The coiled coils in the design of protein-based constructs: hybrid hydrogels and epitope displays

Tang, Aaron; Wang, Chun; Stewart, Russell J.; Kopeček, Jindřich

doi:10.1016/s0168-3659(01)00262-0

Cited by 53 publications

(42 citation statements)

References 39 publications

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“…The coiled coil is one of the basic folding patterns of native proteins, consisting of two or more right-handed amphiphilic α-helices wound together noncovalently to form a slightly left-handed superhelix ( Figure 15) [176][177][178]. The coiled coil domain is based on a heptad repeat unit abcdefg, where a and d are typically hydrophobic amino acids (leucine), and e and g are charged (glutamic acid) [179].…”

Section: Coiled Coil Domainsmentioning

confidence: 99%

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Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

280

231

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Peptides are emerging as a new class of biomaterials due to their unique chemical, physical, and biological properties. The development of peptide-based biomaterials is driven by the convergence of protein engineering and macromolecular self-assembly. This review covers the basic principles, applications, and prospects of peptide-based biomaterials. We focus on both chemically synthesized and genetically encoded peptides, including poly-amino acids, elastin-like polypeptides, silk-like polymers and other biopolymers based on repetitive peptide motifs. Applications of these engineered biomolecules in protein purification, controlled drug delivery, tissue engineering, and biosurface engineering are discussed.

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Section: Coiled Coil Domainsmentioning

confidence: 99%

“…This behavior has been exploited to create pH-sensitive and temperature-sensitive hydrogels [176,181]. These genetically encoded hydrogels demonstrated tunable diffusion, which is controlled by the residues composing the heptad sequence as well as the state of the hydrogel (soluble versus collapsed) [178,181].…”

Section: Coiled Coil Domainsmentioning

confidence: 99%

Peptide-based biopolymers in biomedicine and biotechnology

Chow

Nunalee

Lim

et al. 2008

Materials Science and Engineering: R: Reports

280

231

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“…Peptidebased materials have also been shown to form hydrogel networks in response to physical or chemical stimuli. [13][14][15][16][17] These materials utilize the primary modes of self-association in peptides, the hydrophobic aggregation of -strands and coiling of helices, to form long-range networks that lead to hydrogel formation. In this work, we have developed new synthetic block copolymers that respond to both pH and temperature, providing the ability to tune the nanoscale and macroscale structures formed using two independent environmental stimuli, giving rise to unique phase behavior and formation of dehydrated elastic solids by self-assembly that has not been seen in other systems.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Self-Assembly of Multiblock Copolymers in Aqueous Solution

et al. 2006

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A unique pH-dependent phase behavior from a copolytner micellar solution to a collapsed hydrogel with micelles ordered in a hexagonal phase was observed. Small-angle neutron scattering (SANS) was used to follow the pH-dependent structural evolution of micelles formed in a solution of a pentablock copolymer consisting of poly((diethylaminoethyl methacrylate)-b-(ethylene oxide)-b-(propylene oxide)-b-(ethylene oxide)-b-(diethylaminoethyl methacrylate)) (PDEAEM 25-b-PEO 100-b-PPO 65-b-PEO 100-b-PDEAEM 25). Between pH 3.0 and pH 7.4, we observed the presence of charged spherical micelles. Increasing the pH of the micelle solution above pH 7.4 resulted in increasing the size of the micelles due to the increasing hydrophobicity of the PDEAEM blocks above their pK a of 7.6. The increase in size of the spherical micelles resulted in a transition to a cylindrical micelle morphology in the pH range 8.1-10.5, and at pH > 11, the copolymer solution undergoes macroscopic phase separation. Indeed, the phase separated copolymer sediments and coalesces into a hydrogel structure that consists of 25-35 wt% water. Small-angle X-ray scattering (SAXS) clearly indicated that the hydrogel has a hexagonal ordered phase. Interestingly, the process is reversible, as lowering of the pH below 7.0 leads to rapid dissolution of the solid into homogeneous solution. We believe that the hexagonal structure in the hydrogel is a result of the organization of the cylindrical micelles due to the increased hydrophobic interactions between the micelles at 70Â°C and pH 11. Thus we have developed a pH-/ temperature-dependent, reversible hierarchically self-assembling block copolymer system with structures spanning nano-to microscale dimensions. KeywordsAmes Laboratory, copolymer sediments, small-angle neutron scattering, micelles, pH effects, phase separation, self assembly, supramolecular chemistry, block copolymers Disciplines Biochemical and Biomolecular Engineering | Chemical Engineering | Polymer Science CommentsReprinted (adapted) with permission from Langmuir, 22 (4) A unique pH-dependent phase behavior from a copolymer micellar solution to a collapsed hydrogel with micelles ordered in a hexagonal phase was observed. Small-angle neutron scattering (SANS) was used to follow the pHdependent structural evolution of micelles formed in a solution of a pentablock copolymer consisting of poly((diethylaminoethyl methacrylate)-b-(ethylene oxide)-b-(propylene oxide)-b-(ethylene oxide)-b-(diethylaminoethyl methacrylate)) (PDEAEM 25 -b-PEO 100 -b-PPO 65 -b-PEO 100 -b-PDEAEM 25 ). Between pH 3.0 and pH 7.4, we observed the presence of charged spherical micelles. Increasing the pH of the micelle solution above pH 7.4 resulted in increasing the size of the micelles due to the increasing hydrophobicity of the PDEAEM blocks above their pK a of 7.6. The increase in size of the spherical micelles resulted in a transition to a cylindrical micelle morphology in the pH range 8.1-10.5, and at pH >11, the copolymer solution undergoes macroscopic phase separation...

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“…The architecture of such proteins emulates those of naturally occurring protein multimeric structures, mostly based on a class of protein motifs called coiled-coil motifs. Kopecek et al have synthesized hybrid hydrogel systems using coiled-coil domains to drive the self-assembly of polymer chains [81][82][83]. Coiled-coil induced self-assembly has been shown to be strongly dependent on environmental stimuli such as concentration, temperature, pH, and properties of the solvent.…”

Section: Artificial Protein Hydrogelsmentioning

confidence: 99%

“…Specifically, the designed epitope-display model system consisted of two main components: epitope-containing histidine-tagged peptide, and a synthetic polymer with two types of functionalities, one for covalent attachment to the substrate and the other for peptide immobilization [82].…”

Section: Artificial Protein Hydrogelsmentioning

confidence: 99%

Smart polymeric gels: Redefining the limits of biomedical devices

Chaterji

Kwon

Park

2007

Progress in Polymer Science

550

364

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This review describes recent progresses in the development and applications of smart polymeric gels, especially in the context of biomedical devices. The review has been organized into three separate sections: defining the basis of smart properties in polymeric gels; describing representative stimuli to which these gels respond; and illustrating a sample application area, namely, microfluidics. One of the major limitations in the use of hydrogels in stimuli-responsive applications is the diffusion rate limited transduction of signals. This can be obviated by engineering interconnected pores in the polymer structure to form capillary networks in the matrix and by downscaling the size of hydrogels to significantly decrease diffusion paths. Reducing the lag time in the induction of smart responses can be highly useful in biomedical devices, such as sensors and actuators. This review also describes molecular imprinting techniques to fabricate hydrogels for specific molecular recognition of target analytes. Additionally, it describes the significant advances in bottom-up nanofabrication strategies, involving supramolecular chemistry. Learning to assemble supramolecular structures from nature has led to the rapid prototyping of functional supramolecular devices. In essence, the barriers in the current performance potential of biomedical devices can be lowered or removed by the rapid convergence of interdisciplinary technologies.

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The coiled coils in the design of protein-based constructs: hybrid hydrogels and epitope displays

Cited by 53 publications

References 39 publications

Peptide-based biopolymers in biomedicine and biotechnology

Peptide-based biopolymers in biomedicine and biotechnology

Supramolecular Self-Assembly of Multiblock Copolymers in Aqueous Solution

Smart polymeric gels: Redefining the limits of biomedical devices

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