The CmTCP20 gene regulates petal elongation growth in Chrysanthemum morifolium

Wang, Jingjing; Guan, Yunxiao; Ding, Lian; Li, Pirui; Zhao, Wenqian; Jiang, Jiafu; Chen, Sumei; Chen, Fadi

doi:10.1016/j.plantsci.2018.12.008

Cited by 34 publications

(21 citation statements)

References 26 publications

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“…To further examine the function of the polarity gene YAB1 homolog ( CmYAB1 ) in chrysanthemum, CmYAB1 driven by the cauliflower mosaic virus (CaMV) 35S promoter was introduced to ‘Jinba’, a cultivar of chrysanthemum with spoon‐like ray flowers and decorative inflorescence by Agrobacterium‐mediated leaf disc transformation (Wang et al ., ). We obtained thirteen transgenic lines, and three of nine lines with similar phenotype were chosen for further analysis.…”

Section: Resultsmentioning

confidence: 99%

“…The CmYAB1 coding sequence was first cloned into a pENTR 1A vector and then introduced into the pMDC43 vector by LR recombination. The pMDC43-CmYAB1 vector was introduced to the chrysanthemum 'Jinba' by Agrobacterium-mediated genetic transformation as described previously (Wang et al, 2019). After further screening by PCR at the DNA level, the positive transgenic seedlings were grown in a growth chamber for a week under 16 h/8 h and 25°C/18°C day/night and were then transferred to greenhouse under standard management.…”

Section: Cmyab1 Genetic Transformationmentioning

confidence: 99%

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Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

Ding

Zhao

Zhang

et al. 2019

Plant Biotechnology Journal

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Summary The diversity of form of the chrysanthemum flower makes this species an ideal model for studying petal morphogenesis, but as yet, the molecular mechanisms underlying petal shape development remain largely unexplored. Here, a floral mutant, which arose as a bud sport in a plant of the variety ‘Anastasia Dark Green’, and formed straight, rather than hooked petals, was subjected to both comparative morphological analysis and transcriptome profiling. The hooked petals only became discernible during a late stage of flower development. At the late stage of ‘Anastasia Dark Green’, genes related to chloroplast, hormone metabolism, cell wall and microtubules were active, as were cell division‐promoting factors. Auxin concentration was significantly reduced, and a positive regulator of cell expansion was down‐regulated. Two types of critical candidates, boundary genes and adaxial–abaxial regulators, were identified from 7937 differentially expressed genes in pairwise comparisons, which were up‐regulated at the late stage in ‘Anastasia Dark Green’ and another two hooked varieties. Ectopic expression of a candidate abaxial gene, CmYAB1, in chrysanthemum led to changes in petal curvature and inflorescence morphology. Our findings provide new insights into the regulatory networks underlying chrysanthemum petal morphogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Cmyab1 Genetic Transformationmentioning

confidence: 99%

Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

Ding

Zhao

Zhang

et al. 2019

Plant Biotechnology Journal

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“…For example, AtTCP20 is involved in stimulation of cell growth and division [73]. Overexpressing CmTCP20 in Arabidopsis and chrysanthemum plants exhibit larger inflorescences and flower buds and longer petal length [74]. Again, the expression patterns of PaTCP14a/b and PaTCP19b during the petal development support their functions to promote cell proliferation, while higher expression in the petals of small flowers is contrary to the promoting role in petal growth, which needs further study to be understood.…”

Section: Potential Roles Of Patcp Genes During Petal Developmentmentioning

confidence: 99%

Genome-Wide Identification, Characterization and Expression Analysis of TCP Transcription Factors in Petunia

Zhang

Zhou

Chen

et al. 2020

IJMS

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The plant-specific TCP transcription factors are well-characterized in both monocots and dicots, which have been implicated in multiple aspects of plant biological processes such as leaf morphogenesis and senescence, lateral branching, flower development and hormone crosstalk. However, no systematic analysis of the petunia TCP gene family has been described. In this work, a total of 66 petunia TCP genes (32 PaTCP genes in P. axillaris and 34 PiTCP genes in P. inflata) were identified. Subsequently, a systematic analysis of 32 PaTCP genes was performed. The phylogenetic analysis combined with structural analysis clearly distinguished the 32 PaTCP proteins into two classes—class Ι and class Ⅱ. Class Ⅱ was further divided into two subclades, namely, the CIN-TCP subclade and the CYC/TB1 subclade. Plenty of cis-acting elements responsible for plant growth and development, phytohormone and/or stress responses were identified in the promoter of PaTCPs. Distinct spatial expression patterns were determined among PaTCP genes, suggesting that these genes may have diverse regulatory roles in plant growth development. Furthermore, differential temporal expression patterns were observed between the large- and small-flowered petunia lines for most PaTCP genes, suggesting that these genes are likely to be related to petal development and/or petal size in petunia. The spatiotemporal expression profiles and promoter analysis of PaTCPs indicated that these genes play important roles in petunia diverse developmental processes that may work via multiple hormone pathways. Moreover, three PaTCP-YFP fusion proteins were detected in nuclei through subcellular localization analysis. This is the first comprehensive analysis of the petunia TCP gene family on a genome-wide scale, which provides the basis for further functional characterization of this gene family in petunia.

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“…Flower formation involves the development of sepals, petals, stamens, and pistils, regulated by several genes involved in flower organ identity 34 . The overexpression of CmTCP20, a member of teosinte branched1/cycloidea/proliferating cell factors (TCPs) gene family, led to larger flower inflorescences and longer petals in chrysanthemum 46 . Overexpression of CmCYC2c in Chrysanthemum lavandulifolium led to significant increase in flower numbers and petal ligule length of ray florets 47 .…”

Section: Flower Anatomy Modificationmentioning

confidence: 99%

Overview and detectability of the genetic modifications in ornamental plants

Boutigny¹,

Dohin²,

Pornin³

et al. 2020

Hortic Res

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The market of ornamental plants is extremely competitive, and for many species genetic engineering can be used to introduce original traits of high commercial interest. However, very few genetically modified (GM) ornamental varieties have reached the market so far. Indeed, the authorization process required for such plants has a strong impact on the profitability of the development of such products. Considering the numerous scientific studies using genetic modification on ornamental species of interest, a lot of transformed material has been produced, could be of commercial interest and could therefore be unintentionally released on the market. The unintentional use of GM petunia in breeding programs has indeed recently been observed. This review lists scientific publications using GM ornamental plants and tries to identify whether these plants could be detected by molecular biology tools commonly used by control laboratories.

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The CmTCP20 gene regulates petal elongation growth in Chrysanthemum morifolium

Cited by 34 publications

References 26 publications

Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

Comprehensive characterization of a floral mutant reveals the mechanism of hooked petal morphogenesis in Chrysanthemum morifolium

Genome-Wide Identification, Characterization and Expression Analysis of TCP Transcription Factors in Petunia

Overview and detectability of the genetic modifications in ornamental plants

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