The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke

Abstract

Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans, but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke.

Emerging Evidence for Pathogenesis of Sporadic Cerebral Small Vessel Disease

“…46 Consistent with these data, a recent populationbased study showed a strong correlation between cerebral microbleeds and collagen-binding proteins-positive S. mutans with a significantly high odds ratio for cerebral microbleeds at 14.4. 51 Another dental bacterium, Porphyromonas gingivalis, is also found in atherosclerotic plaques and has been linked to the increased risk of ischemic stroke.…”

“…Из всех известных патогенных бакте-рий ротовой полости, только немногие были связаны с цереброваскулярной болезнью [45,46]. K. Nakano и соавт.…”

“…K. Nakano и соавт. [46] недавно продемонстрировали, что некото-рые штаммы Streptococcus mutans являются потенциаль-ными факторами риска развития внутримозгового кро-воизлияние у КСГПИ и мышей с фотохимически инду-цированной окклюзией средней мозговой артерии. Это соответствует результатам, показывающим, что инфек-ции пародонта являются факторами риска развития инсульта [47,48] и что S. mutans обнаруживается в 100% образцов атеросклеротических бляшек [49].…”

“…44 Of all the known pathogenic oral bacteria, a few have been linked to cerebrovascular diseases. 45,46 Nakano et al 46 recently demonstrated that certain strains of Streptococcus mutans are potential risk factors for intracerebral hemorrhage in SHR-SPs and mice with photochemically induced middle cerebral artery occlusion. This corresponds with findings showing periodontal infections to be risk factors for stroke, 47,48 and that S. mutans is detected in 100% of samples of atherosclerotic plaques.…”

Longitudinal comparison of Streptococcus mutans-induced aggravation of non-alcoholic steatohepatitis in mice

“…Cnm is associated with the severity of infective endocarditis due to its modulation of the adhesive and invasive properties of the bacteria for blood vessel endothelial cells [6,7]. Furthermore, Cnm-positive S. mutans is associated with aggravation of cerebral haemorrhage and inflammatory bowel disease [8,9]. …”