, and the gastrointestinal system, acting as links in the gut–bone axis. However, combining the effects of probiotics and isoflavones on calcium status and bone health is a more novel and a still-evolving research area. Lactobacillus and Bifidobacterium are the foremost strains that influence bone health to a significant extent. Among the isoflavones, daidzein, genistein, and the metabolites of genistein (such as equol) stimulate bone formation. It can be concluded that probiotics and isoflavones promote bone health by regulating calcium uptake, gut microbiota, and various metabolic pathways that are associated with osteoblast activity and bone formation. Nevertheless, further experiments of probiotics and isoflavones are still necessary to confirm the association between calcium bioavailability and bone health.

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“…A soy-based diet that contain isoflavones such as soy milk and Tofu is thought to contain estrogen-like compounds that may help inhibit bone loss and prevent osteoporosis because estrogen hormones help protect bone loss against postmenopausal osteoporosis [58]. It was reported that the combination of soy isoflavones and milk basic protein preserves bone mineral density, and the combination of resveratrol which is found in red wine, red grape skins, and peanuts also prevents bone loss [59,60]. In the present study, we demonstrated that PASI promotes osteoblast differentiation and mineralization.…”

Section: Discussionmentioning

confidence: 99%

Biological Mechanisms of Paeonoside in the Differentiation of Pre-Osteoblasts and the Formation of Mineralized Nodules

Park

Lee

Cho

et al. 2021

IJMS

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Paeonia suffruticosa is a magnificent and long-lived woody plant that has traditionally been used to treat various diseases including inflammatory, neurological, cancer, and cardiovascular diseases. In the present study, we demonstrated the biological mechanisms of paeonoside (PASI) isolated from the dried roots of P. suffruticosa in pre-osteoblasts. Herein, we found that PASI has no cytotoxic effects on pre-osteoblasts. Migration assay showed that PASI promoted wound healing and transmigration in osteoblast differentiation. PASI increased early osteoblast differentiation and mineralized nodule formation. In addition, PASI enhanced the expression of Wnt3a and bone morphogenetic protein 2 (BMP2) and activated their downstream molecules, Smad1/5/8 and β-catenin, leading to increases in runt-related transcription factor 2 (RUNX2) expression during osteoblast differentiation. Furthermore, PASI-mediated osteoblast differentiation was attenuated by inhibiting the BMP2 and Wnt3a pathways, which was accompanied by reduction in the expression of RUNX2 in the nucleus. Taken together, our findings provide evidence that PASI enhances osteoblast differentiation and mineralized nodules by regulating RUNX2 expression through the BMP2 and Wnt3a pathways, suggesting a potential role for PASI targeting osteoblasts to treat bone diseases including osteoporosis and periodontitis.

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The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice

Cited by 9 publications

References 36 publications

Calycosin-7-O-β-Glucoside Isolated from Astragalus membranaceus Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

Calycosin-7-O-β-Glucoside Isolated from Astragalus membranaceus Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells

Probiotics and Isoflavones as a Promising Therapeutic for Calcium Status and Bone Health: A Narrative Review

Biological Mechanisms of Paeonoside in the Differentiation of Pre-Osteoblasts and the Formation of Mineralized Nodules

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