The Co-4 locus on chromosome Pv08 contains a unique cluster of 18 COK-4 genes and is regulated by immune response in common bean

Oblessuc, Paula Rodrigues; Francisco, Camila; Melotto, Maeli

doi:10.1007/s00122-015-2500-6

Cited by 38 publications

(53 citation statements)

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“…Anthracnose resistance is dominant at all loci except the Co-8 locus, and multiple alleles have been identified at the Co-1 , Co-3 , Co-4 , and Co-5 loci. Fine mapping of some of these resistance genes, Co-x [25]; Co-1 [23]; Co-1 2 [26] and the Co-4 2 [27, 28] has been conducted and candidate genes associated with resistance identified. The Co-x gene was fine mapped to Pv01, adjacent to the Co-1 locus [25] and fine mapping of the Co-4 ( COK-4 ) locus revealed 18 copies of the COK-4 gene in a 325 kb segment of Pv08 [28].…”

Section: Introductionmentioning

confidence: 99%

“…Fine mapping of some of these resistance genes, Co-x [25]; Co-1 [23]; Co-1 2 [26] and the Co-4 2 [27, 28] has been conducted and candidate genes associated with resistance identified. The Co-x gene was fine mapped to Pv01, adjacent to the Co-1 locus [25] and fine mapping of the Co-4 ( COK-4 ) locus revealed 18 copies of the COK-4 gene in a 325 kb segment of Pv08 [28]. Using this information, breeders have been developing anthracnose resistant cultivars for both subsistence and commercial producers [13].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem

2016

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Bean (Phaseolus vulgaris) anthracnose caused by the hemi-biotrophic pathogen Colletotrichum lindemuthianum is a major factor limiting production worldwide. Although sources of resistance have been identified and characterized, the early molecular events in the host-pathogen interface have not been investigated. In the current study, we conducted a comprehensive transcriptome analysis using Illumina sequencing of two near isogenic lines (NILs) differing for the presence of the Co-1 gene on chromosome Pv01 during a time course following infection with race 73 of C. lindemuthianum. From this, we identified 3,250 significantly differentially expressed genes (DEGs) within and between the NILs over the time course of infection. During the biotrophic phase the majority of DEGs were up regulated in the susceptible NIL, whereas more DEGs were up-regulated in the resistant NIL during the necrotrophic phase. Various defense related genes, such as those encoding PR proteins, peroxidases, lipoxygenases were up regulated in the resistant NIL. Conversely, genes encoding sugar transporters were up-regulated in the susceptible NIL during the later stages of infection. Additionally, numerous transcription factors (TFs) and candidate genes within the vicinity of the Co-1 locus were differentially expressed, suggesting a global reprogramming of gene expression in and around the Co-1 locus. Through this analysis, we reduced the previous number of candidate genes reported at the Co-1 locus from eight to three. These results suggest the dynamic nature of P. vulgaris–C. lindemuthianum interaction at the transcriptomic level and reflect the role of both pathogen and effector triggered immunity on changes in plant gene expression.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem

2016

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“…The PhaseolusGenes marker database has become an essential tool for cataloging bean marker diversity so that the breeding community can utilize marker information from multiple sources to facilitate MAS (Gonçalves-Vidigal et al, 2011Reinprecht et al, 2013;Oblessuc et al, 2013Oblessuc et al, , 2015Aranda et al, 2014;Keller et al, 2015;Sousa et al, 2015;Coimbra-Gonçalves et al, 2016;Perseguini et al, 2016). Because genomic resequencing of breeding parents is today a more routine practice with the lowered cost of sequencing, the PhaseolusGenes database should evolve from a marker database into a sequence database, which includes not only the current markers but also the increasingly large number (> 2,000) of genotypes that have been sequenced, including the 16 parental genotypes of the ABC projects (T. Miller and P. Gepts, unpubl.…”

Section: Discussionmentioning

confidence: 99%

“…Quantitative trait locus (QTL) mapping had also been used to identify multiple resistance loci in Andean germplasm. Seven QTLs on five chromosomes were identified in a IAC-UNA × CAL 143 recombinant inbred population and named ALS2.1, ALS3.1, ALS4.1, ALS4.2, ALS5.1, ALS5.2 and ALS10.1 (Oblessuc et al, 2012(Oblessuc et al, , 2013(Oblessuc et al, , 2015. Additionally, three major R genes identified in Andean G5686 (Phg G5686A , Phg G5686B and Phg G5686C ) (Mahuku et al, 2009) were later treated as quantitative loci by Keller et al (2015) and mapped to the same loci as ALS4.1, ALS4.2 and ALS9.1 (Oblessuc et al, 2012;Keller et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

Miller¹,

Gepts²,

Kimno³

et al. 2018

Afr. J. Biotechnol.

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Common bean (Phaseolus vulgaris) is a rich source of protein, vitamins, and micronutrients and is an important crop for food security throughout Latin America, Asia, and Africa. Among tropical and subtropical regions of the world, where the majority of beans are grown, yield losses due to the pathogenic fungus Pseudocercospora griseola, causing angular leaf spot (ALS), can be as high as 80%. The strategic use of marker-assisted selection (MAS) to pyramid multiple resistance genes into a single genetic background with preferred morphological and cooking characteristics is being implemented by six research groups throughout East Africa that make up the African Bean Consortium (ABC). Identifying unique markers that are polymorphic among multiple parents is a major source of marker attrition. In this study, an illustration of how 22 DNA sequences physically linked to the Phg-2 ALS resistance locus were identified using the PhaseolusGenes marker database and checked for amplification and polymorphism among 16 ABC breeding parents are given. Only three polymorphic markers could be identified following this procedure; one (g796), showed a polymorphism present only in the ALS resistance donor, Mexico 54. The PCR protocol developed to identify the g796 polymorphism was validated among five laboratories. Furthermore, co-segregation analysis of the marker and ALS resistance phenotype in a population of 100 F 2 individuals from the cross between French bean (that is, green bean) genotype Amy and ALS resistance donor Mexico 54 showed the marker is genetically linked (3 cM) to the Phg-2 locus, in addition to being physically linked. This study suggests that in the near future, genetic resequencing data of diverse common bean accessions, compiled within an easily accessible database, will facilitate identification of markers for MAS, marker/trait association, and candidate gene identification.

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“…The Co-4 locus contains several paralogs of the COK-4 gene that is predicted to code for a receptor-like kinase (RLK). The predicted COK-4 protein is highly similar to FERONIA (FER), a membrane-localized protein of Arabidopsis, as they cluster in a single clade of the RLK phylogenetic tree (Melotto and Kelly, 2001;Melotto et al, 2004;Oblessuc et al, 2015). Receptor-like kinases (RLKs) are important pattern recognition receptor (PRRs) that play a role in self and non-self-recognition, including the perception of hormones (Shiu and Bleecker, 2001), pathogen associated molecular patterns (PAMPs) and pathogen effectors.…”

Section: Joint Analysis Between Incompatibility Reaction To Races 73 mentioning

confidence: 99%

Common bean germplasm resistant to races 73 and 2047 of Colletotrichum lindemuthianum

Darben¹,

Gonela²,

Elias³

et al. 2017

Afr. J. Biotechnol.

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The Co-4 locus on chromosome Pv08 contains a unique cluster of 18 COK-4 genes and is regulated by immune response in common bean

Cited by 38 publications

References 50 publications

Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem

Transcriptome Profiling of the Phaseolus vulgaris - Colletotrichum lindemuthianum Pathosystem

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

Common bean germplasm resistant to races 73 and 2047 of Colletotrichum lindemuthianum

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