The combination of four main components in Xuebijing injection improved the preventive effects of Cyclosporin A in acute graft-versus-host disease mice by protecting intestinal microenvironment

Shang, Tao; Guo, Yue; Li, Xiurong; Zhou, Zhengcan; Qi, Yubo; Salahdiin, Khalid; Shen, Ru; He, Shuang; Wang, Mei; Z, Shi; Zhao, Xin; Yang, Jian; Fan, Guanwei; Wang, Yuefei; Gao, Xiumei; Zhu, Yan; Feng, Yi

doi:10.1016/j.biopha.2022.112675

Cited by 6 publications

(5 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Real-time PCR experiments were performed as previously described ( Shang et al, 2022 ). The total RNA was extracted using Trizol, including lysing cells, isolating, precipitating, and washing RNA.…”

Section: Methodsmentioning

confidence: 99%

ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway

Peng,

Lv,

Wang

et al. 2023

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Sepsis is a life-threatening multiple organ dysfunction syndrome (MODS) caused by a microbial infection that leads to high morbidity and mortality worldwide. Sepsis-induced cardiomyopathy (SIC) and coagulopathy promote the progression of adverse outcomes in sepsis. Here, we reported that ACT001, a modified compound of parthenolide, improved the survival of sepsis mice. In this work, we used cecal ligation and puncture (CLP) model to induce SIC. Transthoracic echocardiography and HE staining assays were adopted to evaluate the influence of ACT001 on sepsis-induced cardiac dysfunction. Our results showed that ACT001 significantly improved heart function and reduced SIC. Coagulation accelerates organ damage in sepsis. We found that ACT001 decreased blood clotting in the FeCl3-induced carotid artery thrombosis experiment. ACT001 also reduced the production of neutrophil extracellular traps (NETs). RNA-sequencing of heart tissues revealed that ACT001 significantly downregulated the expression of pro-inflammatory cytokines and the JAK-STAT signaling pathway. These results were confirmed with real-time PCR and ELISA. In summary, we found ACT001 rescued mice from septic shock by protecting the cardiovascular system. This was partially mediated by inhibiting pro-inflammatory cytokine production and down-regulating the JAK-STAT signaling.

show abstract

Section: Methodsmentioning

confidence: 99%

ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway

Peng,

Lv,

Wang

et al. 2023

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…After 1 min in dark, the EasySee Western Blot Kit (DW101-02, TransGen Biotech, Beijing, China) reacted with the PVDF membrane, and the bands were observed via the gel imaging system. The proteins expression levels of CitH3, CXCR-2, caspase-11, GSDMD and MPO were analyzed using ImageJ software and standardized to GAPDH ( Shang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the sections were sealed with neutral gum. The expressions of NE and CitH3 were detected with an optical microscope (Vectra 3) and quantified by ImageJ software ( Shang et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Xuebijing injection inhibited neutrophil extracellular traps to reverse lung injury in sepsis mice via reducing Gasdermin D

Shang

Zhang²,

Wang³

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

The mortality of sepsis and septic shock remains high worldwide. Neutrophil extracellular traps (NETs) release is a major cause of organ failure and mortality in sepsis. Targeting Gasdermin D (GSDMD) can restrain NETs formation, which is promising for sepsis management. However, no medicine is identified without severe safety concerns for this purpose. Xuebijing injection (XBJ) has been demonstrated to alleviate the clinical symptoms of COVID-19 and sepsis patients, but there are not enough animal studies to reveal its mechanisms in depth. Therefore, we wondered whether XBJ relieved pulmonary damage in sepsis by suppressing NETs formation and adopted a clinically relevant polymicrobial infection model to test this hypothesis. Firstly, XBJ effectively reversed lung injury caused by sepsis and restrained neutrophils recruitment to lung by down-regulating proinflammatory chemokines, such as CSF-3, CXCL-2, and CXCR-2. Strikingly, we found that XBJ significantly reduced the expressions of NETs component proteins, including citrullinated histone H3 (CitH3), myeloperoxidase (MPO), and neutrophil elastase (NE). GSDMD contributes to the production of NETs in sepsis. Notably, XBJ exhibited a reduced effect on the expressions of GSDMD and its upstream regulators. Besides, we also revealed that XBJ reversed NETs formation by inhibiting the expressions of GSDMD-related genes. Collectively, we demonstrated XBJ protected against sepsis-induced lung injury by reversing GSDMD-related pathway to inhibit NETs formation.

show abstract

“…The quality of library was evaluated on the Qubit@ 2.0 Fluorometer (Thermo Scientific). Eventually, the library was sequenced on an Ion S5TM XL platform [23].…”

Section: High-throughput 16s Rrna Sequencingmentioning

confidence: 99%

HPV-associated cervicovaginal microbiome and host metabolome characteristics

Zhang,

Wu,

et al. 2024

BMC Microbiol

View full text Add to dashboard Cite

Background Cervicovaginal microbiome plays an important role in the persistence of HPV infection and subsequent disease development. However, cervicovaginal microbiota varied cross populations with different habits and regions. Identification of population-specific biomarkers from cervicovaginal microbiota and host metabolome axis may support early detection or surveillance of HPV-induced cervical disease at all sites. Therefore, in the present study, to identify HPV-specific biomarkers, cervicovaginal secretion and serum samples from HPV-infected patients (HPV group, n = 25) and normal controls (normal group, n = 17) in Xichang, China were collected for microbiome (16S rRNA gene sequencing) and metabolome (UHPLC-MS/MS) analysis, respectively. Results The results showed that key altered metabolites of 9,10-DiHOME, α-linolenic acid, ethylparaben, glycocholic acid, pipecolic acid, and 9,12,13-trihydroxy-10(E),15(Z)-octadecadienoic acid, correlating with Sneathia (Sneathia_amnii), Lactobacillus (Lactobacillus_iners), Atopobium, Mycoplasma, and Gardnerella, may be potential biomarkers of HPV infection. Conclusion The results of current study would help to reveal the association of changes in cervicovaginal microbiota and serum metabolome with HPV infections.

show abstract

The combination of four main components in Xuebijing injection improved the preventive effects of Cyclosporin A in acute graft-versus-host disease mice by protecting intestinal microenvironment

Cited by 6 publications

References 57 publications

ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway

ACT001 improved cardiovascular function in septic mice by inhibiting the production of proinflammatory cytokines and the expression of JAK-STAT signaling pathway

Xuebijing injection inhibited neutrophil extracellular traps to reverse lung injury in sepsis mice via reducing Gasdermin D

HPV-associated cervicovaginal microbiome and host metabolome characteristics

Contact Info

Product

Resources

About