The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes

Abstract

Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method.

Development and characterization of poultry collagen-based hybrid hydrogels for bone regeneration

“…Biodegradation of materials occurs through the action of inflammatory infiltrate cells that fragment and phagocytose the product, being a very important process, as it favors tissue regeneration in the area previously occupied by the implant 2 , 40 - 42 . The fibrous architecture and crosslinking of biomaterials from different xenogeneic origins can explain different rates of biodegradation, as observed in an assay with enzymatic action of trypsin and collagenase in up to 50 days, in which equine collagen products exhibited greater biodegradation than swine and bovine 46 .…”

“…The results observed for bone neoformation of all treatments at one and three months converge with results from thematic studies using critical size defect model in rat calvaria. Polymers such as collagen, poly(lactic-co-glycolic acid) or demineralized bone matrices induced 3 to 10% bone neoformation between two and 12 weeks 20 , 46 , 47 . For ceramic materials (HA, β-TCP, β-TCMP) or bioglasses, of different formulations, the percentage of bone neoformation shows a wide variation, from 1.8 to 25% between two to 12 weeks post-implantation 48 - 50 .…”

Antibiotic-Loaded Polymeric Barrier Membranes for Guided Bone/Tissue Regeneration: A Mini-Review

“…Using the National Library of Medicine (MEDLINE by PubMed), The Cochrane Oral Health Group Trials Register, EMBASE, and Web of Science (WOS) a literature search was performed for papers, including peer-reviewed publications from 1963 up to January 2022. The estimated healing period in bone regeneration is more than 6 months, and for periodontal regeneration, 4 to 6 weeks are necessary [11]. Antibacterials have shorter lifespans and rapid local clearances at bone healing sites.…”

“…The combination of polymeric barrier membranes and antibacterials are preferred in order to facilitate, accelerate, and enhance the effect of guided tissue and guided bone regeneration procedures [4,7]. The estimated healing period in bone regeneration is more than 6 months, and for periodontal regeneration, 4 to 6 weeks are necessary [11]. Antibacterials have shorter lifespans and rapid local clearances at bone healing sites.…”

“…The estimated healing period in bone regeneration is more than 6 months, and for periodontal regeneration, 4 to 6 weeks are necessary [ 11 ]. Antibacterials have shorter lifespans and rapid local clearances at bone healing sites.…”