The combination of four analytical methods to explore skeletal muscle metabolomics: Better coverage of metabolic pathways or a marketing argument?

Bruno, Clément; Patin, Franck; Bocca, Cinzia; Nadal-Desbarats, Lydie; Bonnier, Franck; Reynier, Pascal; Emond, Patrick; Vourc’h, Patrick; Joseph-Delafont, K.; Corcia, Philippe; Andres, Christian R.; Blasco, Hélène

doi:10.1016/j.jpba.2017.10.013

Cited by 27 publications

(24 citation statements)

References 40 publications

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“…An acute submaximal exercise bout led to significant increases (from rest) in plasma indexes of incomplete fatty acid (medium-chain acylcarnitines) oxidation (FAO) (46), in line with other reports (16,24). Other studies have examined broader exercise-associated metabolite patterns through application of metabolomics in blood (8,9,16,25,29,(31)(32)(33)(34)(35)(36) or in a limited number of muscle biopsy studies (7,12). These reports provide insight into the large muscle-and body-wide metabolic shifts that occur when muscle work is engaged.…”

Section: Introductionsupporting

confidence: 72%

Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans

Zhang

Bhattacharyya

Hickner

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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Zhang J, Bhattacharyya S, Hickner RC, Light AR, Lambert CJ, Gale BK, Fiehn O, Adams SH. Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans. Blood or biopsies are often used to characterize metabolites that are modulated by exercising muscle. However, blood has inputs derived from multiple tissues, biopsies cannot discriminate between secreted and intracellular metabolites, and their invasive nature is challenging for frequent collections in sensitive populations (e.g., children and pregnant women). Thus, minimally invasive approaches to interstitial fluid (IF) metabolomics would be valuable. A catheter was designed to collect IF from the gastrocnemius muscle of acutely anesthetized adult male rats at rest or immediately following 20 min of exercise (~60% of maximal O 2 uptake). Nontargeted, gas chromatography-time-of-flight mass spectrometry analysis was used to detect 299 metabolites, including nonannotated metabolites, sugars, fatty acids, amino acids, and purine metabolites and derivatives. Just 43% of all detected metabolites were common to IF and blood plasma, and only 20% of exercise-modified metabolites were shared in both pools, highlighting that the blood does not fully reflect the metabolic outcomes in muscle. Notable exercise patterns included increased IF amino acids (except leucine and isoleucine), increased ␣-ketoglutarate and citrate (which may reflect tricarboxylic acid cataplerosis or shifts in nonmitochondrial pathways), and higher concentration of the signaling lipid oleamide. A preliminary study of human muscle IF was conducted using a 20-kDa microdialysis catheter placed in the vastus lateralis of five healthy adults at rest and during exercise (65% of estimated maximal heart rate). Approximately 70% of commonly detected metabolites discriminating rest vs. exercise in rats were also changed in exercising humans. Interstitium metabolomics may aid in the identification of molecules that signal muscle work (e.g., exertion and fatigue) and muscle health. beta-oxidation; interstitium; metabolome

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Section: Introductionsupporting

confidence: 72%

Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans

Zhang

Bhattacharyya

Hickner

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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“…Muscle metabolomics. Total hydrophilic metabolite content was extracted from quadriceps muscle tissue at treatment endpoint through methanol/water (80:20) extraction, adapting conditions described previously (50). Briefly, total metabolite content from quadriceps muscle was obtained from about 100 mg (wet weight) quadriceps muscle tissue per animal.…”

Section: Supplemental Methods Are Available Online With This Articlementioning

confidence: 99%

Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming

Quattrocelli¹,

Zelikovich²,

Jiang³

et al. 2019

JCI Insight

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“…Metabolomics is a technique which can be utilised to analyse the metabolome of a cell or microorganism. This technique has been used to analyse the metabolomes of many microbes [ 7 , 8 ], plants [ 9 ], nematodes [ 10 ] and animal cells [ 11 , 12 , 13 ]. Additionally, it has also been used to detect the molecules present in biological liquids such as blood [ 14 ], urine [ 14 , 15 , 16 ] and breast milk [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…1 H NMR spectra have a proven track record for metabolite analysis from a number of biofluids and extraction methods [ 10 , 11 , 12 , 13 , 14 , 15 , 17 ]. Therefore, a combination of 1 H NMR metabolomics using a 1D- 1 H-NOESY pulse sequence with an extraction protocol that only extracts small molecules provides an effective method for mapping Blastocystis metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

Establishing a Metabolite Extraction Method to Study the Metabolome of Blastocystis Using NMR

et al. 2021

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Blastocystis is an opportunistic parasite commonly found in the intestines of humans and other animals. Despite its high prevalence, knowledge regarding Blastocystis biology within and outside the host is limited. Analysis of the metabolites produced by this anaerobe could provide insights that can help map its metabolism and determine its role in both health and disease. Due to its controversial pathogenicity, these metabolites could define its deterministic role in microbiome’s “health” and/or subsequently resolve Blastocystis’ potential impact in gastrointestinal health. A common method for elucidating the presence of these metabolites is through 1H nuclear magnetic resonance (NMR). However, there are currently no described benchmarked methods available to extract metabolites from Blastocystis for 1H NMR analysis. Herein, several extraction solvents, lysis methods and incubation temperatures were compared for their usefulness as an extraction protocol for this protozoan. Following extraction, the samples were freeze-dried, re-solubilized and analysed with 1H NMR. The results demonstrate that carrying out the procedure at room temperature using methanol as an extraction solvent and bead bashing as a lysis technique provides a consistent, reproducible and efficient method to extract metabolites from Blastocystis for NMR.

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The combination of four analytical methods to explore skeletal muscle metabolomics: Better coverage of metabolic pathways or a marketing argument?

Cited by 27 publications

References 40 publications

Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans

Skeletal muscle interstitial fluid metabolomics at rest and associated with an exercise bout: application in rats and humans

Pulsed glucocorticoids enhance dystrophic muscle performance through epigenetic-metabolic reprogramming

Establishing a Metabolite Extraction Method to Study the Metabolome of Blastocystis Using NMR

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