The combination of multiple plant growth promotion and hydrolytic enzyme producing rhizobacteria and their effect on Jerusalem artichoke growth improvement

Abstract

Rhizobacteria are well recognized for their beneficial multifunctions as key promoters of plant development, suppressing pathogens, and improving soil health. In this study, experiments focused on characterizing the plant growth promotion (PGP) and extracellular hydrolase production traits of rhizobacteria, and their impact on Jerusalem artichoke growth. A total of 50 isolates proved capable of either direct PGP or hydrolase-producing traits. Two promising strains (Enterobactercloacae S81 and Pseudomonasazotoformans C2-114) showed potential on phosphate and potassium solubilization, IAA production, and 1-aminocyclopropane-1-carboxylic acid deaminase activity and hydrolase production. A hydrolase-producing strain (Bacillussubtilis S42) was able to generate cellulase, protease, amylase, β-glucosidase, and phosphatase. These three selected strains also gave positive results for indirect PGP traits such as siderophore, ammonia, oxalate oxidase, polyamine, exopolysaccharide, biofilm, motility, and tolerance to salinity and drought stress. Colonization was observed using a scanning electron microscope and rhizobacteria appeared at the root surface. Interestingly, inoculation with consortia strains (S42, S81, and C2-114) significantly increased all plant parameters, including height, biomass, root (length, surface, diameter, and volume), and tuber fresh weight. Therefore, we recommend that potential consortia of PGP and hydrolase-producing rhizobacteria be employed as a biofertilizer to improve soil and boost crop productivity.

Optimizing tomato seedling growth with indigenous mangrove bacterial inoculants and reduced NPK fertilization

“…Our results revealed that plants treated with the mix of native PGP strains and 50%NPK had considerably higher contents of N, P, K, Ca, Fe, and Zn ( Table 7 ). Our results aligned with several previous research ( Ye et al., 2020 ; Scagliola et al., 2021 ; Sritongon et al., 2023 ). A theoretical relationship between the in vitro and in vivo results could explain these findings.…”

Characterization and evaluation of Bacillus subtilis GYUN-2311 as a biocontrol agent against Colletotrichum spp. on apple and hot pepper in Korea

“…Furthermore, the synthesis of additional enzymes, such as amylase ( Dai et al, 2020 ), protease and cellulase by Bacillus spp. may contribute to the suppression of diseases caused by pathogenic fungus ( Sritongon et al, 2023 ). Chitinases produced by B .…”

“…Furthermore, the synthesis of additional enzymes, such as amylase (Dai et al, 2020), protease and cellulase by Bacillus spp. may contribute to the suppression of diseases caused by pathogenic fungus (Sritongon et al, 2023). Chitinases produced by B. subtilis have antifungal properties and can degrade the cell walls of several pathogenic fungi, including R. solani (Saber et al, 2015).…”

Identification of Growth-Promoting Bacterial Resources by Investigating the Microbial Community Composition of Polyporus umbellatus Sclerotia

“…Acinetobacter and Pseudomonas belong to the phylum Proteobacteria, and their frequent co-occurring interactions with bacteria may contribute to the high yield of P. umbellatus. In a previous study, a mixture of three isolates ( Enterobacter cloacae , Pseudomonas azotoformans , and Bacillus subtilis ) enhanced the biomass and size of Jerusalem artichoke tubers of host plants [ 35 ]. Moreover, the simultaneous fermentation of Bacillus subtilis and Trichoderma harzianum enhances plant growth, whereas the co-fermentation of Rhizopus nigricans and Trichoderma pseudokoningii synergistically controls Fusarium oxysporum [ 36 ].…”