The Cohesion Protein MEI-S332 Localizes to Condensed Meiotic and Mitotic Centromeres until Sister Chromatids Separate

Moore, Daniel P.; Page, Andrea W.; Tang, Tracy Tzu-Ling; Kerrebrock, Anne W.; Orr‐Weaver, Terry L.

doi:10.1083/jcb.140.5.1003

Cited by 121 publications

(105 citation statements)

References 30 publications

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“…Oocytes were stained for DNA with Hoechst and for spindles with anti-a-tubulin conjugated to FITC (Sigma monoclonal antibody DM1A). Additional primary antibodies were rat-anti HA ''high affinity'' (Roche, clone 3F10), rat anti-SUB at 1:75 ( Jang et al 2005), INCENP (1:400) (C. Wu, unpublished data), MEI-S332 (1:1000) (Moore et al 1998) with Cy3 or Cy5 conjugated secondary antibodies preadsorbed against a range of mammalian serum proteins including mouse and rabbit ( Jackson Labs). Images were collected on a Leica TCS SP confocal microscope with a 63X, N.A.…”

Section: Genetic Methodsmentioning

confidence: 99%

mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females

Singaram

McKim

2008

Genetics

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Meiotic chromosome segregation occurs in Drosophila oocytes on an acentrosomal spindle, which raises interesting questions regarding spindle assembly and function. One is how to organize a bipolar spindle without microtubule organizing centers at the poles. Another question is how to orient the chromosomes without kinetochore capture of microtubules that grow from the poles. We have characterized the mei-38 gene in Drosophila and found it may be required for chromosome organization within the karyosome. Nondisjunction of homologous chromosomes occurs in mei-38 mutants primarily at the first meiotic division in females but not in males where centrosomes are present. Most meiotic spindles in mei-38 oocytes are bipolar but poorly organized, and the chromosomes appear disorganized at metaphase. mei-38 encodes a novel protein that is conserved in the Diptera and may be a member of a multigene family. Mei-38 was previously identified (as ssp1) due to a role in mitotic spindle assembly in a Drosophila cell line. MEI-38 protein localizes to a specific population of spindle microtubules, appearing to be excluded from the overlap of interpolar microtubules in the central spindle. We suggest MEI-38 is required for the stability of parallel microtubules, including the kinetochore microtubules.

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Section: Genetic Methodsmentioning

confidence: 99%

mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females

Singaram

McKim

2008

Genetics

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“…gate, while the sister-chromaid arms show bridging at anaphase (Coelho et al 2003 In addition to abnormal centromere separation, the at prometaphase, but concomitant with the separation of sister chromatids, delocalizes from the centromeres at metaphase figures contained sister-chromatid arms that failed to resolve and therefore the two sister-chromatid anaphase (Moore et al 1998). In dcap-g K1 mutant embryos, MEI-S332 localized normally onto prometaphase arms could not be distinguished (Figure 3, G-I).…”

Section: Centromeric Proteins Localize Appropriately Duringmentioning

confidence: 99%

Mutations in the Drosophila Condensin Subunit dCAP-G

2004

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Chromosomes are dynamic structures that are reorganized during the cell cycle to optimize them for distinct functions. SMC and non-SMC condensin proteins associate into complexes that have been implicated in the process of chromosome condensation. The roles of the individual non-SMC subunits of the complex are poorly understood, and mutations in the CAP-G subunit have not been described in metazoans. Here we elucidate a role for dCAP-G in chromosome condensation and cohesion in Drosophila. We illustrate the requirement of dCAP-G for condensation during prophase and prometaphase; however, we find that alternate mechanisms ensure that replicated chromosomes are condensed prior to metaphase. In contrast, dCAP-G is essential for chromosome condensation in metaphase of single, unreplicated sister chromatids, suggesting that there is an interplay between replicated chromatids and the condensin complex. In the dcap-g mutants, defects in sister-chromatid separation are also observed. Chromatid arms fail to resolve in prophase and are unable to separate at anaphase, whereas sister centromeres show aberrant separation in metaphase and successfully move to spindle poles at anaphase. We also identified a role for dCAP-G during interphase in regulating heterochromatic gene expression.

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“…The Drosophila MEI-S332 protein localizes to centromeres in mitosis and meiosis, and is required for the maintenance of centromere cohesion in meiosis II (Kerrebrock et al, 1995;Moore et al;. Since in mitotic chromosomes this protein appears at the centromere pairing domain (Lopez et al, 2000), as does DSA1, we double immunolabeled the two proteins to test for colocalization (Fig.…”

Section: Dsa1 and Mei-s332 Are Found In Distinct Centromere Domainsmentioning

confidence: 99%

Drosophila cohesins DSA1 and Drad21 persist and colocalize along the centromeric heterochromatin during mitosis

Valdeolmillos

Rufas

Suja

et al. 2004

Biology of the Cell

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Sister chromatid cohesion in eukaryotes is maintained mainly by a conserved multiprotein complex termed cohesin. Drad21 and DSA1 are the Drosophila homologues of the yeast Scc1 and Scc3 cohesin subunits, respectively. We recently identified a Drosophila mitotic cohesin complex composed of Drad21/DSA1/DSMC1/DSMC3. Here we study the contribution of this complex to sister chromatid cohesion using immunofluorescence microscopy to analyze cell cycle chromosomal localization of DSA1 and Drad21 in S2 cells. We observed that DSA1 and Drad21 colocalize during all cell cycle stages in cultured cells. Both proteins remain in the centromere until metaphase, colocalizing at the centromere pairing domain that extends along the entire heterochromatin; the centromeric cohesion protein MEI-S332 is nonetheless reported in a distinct centromere domain. These results provide strong evidence that DSA1 and Drad21 are partners in a cohesin complex involved in the maintenance of sister chromatid arm and centromeric cohesion during mitosis in Drosophila.

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The Cohesion Protein MEI-S332 Localizes to Condensed Meiotic and Mitotic Centromeres until Sister Chromatids Separate

Cited by 121 publications

References 30 publications

mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females

mei-38 Is Required for Chromosome Segregation During Meiosis in Drosophila Females

Mutations in the Drosophila Condensin Subunit dCAP-G

Drosophila cohesins DSA1 and Drad21 persist and colocalize along the centromeric heterochromatin during mitosis

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