The combination of ezetimibe and ursodiol promotes fecal sterol excretion and reveals a G5G8-independent pathway for cholesterol elimination

Wang, Yuhuan; Liu, Xiaoxi; Pijut, Sonja S.; Li, Jianing; Horn, Jamie; Bradford, Emily M.; Leggas, Markos; Barrett, Terrence A.; Graf, Gregory A.

doi:10.1194/jlr.m053454

Cited by 14 publications

(13 citation statements)

References 40 publications

(51 reference statements)

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“…In support of this, Wang et al showed recently that feeding ABCG5/G8 double knock-out mice with ursodeoxycholate stimulated FNS excretion considerably. 35 As suggested by Le May et al 5 , the multidrug resistance transporter ABCB1 may be responsible for the ABCG5/G8-independent TICE flux or a part thereof. We did not detect increased intestinal expression of the Abcb1a and b genes under any of the stimulated conditions (data not shown).…”

Section: Discussionmentioning

confidence: 96%

Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice

et al. 2017

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Section: Discussionmentioning

confidence: 96%

Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice

et al. 2017

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“…Animal Studies: Plasma, basal bile, urine, and livers were collected from wild-type (WT) and SR-BIdeficient (B6;129S2-Scarb1tm1Kri/J, SR-BI KO) mice, as previously described 57 . Mice were anesthetized with urethane (1g/kg, i.p.).…”

Section: Methodsmentioning

confidence: 99%

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Allen

Zhao

Ramirez‐Solano

et al. 2018

Preprint

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To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), we developed a novel pipeline to overcome current limitations in analysis entitled, “Tools for Integrative Genome analysis of Extracellular sRNAs (TIGER)”. To demonstrate the power of this tool, sRNA-seq was performed on mouse lipoproteins, bile, urine, and liver samples. A key advance for the TIGER pipeline is the ability to analyze both host and non-host sRNAs at genomic, parent RNA, and individual fragment levels. TIGER was able to identify approximately 60% of sRNAs on lipoproteins, and >85% of sRNAs in liver, bile, and urine, a significant advance compared to existing software. Results suggest that the majority of sRNAs on lipoproteins are non-host sRNAs derived from bacterial sources in the microbiome and environment, specifically rRNA-derived sRNAs from Proteobacteria. Collectively, TIGER facilitated novel discoveries of lipoprotein and biofluid sRNAs and has tremendous applicability for the field of extracellular RNA.

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“…Plasma, basal bile, urine and livers were collected from wild-type (WT) and SR-BI-deficient ( B6;129S2-Scarb1tm1Kri/J , SR-BI KO) mice, as previously described [14]. Mice were anaesthetized with urethane (1g/kg, i.p.).…”

Section: Methodsmentioning

confidence: 99%

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Allen

Zhao

Solano

et al. 2018

J of Extracellular Vesicle

Self Cite

View full text Add to dashboard Cite

To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), we developed a novel pipeline to overcome current limitations in analysis entitled, “Tools for Integrative Genome analysis of Extracellular sRNAs (TIGER)”. To demonstrate the power of this tool, sRNA-seq was performed on mouse lipoproteins, bile, urine and livers. A key advance for the TIGER pipeline is the ability to analyse both host and non-host sRNAs at genomic, parent RNA and individual fragment levels. TIGER was able to identify approximately 60% of sRNAs on lipoproteins and >85% of sRNAs in liver, bile and urine, a significant advance compared to existing software. Moreover, TIGER facilitated the comparison of lipoprotein sRNA signatures to disparate sample types at each level using hierarchical clustering, correlations, beta-dispersions, principal coordinate analysis and permutational multivariate analysis of variance. TIGER analysis was also used to quantify distinct features of exRNAs, including 5ʹ miRNA variants, 3ʹ miRNA non-templated additions and parent RNA positional coverage. Results suggest that the majority of sRNAs on lipoproteins are non-host sRNAs derived from bacterial sources in the microbiome and environment, specifically rRNA-derived sRNAs from Proteobacteria. Collectively, TIGER facilitated novel discoveries of lipoprotein and biofluid sRNAs and has tremendous applicability for the field of extracellular RNA.

show abstract

The combination of ezetimibe and ursodiol promotes fecal sterol excretion and reveals a G5G8-independent pathway for cholesterol elimination

Cited by 14 publications

References 40 publications

Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice

Intestinal Farnesoid X Receptor Controls Transintestinal Cholesterol Excretion in Mice

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

Bioinformatic analysis of endogenous and exogenous small RNAs on lipoproteins

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