The colorimetric estimation of pyrogallol, gallotannin and gallic acid

Mitchell, C. Ainsworth

doi:10.1039/an9234800002

Cited by 29 publications

(5 citation statements)

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“…Violet darkness sediment (http://bbs.bioon.net/bbs/home. php?mod=space&uid=205516&do=blog&quickforward =1&id=99320) in the bottom tube by centrification of the treated cells, which confirmed that catechins or GA monomer had been entered into the cytoplasm and further chelated [42] with the Fe 2+/3+ of LIP. However, we are not sure whether catechins or GA monomer entered into cellular through passive or active transport pathway.…”

Section: Fig 10 Schematic Diagram Of Rko Cells Apoptosis or Death Insupporting

confidence: 58%

Apoptosis of RKO induced by catechins and GA through Ca2+ and LIP

2014

Wuhan Univ. J. Nat. Sci.

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The anti-tumor effects of catechins and gallic acid (GA) in-vitro was investigated in this paper. Fluo-3AM, Calcium-AM (Ca-AM), 2′ , 7′ -dichlorofluorescein-diacetate(DCFH-DA), 4′ , 6-diamidino-2-phenylindole (DAPI) and Ca-AM plus colbat were used to characterize intracellular calcium, labile iron pool (LIP), reactive oxygen species (ROS), nuclei morphology and mitochondrial permeability transition pore (mPTP) opening, respectively. High performance liquid chromatography (HPLC) was used to quantitate catechins and GA in the cultural medium. The results indicated that each of them showed dose response inhibition of cell growth, provoking nuclei condensation, intracellular calcium elevation, mPTP opening, LIP reduction, and cytochrome c (Cyt-C) to release into cytosol. The caspase inhibitors, 2-aminoethoxydiphenol borate (APB) or Fe 3+ could inhibit lethal effects of GA and (-)-epigallocatechin (EGC), but failed to affect (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG). Level of ROS presented negative growth while their concentration decreased in the medium. In conclusion, our findings suggest that viability of RKO decreased because of their good correlation with elevation of calcium and loss of LIP and ROS in cytosol.

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Section: Fig 10 Schematic Diagram Of Rko Cells Apoptosis or Death Insupporting

confidence: 58%

Apoptosis of RKO induced by catechins and GA through Ca2+ and LIP

2014

Wuhan Univ. J. Nat. Sci.

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“…Based on the original proposal by Mitchell (1923), essentially a specialized version of the later Jerumanis-type reaction can be used to estimate pyrogallol substitution patterns in the presence of catechol rings (King and White 1956). The regulation of pH is critical.…”

Section: Inorganic Complex Colorimetrymentioning

confidence: 99%

Wine Phenols

Singleton¹

1988

Modern Methods of Plant Analysis

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“…Only one method for the determination of tannic acid was found which could be modified for use in the presence of nordihydroguaiaretic acid. The reaction was described by Mitchell (7,9) and involved the formation of a violet color on mixing a buffered solution of tannic acid and a ferrous sulfate-sodium potassium tartrate reagent. It was sensitive to concentrations as low as 20 micrograms per ml.…”

Section: Estimation Of Possible Interfering Compoundsmentioning

confidence: 99%