The Collagens and Their Urologic Implications

Macarak, Edward J.; Ewalt, David H.; Baskin, Lawrence; Coplen, D.E.; Koo, Harry P.; Levin, Robert M.; Duckett, John W.; Snyder, Howard M.; Rosenbloom, Joel; Howard, Pamela S.

doi:10.1007/978-1-4899-1585-6_20

Cited by 23 publications

(23 citation statements)

References 18 publications

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“…Elastin and the elastinassociated microfibrillar proteins, fibrillin-1 and MAGP, are also associated with both compartments of the bladder wall, and are thought to contribute flexibility and elasticity ]. The proper arrangement of these matrix proteins with one another and with the cellular components of the bladder wall all contribute to normal organ function: gradual expansion of the bladder wall on filling and rapid contraction and recoil during micturition [Macarak and Howard, 1997;Chang et al, 1998]. Quantitative changes in bladder ECM composition as well as alterations in the arrangement of matrix proteins can contribute to loss of bladder function and ultimately may lead to renal impairment [Lin and McConnell, 1995;Shapiro and Lepor, 1995;Macarak and Howard, 1997].…”

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confidence: 99%

“…The proper arrangement of these matrix proteins with one another and with the cellular components of the bladder wall all contribute to normal organ function: gradual expansion of the bladder wall on filling and rapid contraction and recoil during micturition [Macarak and Howard, 1997;Chang et al, 1998]. Quantitative changes in bladder ECM composition as well as alterations in the arrangement of matrix proteins can contribute to loss of bladder function and ultimately may lead to renal impairment [Lin and McConnell, 1995;Shapiro and Lepor, 1995;Macarak and Howard, 1997]. Although significant progress has been made in identifying and characterizing the major components of the bladder ECM, comparatively little is known about the "bridging molecules" that interconnect specific components of the ECM with each other and resident cells.…”

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confidence: 99%

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Extracellular matrix and nuclear localization of βig‐h3 in human bladder smooth muscle and fibroblast cells

Billings

Herrick

Kucich

et al. 2000

J of Cellular Biochemistry

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The extracellular matrix (ECM) plays an essential role in bladder structure and function. In this study, expression of beta ig-h3, a recently identified extracellular matrix protein, was investigated in human bladder tissue, and human bladder smooth-muscle (SMC) and fibroblast cells in vitro. SMCs secreted greater than three times the level of this protein compared with fibroblasts. The relative levels of beta ig-h3 mRNA in the two cell types reflected the protein expression. Immunohistochemical analysis demonstrated protein deposition in the ECM as well as cytoplasmic localization and, unexpectedly, nuclei. Anti-beta ig-h3 antibodies also stained the matrix surrounding the detrusor SMCs and nuclei of bladder fibroblasts, SMCs, and urothelium in intact bladder tissue. Western blot analyses of medium and matrix fractions obtained from cells in vitro revealed protein of approximately 70-74 kDa, whereas nuclear extracts contained a 65-kDa reactive protein band. We propose that although this protein is a structural component of bladder ECM, its nuclear localization suggests that it has other regulatory and/or structural functions.

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confidence: 99%

Extracellular matrix and nuclear localization of βig‐h3 in human bladder smooth muscle and fibroblast cells

Billings

Herrick

Kucich

et al. 2000

J of Cellular Biochemistry

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“…ECMs were similar in both equivalents; they were thicker, better and more comparable to those of the bladder with BSCs equivalents. 28 Our results showed that both equivalents had a wellstratified urothelium. However, terminal differentiation of urothelium was obtained only with BSCs-based equivalents, as CK20 is specific for umbrella cells in normal bladder and the marker of final differentiation for superficial cells.…”

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confidence: 49%

Optimization of the current self-assembled urinary bladder model: Organ-specific stroma and smooth muscle inclusion

Orabi

Rousseau

Laterreur

et al. 2015

CUAJ

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Introduction: Due to the complications associated with the use of non-native biomaterials and the lack of local tissues, bioengineered tissues are required for surgical reconstruction of complex urinary tract diseases, including those of the urinary bladder. The selfassembly method of matrix formation using autologous stromal cells obviates the need for exogenous biomaterials. We aimed at creating novel ex-vivo multilayer urinary tissue from a single bladder biopsy. Methods: After isolating urothelial, bladder stromal and smooth muscle cells from bladder biopsies, we produced 2 models of urinary equivalents: (1) the original one with dermal fibroblasts and (2) the new one with bladder stromal cells. Dermal fibroblasts and bladder stromal cells were stimulated to form an extracellular matrix, followed by sequential seeding of smooth muscle cells and urothelial cells. Stratification and cellular differentiation were assessed by histology, immunostaining and electron microscopy. Barrier function was checked with the permeability test. Biomechanical properties were assessed with uniaxinal tensile strength, elastic modulus, and failure strain. Results: Both urinary equivalents could be handled easily and did not contract. Stratified epithelium, intact basement membrane, fused matrix, and prominent muscle layer were detected in both urinary equivalents. Bladder stromal cell-based constructs had terminally differentiated urothelium and more elasticity than dermal fibroblasts-based equivalents. Permeation studies showed that both equivalents were comparable to native tissues. Conclusions: Organ-specific stromal cells produced urinary tissues with more terminally differentiated urothelium and better biomechanical characteristics than non-specific stromal cells. Smooth muscle cells could be incorporated into the selfassembled tissues effectively. This multi-layer tissue can be used as a urethral graft or as a bladder model for disease modelling and pharmacotherapeutic testing.

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“…Many studies demonstrated that in BOO there is recruitment of neural-detrusor responses causing altered neural control of micturition resulting in reduced bladder contractility, impaired central processing, and altered sensation [28][29][30][31][32]. Gosling et al [9] in a prospective study showed that BOO leads to decrease in the autonomic innervations of human DM which in turn results in decrease sensitivity of bladder.…”

Section: Neurological Diseasementioning

confidence: 99%

Does Temporary Catheter Drainage of Urine Improves Detrusor Function in Chronic Urinary Retention in Patients with Bladder Outlet Obstruction.

Pal¹

2017

UNOAJ

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Chronic urinary retention (CUR) is one of the consequences of bladder outlet obstruction (BOO) that occurs due to impaired functions of detrusor muscles secondary to obstruction related changes in the bladder wall. The effect of catheter drainage (CD) on detrusor function in CUR in animals is well studied but in humans there are very few studies to see its effect in CUR. Material and methods:This is a non-randomized, retrospective study wherein case records of 50 eligible patients attending the Department of Urology during January 2010 to March 2012 were evaluated for CUR. Two UDS, performed 6-12 weeks (average 9 weeks) apart and the parameters which were evaluated were bladder sensation, capacity and voiding detrusor pressure. These parameters were also evaluated against the age of the patient, duration of symptoms and the amount of urine drained on catheterization. Results:The commonest symptoms observed were decrease flows of urine, frequency, straining and incomplete emptying. Bladder function improvement after CD was observed to be maximum in patients with following characteristics: age at presentation in the range of > 60 to < 70 years, duration of lower urinary tract symptoms (LUTS) less than 5 years and amount of urine drained at the time of catheterization being < 500 ml. Conclusion:CUR secondary to BOO is a common problem in elderly males. UDS is the "gold standard" for its evaluation and helps to plan further management. CD of urine is an effective mode of temporary management in patients of CUR secondary to BOO as it provides rest to the detrusor muscle in humans.

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The Collagens and Their Urologic Implications

Cited by 23 publications

References 18 publications

Extracellular matrix and nuclear localization of βig‐h3 in human bladder smooth muscle and fibroblast cells

Extracellular matrix and nuclear localization of βig‐h3 in human bladder smooth muscle and fibroblast cells

Optimization of the current self-assembled urinary bladder model: Organ-specific stroma and smooth muscle inclusion

Does Temporary Catheter Drainage of Urine Improves Detrusor Function in Chronic Urinary Retention in Patients with Bladder Outlet Obstruction.

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